TALENs: A widely applicable technology for targeted genome editing
Open accessAbstract
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The discovery that TALEs use a simple modular code for DNA recognition has provided an alternative platform for genome editing and gene disruption.
“The discovery that TALEs use a simple modular code for DNA recognition has provided an alternative platform for genome editing and gene disruption.”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [66]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., & Bonas, U. (2009) · Science · doi:10.1126/science.1178811 · Cited by 2,707 · see citing works
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285 · see citing works
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.
Kim, Y. G., Cha, J., & Chandrasegaran, S. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1156 · Cited by 2,099 · see citing works
A TALE nuclease architecture for efficient genome editing
Miller, J. C., Tan, S., Qiao, G., Barlow, K. A., Wang, J., Xia, D. F., Meng, X., Paschon, D. E., Leung, E., Hinkley, S. J., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1755 · Cited by 2,098 · see citing works
Čermák, T., Doyle, E., Christian, M. L., Wang, L., Zhang, Y., Schmidt, C. L., Baller, J. A., Somia, N. V., Bogdanove, A. J., & Voytas, D. F. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr218 · Cited by 2,034 · see citing works
Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Christian, M. L., Čermák, T., Doyle, E., Schmidt, C. L., Zhang, F., Hummel, A. W., Bogdanove, A. J., & Voytas, D. F. (2010) · Genetics · doi:10.1534/genetics.110.120717 · Cited by 2,033 · see citing works
A Simple Cipher Governs DNA Recognition by TAL Effectors
Moscou, M., & Bogdanove, A. J. (2009) · Science · doi:10.1126/science.1178817 · Cited by 1,992 · see citing works
Highly efficient endogenous human gene correction using designed zinc-finger nucleases
Urnov, F. D., Miller, J. C., Lee, Y.-L., Beausejour, C. M., Rock, J. M., Augustus, S., Jamieson, A. C., Porteus, M. H., Gregory, P. D., & Holmes, M. C. (2005) · Nature · doi:10.1038/nature03556 · Cited by 1,642 · see citing works
Vascular Endothelial Growth Factor
Ferrara, N. (2009) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.108.179663 · Cited by 1,283 · see citing works
FLASH assembly of TALENs for high-throughput genome editing
Reyon, D., Tsai, S. Q., Khayter, C., Foden, J. A., Sander, J. D., & Joung, J. K. (2012) · Nature Biotechnology · doi:10.1038/nbt.2170 · Cited by 1,171 · see citing works
High-efficiency TALEN-based gene editing produces disease-resistant rice
Li, T., Liu, B., Spalding, M. H., Weeks, D. P., & Yang, B. (2012) · Nature Biotechnology · doi:10.1038/nbt.2199 · Cited by 1,149 · see citing works
Genetic engineering of human pluripotent cells using TALE nucleases
Hockemeyer, D. F., Wang, H., Kiani, S., Lai, C. S., Gao, Q., Cassady, J. P., Cost, G. J., Zhang, L., Santiago, Y., Miller, J. C., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1927 · Cited by 1,129 · see citing works
DNA Recognition by Cys<sub>2</sub>His<sub>2</sub> Zinc Finger Proteins
Wolfe, S. A., Nekludova, L., & Pabo, C. O. (2000) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.29.1.183 · Cited by 1,053 · see citing works
Hockemeyer, D. F., Soldner, F., Beard, C., Gao, Q., Mitalipova, M. M., DeKelver, R. C., Katibah, G. E., Amora, R., Boydston, E. A., Zeitler, B., et al. (2009) · Nature Biotechnology · doi:10.1038/nbt.1562 · Cited by 1,035 · see citing works
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
Perez, E. E., Wang, J., Miller, J. C., Jouvenot, Y., Kim, K. A., Liu, O., Wang, N., Lee, G., Bartsevich, V. V., Lee, Y.-L., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1410 · Cited by 999 · see citing works
<i>Xanthomonas</i> AvrBs3 Family-Type III Effectors: Discovery and Function
Boch, J., & Bonas, U. (2010) · Annual Review of Phytopathology · doi:10.1146/annurev-phyto-080508-081936 · Cited by 978 · see citing works
Precise genome modification in the crop species Zea mays using zinc-finger nucleases
Shukla, V. K., Doyon, Y., Miller, J. C., DeKelver, R. C., Moehle, E. A., Worden, S. E., Mitchell, J. C., Arnold, N. L., Gopalan, S. M., Meng, X., et al. (2009) · Nature · doi:10.1038/nature07992 · Cited by 963 · see citing works
Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases
Geurts, A. M., Cost, G. J., Freyvert, Y., Zeitler, B., Miller, J. C., Choi, V. M., Jenkins, S. S., Wood, A. J., Cui, X., Meng, X., et al. (2009) · Science · doi:10.1126/science.1172447 · Cited by 924 · see citing works
In vivo genome editing using a high-efficiency TALEN system
Bedell, V. M., Wang, Y., Campbell, J. M., Poshusta, T. L., Starker, C. G., Krug, R. G., Tan, W. S., Penheiter, S. G., Ma, A. C. H., Leung, A. Y. H., et al. (2012) · Nature · doi:10.1038/nature11537 · Cited by 914 · see citing works
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
Doyon, Y., McCammon, J. M., Miller, J. C., Faraji, F., Ngo, C., Katibah, G. E., Amora, R., Hocking, T. D., Zhang, L., Rebar, E. J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1409 · Cited by 896 · see citing works
Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
Bibikova, M., Golic, M., Golic, K. G., & Carroll, D. (2002) · Genetics · doi:10.1093/genetics/161.3.1169 · Cited by 860 · see citing works
Lombardo, A., Genovese, P., Beausejour, C. M., Colleoni, S., Lee, Y.-L., Kim, K. A., Ando, D., Urnov, F. D., Galli, C., Gregory, P. D., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1353 · Cited by 845 · see citing works
Enhancing Gene Targeting with Designed Zinc Finger Nucleases
Bibikova, M. V., Beumer, K. J., Trautman, J. K., & Carroll, D. (2003) · Science · doi:10.1126/science.1079512 · Cited by 824 · see citing works
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription
Zhang, F., Cong, L., Lodato, S., Kosuri, S., Church, G. M., & Arlotta, P. (2011) · Nature Biotechnology · doi:10.1038/nbt.1775 · Cited by 771 · see citing works
Chimeric nucleases stimulate gene targeting in human cells
Porteus, M. H., & Baltimore, D. (2003) · Science · doi:10.1126/science.1078395 · Cited by 741 · see citing works
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
Meng, X., Noyes, M. B., Zhu, L. J., Lawson, N. D., & Wolfe, S. A. (2008) · Nature Biotechnology · doi:10.1038/nbt1398 · Cited by 741 · see citing works
High-frequency modification of plant genes using engineered zinc-finger nucleases
Townsend, J. A., Wright, D. A., Winfrey, R. J., Fu, F., Maeder, M. L., Joung, J. K., & Voytas, D. F. (2009) · Nature · doi:10.1038/nature07845 · Cited by 737 · see citing works
Soldner, F., Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan, R., Khurana, V., Golbe, L. I., Myers, R. H., Lindquist, S., et al. (2011) · Cell · doi:10.1016/j.cell.2011.06.019 · Cited by 728 · see citing works
Design and Selection of Novel Cys2His2 Zinc Finger Proteins
Pabo, C. O., Peisach, E., & Grant, R. A. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.313 · Cited by 715 · see citing works
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
Mussolino, C., Morbitzer, R., Lütge, F., Dannemann, N., Lahaye, T., & Cathomen, T. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr597 · Cited by 714 · see citing works
Maeder, M. L., Thibodeau-Beganny, S., Osiak, A., Wright, D. A., Anthony, R. M., Eichtinger, M., Jiang, T., Foley, J. E., Winfrey, R. J., Townsend, J. A., et al. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.06.016 · Cited by 708 · see citing works
Targeted Genome Editing Across Species Using ZFNs and TALENs
Wood, A. J., Lo, T., Zeitler, B., Pickle, C. S., Ralston, E. J., Lee, A. H., Amora, R., Miller, J. C., Leung, E., Meng, X., et al. (2011) · Science · doi:10.1126/science.1207773 · Cited by 664 · see citing works
Heritable gene targeting in zebrafish using customized TALENs
Huang, P., Xiao, A., Zhou, M., Zhu, Z., Lin, S., & Zhang, B. (2011) · Nature Biotechnology · doi:10.1038/nbt.1939 · Cited by 658 · see citing works
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
Yusa, K., Rashid, S. T., Strick‐Marchand, H., Varela, I., Liu, P.-Q., Paschon, D. E., Miranda, E., Ordóñez, A., Hannan, N. R. F., Rouhani, F. J., et al. (2011) · Nature · doi:10.1038/nature10424 · Cited by 632 · see citing works
A transcription activator-like effector toolbox for genome engineering
Sanjana, N. E., Cong, L., Zhou, Y., Cunniff, M. M., Feng, G., & Zhang, F. (2012) · Nature Protocols · doi:10.1038/nprot.2011.431 · Cited by 628 · see citing works
Targeted gene disruption in somatic zebrafish cells using engineered TALENs
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., & Yeh, J.-R. J. (2011) · Nature Biotechnology · doi:10.1038/nbt.1934 · Cited by 622 · see citing works
Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors
Deng, D., Yan, C., Pan, X., Mahfouz, M. M., Wang, J., Zhu, J., Shi, Y., & Yan, N. (2012) · Science · doi:10.1126/science.1215670 · Cited by 601 · see citing works
TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction
Doyle, E., Booher, N. J., Standage, D., Voytas, D. F., Brendel, V., VanDyk, J. K., & Bogdanove, A. J. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks608 · Cited by 587 · see citing works
Knockout rats generated by embryo microinjection of TALENs
Tesson, L., Usal, C., Ménoret, S., Leung, E., Niles, B. J., Remy, S., Santiago, Y., Vincent, A. I., Meng, X., Zhang, L., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1940 · Cited by 585 · see citing works
Efficient TALEN-mediated gene knockout in livestock
Carlson, D. F., Tan, W. S., Lillico, S. G., Štveráková, D., Proudfoot, C., Christian, M. L., Voytas, D. F., Long, C. R., Whitelaw, B., & Fahrenkrug, S. C. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1211446109 · Cited by 580 · see citing works
The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target
Mak, A. N.-S., Bradley, P. H., Cernadas, R. A., Bogdanove, A. J., & Stoddard, B. L. (2012) · Science · doi:10.1126/science.1216211 · Cited by 569 · see citing works
Yano, A., Guyomard, R. R., Nicol, B., Jouanno, E., Quillet, E., Klopp, C., Cabau, C., Bouchez, O., Fostier, A., & Guiguen, Y. (2012) · Current Biology · doi:10.1016/j.cub.2012.05.045 · Cited by 553 · see citing works
TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain
Li, T., Huang, S., Jiang, W. Z., Wright, D. A., Spalding, M. H., Weeks, D. P., & Yang, B. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq704 · Cited by 547 · see citing works
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)
Sander, J. D., Dahlborg, E. J., Goodwin, M. J., Cade, L., Zhang, F., Cifuentes, D., Curtin, S. J., Blackburn, J. S., Thibodeau-Beganny, S., Qi, Y., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1542 · Cited by 518 · see citing works
Gene Targeting of a Disease-Related Gene in Human Induced Pluripotent Stem and Embryonic Stem Cells
Zou, J., Maeder, M. L., Mali, P., Pruett‐Miller, S. M., Thibodeau-Beganny, S., Chou, B.-K., Chen, G., Ye, Z., Park, I., Daley, G. Q., et al. (2009) · Cell stem cell · doi:10.1016/j.stem.2009.05.023 · Cited by 509 · see citing works
Engineering polydactyl zinc-finger transcription factors
Beerli, R. R., & Barbas, C. F. (2002) · Nature Biotechnology · doi:10.1038/nbt0202-135 · Cited by 495 · see citing works
High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases
Chen, F., Pruett‐Miller, S. M., Huang, Y., Gjoka, M., Duda, K., Taunton, J., Collingwood, T. N., Frödin, M., & Davis, G. D. (2011) · Nature Methods · doi:10.1038/nmeth.1653 · Cited by 475 · see citing works
Unexpected failure rates for modular assembly of engineered zinc fingers
Ramirez, C. L., Foley, J. E., Wright, D. A., Müller-Lerch, F., Rahman, S. H., Cornu, T. I., Winfrey, R. J., Sander, J. D., Fu, F., Townsend, J. A., et al. (2008) · Nature Methods · doi:10.1038/nmeth0508-374 · Cited by 426 · see citing works
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
Lloyd, A., Plaisier, C. L., Carroll, D., & Drews, G. N. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409339102 · Cited by 418 · see citing works
Li, T., Huang, S., Zhao, X., Wright, D. A., Carpenter, S. L., Spalding, M. H., Weeks, D. P., & Yang, B. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr188 · Cited by 414 · see citing works
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures
Doyon, Y., Vo, T. D., Mendel, M. C., Greenberg, S. G., Wang, J., Xia, D. F., Miller, J. C., Urnov, F. D., Gregory, P. D., & Holmes, M. C. (2011) · Nature Methods · doi:10.1038/nmeth.1539 · Cited by 402 · see citing works
High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases
Zhang, F., Maeder, M. L., Unger‐Wallace, E., Hoshaw, J. P., Reyon, D., Christian, M. L., Li, X., Pierick, C. J., Dobbs, D. L., Peterson, T., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914991107 · Cited by 379 · see citing works
Moehle, E. A., Rock, J. M., Lee, Y.-L., Jouvenot, Y., DeKelver, R. C., Gregory, P. D., Urnov, F. D., & Holmes, M. C. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0611478104 · Cited by 371 · see citing works
Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases
Hauschild‐Quintern, J., Petersen, B., Santiago, Y., Queisser, A. L., Carnwath, J. W., Lucas‐Hahn, A., Zhang, L., Meng, X., Gregory, P. D., Schwinzer, R., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1106422108 · Cited by 366 · see citing works
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases
Santiago, Y., Chan, E. M., Liu, P., Orlando, S., Zhang, L., Urnov, F. D., Holmes, M. C., Guschin, D. Y., Waite, A. J., Miller, J. C., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0800940105 · Cited by 358 · see citing works
Cong, L., Zhou, R., Kuo, Y.-C., Cunniff, M. M., & Zhang, F. (2012) · Nature Communications · doi:10.1038/ncomms1962 · Cited by 338 · see citing works
Targeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases
Curtin, S. J., Zhang, F., Sander, J. D., Haun, W. J., Starker, C. G., Baltes, N. J., Reyon, D., Dahlborg, E. J., Goodwin, M. J., Coffman, A. P., et al. (2011) · PLANT PHYSIOLOGY · doi:10.1104/pp.111.172981 · Cited by 333 · see citing works
A General Strategy for Selecting High-Affinity Zinc Finger Proteins for Diverse DNA Target Sites
Greisman, H. A., & Pabo, C. O. (1997) · Science · doi:10.1126/science.275.5300.657 · Cited by 331 · see citing works
Simsek, D., Brunet, E., Wong, S., Katyal, S., Gao, Y., McKinnon, P. J., Lou, J. F., Zhang, L., Li, J., Rebar, E. J., et al. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1002080 · Cited by 327 · see citing works
Targeted integration in rat and mouse embryos with zinc-finger nucleases
Cui, X., Ji, D., Fisher, D. A. C., Wu, Y., Briner, D. M., & Weinstein, E. J. (2010) · Nature Biotechnology · doi:10.1038/nbt.1731 · Cited by 312 · see citing works
Sebastiano, V., Maeder, M. L., Angstman, J. F., Haddad, B., Khayter, C., Yeo, D. T., Goodwin, M. J., Hawkins, J. S., Ramirez, C. L., Batista, L. F. Z., et al. (2011) · Stem Cells · doi:10.1002/stem.718 · Cited by 307 · see citing works
Targeted gene knockout by direct delivery of zinc-finger nuclease proteins
Gaj, T., Guo, J., Kato, Y., Sirk, S. J., & Barbas, C. F. (2012) · Nature Methods · doi:10.1038/nmeth.2030 · Cited by 306 · see citing works
TAL effector RVD specificities and efficiencies
Streubel, J., Blücher, C., Landgraf, A., & Boch, J. (2012) · Nature Biotechnology · doi:10.1038/nbt.2304 · Cited by 294 · see citing works
Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
Meyer, M., de Angelis, M. H., Wurst, W., & Kühn, R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1009424107 · Cited by 286 · see citing works
Morbitzer, R., Römer, P., Boch, J., & Lahaye, T. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1013133107 · Cited by 283 · see citing works
Targeted Genome Modification in Mice Using Zinc-Finger Nucleases
Carbery, I. D., Ji, D., Harrington, A. N., Brown, V. E., Weinstein, E. J., Liaw, L., & Cui, X. (2010) · Genetics · doi:10.1534/genetics.110.117002 · Cited by 274 · see citing works
Targeted chromosomal deletions in human cells using zinc finger nucleasesPreprint
Lee, H. J., Kim, E., & Kim, J. (2009) · Genome Research · doi:10.1101/gr.099747.109 · Cited by 250 · see citing works
Cade, L., Reyon, D., Hwang, W. Y., Tsai, S. Q., Patel, S., Khayter, C., Joung, J. K., Sander, J. D., Peterson, R. T., & Yeh, J.-R. J. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks518 · Cited by 243 · see citing works
Lei, Y., Guo, X., Liu, Y., Cao, Y., Deng, Y., Chen, X., Cheng, C. H. K., Dawid, I. B., Chen, Y., & Zhao, H. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215421109 · Cited by 242 · see citing works
Liu, P., Rebar, E. J., Zhang, L., Liu, Q., Jamieson, A. C., Liang, Y., Qi, H., Li, P.-X., Chen, B., Mendel, M. C., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011172200 · Cited by 239 · see citing works
Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy
Liu, J., Li, C., Yu, Z., Huang, P., Wu, H., Wei, C., Zhu, N., Shen, Y., Chen, Y., Zhang, B., et al. (2012) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2012.04.003 · Cited by 209 · see citing works
Surrogate reporters for enrichment of cells with nuclease-induced mutations
Kim, H., Um, E., Cho, S., Jung, C., Kim, J., & Kim, J. (2011) · Nature Methods · doi:10.1038/nmeth.1733 · Cited by 208 · see citing works
Chromosomal translocations induced at specified loci in human stem cells
Brunet, E., Simsek, D., Tomishima, M. J., DeKelver, R. C., Choi, V. M., Gregory, P. D., Urnov, F. D., Weinstock, D. M., & Jasin, M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0902076106 · Cited by 206 · see citing works
Nontransgenic Genome Modification in Plant Cells
Marton, I., Zuker, A., Shklarman, E., Zeevi, V., Tovkach, A., Roffe, S., Ovadis, M. I., Tzfira, T., & Vainstein, A. (2010) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.164806 · Cited by 188 · see citing works
Bultmann, S., Morbitzer, R., Schmidt, C. S., Thanisch, K., Spada, F., Elsäesser, J., Lahaye, T., & Leonhardt, H. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks199 · Cited by 184 · see citing works
Morton, J. J., Davis, M. W., Jørgensen, E. M., & Carroll, D. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605633103 · Cited by 181 · see citing works
Wolfe, S. A., Greisman, H. A., Ramm, E. I., & Pabo, C. O. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.2421 · Cited by 177 · see citing works
Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects
Ramirez, C. L., Certo, M. T., Mussolino, C., Goodwin, M. J., Cradick, T. J., McCaffrey, A. P., Cathomen, T., Scharenberg, A. M., & Joung, J. K. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks179 · Cited by 174 · see citing works
Flisikowska, T., Thorey, I. S., Offner, S., Ros, F., Lifke, V., Zeitler, B., Rottmann, O., Vincent, A. I., Zhang, L., Jenkins, S. S., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0021045 · Cited by 171 · see citing works
Designing Transcription Factor Architectures for Drug Discovery
Blancafort, P., Segal, D. J., & Barbas, C. F. (2004) · Molecular Pharmacology · doi:10.1124/mol.104.002758 · Cited by 171 · see citing works
Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle
Yu, S., Luo, J., Song, Z., Ding, F., Dai, Y., & Li, N. (2011) · Cell Research · doi:10.1038/cr.2011.153 · Cited by 169 · see citing works
Highly Efficient and Specific Genome Editing in Silkworm Using Custom TALENs
Ma, S., Zhang, S., Wang, F., Liu, Y., Liu, Y., Xu, H., Liu, C., Lin, Y., Zhao, P., & Xia, Q. (2012) · PLoS ONE · doi:10.1371/journal.pone.0045035 · Cited by 168 · see citing works
Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleasesPreprint
Lee, H. J., Kweon, J., Kim, E., Kim, S., & Kim, J. (2011) · Genome Research · doi:10.1101/gr.129635.111 · Cited by 165 · see citing works
Transcriptional Activators of Human Genes with Programmable DNA-Specificity
Geiβler, R., Scholze, H., Hahn, S., Streubel, J., Bonas, U., Behrens, S., & Boch, J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019509 · Cited by 164 · see citing works
Moore, F. E., Reyon, D., Sander, J. D., Martinez, S. A., Blackburn, J. S., Khayter, C., Ramirez, C. L., Joung, J. K., & Langenau, D. M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037877 · Cited by 163 · see citing works
Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection
Takasu, Y., Kobayashi, I., Beumer, K. J., Uchino, K., Sezutsu, H., Sajwan, S., Carroll, D., Tamura, T., & Žurovec, M. (2010) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2010.07.012 · Cited by 161 · see citing works
Engineering synthetic TAL effectors with orthogonal target sites
Garg, A., Lohmueller, J. J., Silver, P. A., & Armel, T. Z. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks404 · Cited by 155 · see citing works
Synergy between adjacent zinc fingers in sequence-specific DNA recognition
Isalan, M., Choo, Y., & Klug, A. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.11.5617 · Cited by 149 · see citing works
Chimeric TALE recombinases with programmable DNA sequence specificity
Mercer, A. C., Gaj, T., Fuller, R. P., & Barbas, C. F. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks875 · Cited by 142 · see citing works
Watanabe, T., Ochiai, H., Sakuma, T., Horch, H. W., Hamaguchi, N., Nakamura, T., Bando, T., Ohuchi, H., Yamamoto, T., Noji, S., et al. (2012) · Nature Communications · doi:10.1038/ncomms2020 · Cited by 139 · see citing works
Precision genome engineering with programmable DNA-nicking enzymesPreprint
Kim, E., Kim, S., Kim, D. H., Choi, B., Choi, I., & Kim, J. (2012) · Genome Research · doi:10.1101/gr.138792.112 · Cited by 137 · see citing works
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzymePreprint
Wang, J., Friedman, G., Doyon, Y., Wang, N. S., Li, C. J., Miller, J. C., Hua, K. L., Yan, J. J., Babiarz, J., Gregory, P. D., et al. (2012) · Genome Research · doi:10.1101/gr.122879.111 · Cited by 135 · see citing works
Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease
Sun, N., Liang, J., Abil, Z., & Zhao, H. (2012) · Molecular BioSystems · doi:10.1039/c2mb05461b · Cited by 134 · see citing works
Young, J. J., Cherone, J. M., Doyon, Y., Ankoudinova, I., Faraji, F., Lee, A. H., Ngo, C., Guschin, D. Y., Paschon, D. E., Miller, J. C., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1102030108 · Cited by 131 · see citing works
Baker, M. (2011) · Nature Methods · doi:10.1038/nmeth.1807 · Cited by 110 · see citing works
Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleasesPreprint
Merlin, C., Beaver, L. E., Taylor, O. R., Wolfe, S. A., & Reppert, S. M. (2012) · Genome Research · doi:10.1101/gr.145599.112 · Cited by 107 · see citing works
Human Umbilical Cord Blood Cells Restore Brain Damage Induced Changes in Rat Somatosensory Cortex
Geißler, M., Dinse, H. R., Neuhoff, S., Kreikemeier, K., & Meier, C. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020194 · Cited by 103 · see citing works
Zinc-Finger Nuclease Based Genome Surgery: Its All About Specificity
Händel, E.-M., & Cathomen, T. (2011) · Current Gene Therapy · doi:10.2174/156652311794520120 · Cited by 101 · see citing works
Dong, Z., Ge, J., Li, K., Xu, Z., Liang, D., Li, J., Li, J., Jia, W., Li, Y., Dong, X., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0028897 · Cited by 85 · see citing works
Targeted mutagenesis in the sea urchin embryo using zinc‐finger nucleases
Ochiai, H., Fujita, K., Suzuki, K., Nishikawa, M., Shibata, T., Sakamoto, N., & Yamamoto, T. (2010) · Genes to Cells · doi:10.1111/j.1365-2443.2010.01425.x · Cited by 84 · see citing works
An Integrated Chip for the High‐Throughput Synthesis of Transcription Activator‐like Effectors
Wang, Z., Li, J., Huang, H., Wang, G., Jiang, M., Yin, S., Sun, C., Zhang, H., Zhuang, F., & Xi, J. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201203597 · Cited by 46 · see citing works
Transcription Activator-Like Effector Proteins Induce the Expression of the Frataxin Gene
Tremblay, J. P., Chapdelaine, P., Coulombe, Z., & Rousseau, J. (2012) · Human Gene Therapy · doi:10.1089/hum.2012.034 · Cited by 33 · see citing works
Flagged first, then by citations
1 of the 103 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696 · see citing works
CRISPR-Cas systems for editing, regulating and targeting genomes
Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153 · see citing works
Self-citationEfficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937 · see citing works
Dunbar, C. E., High, K. A., Joung, J. K., Kohn, D. B., Ozawa, K., & Sadelain, M. (2018) · Science · doi:10.1126/science.aan4672 · Cited by 1,410 · see citing works
Self-citationEfficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
Jao, L.-E., Wente, S. R., & Chen, W. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1308335110 · Cited by 1,261 · see citing works
Exploring treatment options in cancer: tumor treatment strategies
Liu, B., Zhou, H., Tan, L., Siu, K. T. H., & Guan, X.-Y. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01856-7 · Cited by 1,201 · see citing works
Delivering CRISPR: a review of the challenges and approaches
Lino, C. A., Harper, J. C., Carney, J. P., & Timlin, J. A. (2018) · Drug Delivery · doi:10.1080/10717544.2018.1474964 · Cited by 1,185 · see citing works
A guide to genome engineering with programmable nucleases
Kim, S., & Kim, J. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3686 · Cited by 1,162 · see citing works
CRISPR/Cas9 in Genome Editing and Beyond
Wang, H., Russa, M. F. L., & Qi, L. S. (2016) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060815-014607 · Cited by 1,165 · see citing works
Koike-Yusa, H., Li, Y., Tan, E.-P., Velasco-Herrera, M. D. C., & Yusa, K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2800 · Cited by 1,064 · see citing works
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
Komor, A. C., Badran, A. H., & Liu, D. R. (2016) · Cell · doi:10.1016/j.cell.2016.10.044 · Cited by 1,011 · see citing works
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
Tsai, S. Q., Wyvekens, N., Khayter, C., Foden, J. A., Thapar, V., Reyon, D., Goodwin, M. J., Aryee, M. J., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2908 · Cited by 952 · see citing works
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
Shen, B., Zhang, W., Zhang, J., Zhou, J., Wang, J.-Y., Chen, L., Wang, L., Hodgkins, A., Iyer, V., Huang, X., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2857 · Cited by 827 · see citing works
Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos
Chang, N., Sun, C., Gao, L., Zhu, D., Xu, X., Zhu, X., Xiong, J., & Xi, J. (2013) · Cell Research · doi:10.1038/cr.2013.45 · Cited by 819 · see citing works
Brooks, C., Nekrasov, V., Lippman, Z. B., & Eck, J. M. V. (2014) · PLANT PHYSIOLOGY · doi:10.1104/pp.114.247577 · Cited by 759 · see citing works
A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing
Mok, B. Y., de Moraes, M. H., Zeng, J., Bosch, D. E., Kotrys, A. V., Raguram, A., Hsu, F., Radey, M. C., Peterson, S. B., Mootha, V. K., et al. (2020) · Nature · doi:10.1038/s41586-020-2477-4 · Cited by 733 · see citing works
Bauer, D. E., Kamran, S. C., Lessard, S., Xu, J., Fujiwara, Y., Lin, C., Shao, Z., Canver, M. C., Smith, E. C., Pinello, L., et al. (2013) · Science · doi:10.1126/science.1242088 · Cited by 653 · see citing works
Hou, Z., Zhang, Y., Propson, N. E., Howden, S. E., Chu, L.-F., Sontheimer, E. J., & Thomson, J. A. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1313587110 · Cited by 681 · see citing works
Toruño, T. Y., Stergiopoulos, I., & Coaker, G. (2016) · Annual Review of Phytopathology · doi:10.1146/annurev-phyto-080615-100204 · Cited by 661 · see citing works
Off-target effects in CRISPR/Cas9 gene editing
Guo, C., Ma, X., Gao, F., & Guo, Y. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1143157 · Cited by 625 · see citing works
“Early studies utilize zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for genome editing (Urnov et al., 2010; Joung and Sander, 2013; Shamshirgaran et al., 2022).”
BackgroundPoirot, L., Philip, B., Schiffer-Mannioui, C., Le Clerre, D., Chion-Sotinel, I., Derniame, S., Potrel, P., Bas, C., Lemaire, L., Galetto, R., et al. (2015) · Cancer Research · doi:10.1158/0008-5472.can-14-3321 · Cited by 605 · see citing works
Delivery technologies for genome editing
Yin, H., Kauffman, K. J., & Anderson, D. G. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.280 · Cited by 597 · see citing works
Past, present, and future of CRISPR genome editing technologies
Pacesa, M., Pelea, O., & Jinek, M. (2024) · Cell · doi:10.1016/j.cell.2024.01.042 · Cited by 609 · see citing works
Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting
Aguirre, A. J., Meyers, R. M., Weir, B. A., Vázquez, F., Zhang, C., Ben‐David, U., Cook, A., Ha, G., Harrington, W. F., Doshi, M. B., et al. (2016) · Cancer Discovery · doi:10.1158/2159-8290.cd-16-0154 · Cited by 565 · see citing works
Targeted genome editing in human repopulating haematopoietic stem cells
Genovese, P., Schiroli, G., Escobar, G., Di Tomaso, T., Firrito, C., Calabria, A., Moi, D., Mazzieri, R., Bonini, C., Holmes, M. C., et al. (2014) · Nature · doi:10.1038/nature13420 · Cited by 555 · see citing works
Emerging evidence for functional peptides encoded by short open reading frames
Andrews, S. J., & Rothnagel, J. A. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3520 · Cited by 547 · see citing works
Genome Engineering with Targetable Nucleases
Carroll, D. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035418 · Cited by 541 · see citing works
Genetically modified crops: current status and future prospects
Kumar, K., Gambhir, G., Dass, A., Tripathi, A. K., Singh, A., Jha, A., Yadava, P., Choudhary, M., & Rakshit, S. (2020) · Planta · doi:10.1007/s00425-020-03372-8 · Cited by 546 · see citing works
Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR
Boettcher, M., & McManus, M. T. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.04.028 · Cited by 510 · see citing works
The 60-year evolution of lipid nanoparticles for nucleic acid delivery
Cullis, P. R., & Felgner, P. L. (2024) · Nature Reviews Drug Discovery · doi:10.1038/s41573-024-00977-6 · Cited by 517 · see citing works
Gonzãlez, F., Zhu, Z., Shi, Z., Lelli, K., Verma, N., Li, Q. V., & Huangfu, D. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.05.018 · Cited by 486 · see citing works
Maeder, M. L., Angstman, J. F., Richardson, M. E., Linder, S. J., Cascio, V. M., Tsai, S. Q., Ho, Q. V., Sander, J. D., Reyon, D., Bernstein, B., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2726 · Cited by 498 · see citing works
CRISPR-Cas: biology, mechanisms and relevance
Hille, F., & Charpentier, E. (2016) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0496 · Cited by 503 · see citing works
Efficient ablation of genes in human hematopoietic stem and effector cells using CRISPR/Cas9
Mandal, P. K., Ferreira, L. M. R., Collins, R., Meissner, T. B., Boutwell, C. L., Friesen, M., Vrbanac, V., Garrison, B. S., Stortchevoi, A., Bryder, D., et al. (2014) · Cell stem cell · doi:10.1016/j.stem.2014.10.004 · Cited by 475 · see citing works
Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish
Hruscha, A., Krawitz, P. M., Rechenberg, A., Heinrich, V., Hecht, J., Haass, C., & Schmid, B. (2013) · Development · doi:10.1242/dev.099085 · Cited by 466 · see citing works
Base editing: advances and therapeutic opportunities
Porto, E. M., Komor, A. C., Slaymaker, I. M., & Yeo, G. W.-M. (2020) · Nature Reviews Drug Discovery · doi:10.1038/s41573-020-0084-6 · Cited by 475 · see citing works
Ousterout, D. G., Kabadi, A. M., Thakore, P. I., Majoros, W. H., Reddy, T. E., & Gersbach, C. A. (2015) · Nature Communications · doi:10.1038/ncomms7244 · Cited by 470 · see citing works
Applications of the CRISPR-Cas9 system in cancer biology
Sánchez-Rivera, F. J., & Jacks, T. (2015) · Nature Reviews Cancer · doi:10.1038/nrc3950 · Cited by 405 · see citing works
Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Kaini, P., Sander, J. D., Joung, J. K., Peterson, R. T., & Yeh, J.-R. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0068708 · Cited by 367 · see citing works
Gene Editing and Crop Improvement Using CRISPR-Cas9 System
Arora, L., & Narula, A. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01932 · Cited by 399 · see citing works
Locus-specific editing of histone modifications at endogenous enhancers
Mendenhall, E. M., Williamson, K. E., Reyon, D., Zou, J., Ram, O., Joung, J. K., & Bernstein, B. (2013) · Nature Biotechnology · doi:10.1038/nbt.2701 · Cited by 362 · see citing works
Polymeric Delivery of Therapeutic Nucleic Acids
Kumar, R., Chalarca, C. F. S., Bockman, M. R., Bruggen, C. V., Grimme, C. J., Dalal, R. J., Hanson, M. G., Hexum, J. K., & Reineke, T. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00997 · Cited by 370 · see citing works
A synthetic sex ratio distortion system for the control of the human malaria mosquito
Galizi, R., Doyle, L., Menichelli, M., Bernardini, F., Dérédec, A., Burt, A., Stoddard, B. L., Windbichler, N., & Crisanti, A. (2014) · Nature Communications · doi:10.1038/ncomms4977 · Cited by 348 · see citing works
Oil palm natural diversity and the potential for yield improvement
Barcelos, E., Rios, S. D. A., da Cunha, R. N. V., Lopes, R., Motoike, S. Y., Babiychuk, E., Skirycz, A., & Kushnir, S. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00190 · Cited by 352 · see citing works
CRISPR/Cas9 for cancer research and therapy
Zhan, T., Rindtorff, N., Betge, J., Ebert, M., & Boutros, M. (2018) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2018.04.001 · Cited by 333 · see citing works
Feng, W., Newbigging, A. M., Tao, J., Cao, Y., Peng, H., Le, C., Wu, J., Pang, B., Li, J., Tyrrell, D. L., et al. (2021) · Chemical Science · doi:10.1039/d0sc06973f · Cited by 314 · see citing works
A new era in functional genomics screens
Przybyla, L., & Gilbert, L. A. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-021-00409-w · Cited by 281 · see citing works
Bacterial disease management: challenges, experience, innovation and future prospects
Sundin, G. W., Castiblanco, L. F., Yuan, X., Zeng, Q., & Yang, C. (2016) · Molecular Plant Pathology · doi:10.1111/mpp.12436 · Cited by 302 · see citing works
Sun, N., & Zhao, H. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.24890 · Cited by 301 · see citing works
Integrated omics analysis of specialized metabolism in medicinal plants
Rai, A., Saito, K., & Yamazaki, M. (2017) · The Plant Journal · doi:10.1111/tpj.13485 · Cited by 290 · see citing works
CRISPR-Cas9: A Preclinical and Clinical Perspective for the Treatment of Human Diseases
Sharma, G., Sharma, A. R., Bhattacharya, M., Lee, S., & Chakraborty, C. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.09.028 · Cited by 295 · see citing works
Simple and efficient CRISPR/Cas9‐mediated targeted mutagenesis in Xenopus tropicalis
Nakayama, T., Fish, M. B., Fisher, M. A., Oomen, J., Thomsen, G. H., & Grainger, R. M. (2013) · genesis · doi:10.1002/dvg.22720 · Cited by 283 · see citing works
CRISPR Crops: Plant Genome Editing Toward Disease Resistance
Langner, T., Kamoun, S., & Belhaj, K. (2018) · Annual Review of Phytopathology · doi:10.1146/annurev-phyto-080417-050158 · Cited by 290 · see citing works
Type II restriction endonucleases—a historical perspective and more
Pingoud, A. M., Wilson, G. G., & Wende, W. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku447 · Cited by 274 · see citing works
CRISPR/cas systems redefine nucleic acid detection: Principles and methods
Wang, M., Zhang, R., & Li, J. (2020) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2020.112430 · Cited by 272 · see citing works
Recent advances in engineering nonribosomal peptide assembly lines
Winn, M., Fyans, J. K., Zhuo, Y., & Micklefield, J. (2015) · Natural Product Reports · doi:10.1039/c5np00099h · Cited by 266 · see citing works
CRISPR technologies for genome, epigenome and transcriptome editing
Villiger, L., Joung, J., Koblan, L. W., Weissman, J. S., Abudayyeh, O. O., & Gootenberg, J. S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-023-00697-6 · Cited by 264 · see citing works
Advances in therapeutic CRISPR/Cas9 genome editing
Savić, N., & Schwank, G. (2016) · Translational Research · doi:10.1016/j.trsl.2015.09.008 · Cited by 257 · see citing works
Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery
Vaughan, H. J., Green, J. J., & Tzeng, S. Y. (2019) · Advanced Materials · doi:10.1002/adma.201901081 · Cited by 257 · see citing works
The Genome Landscape of the African Green Monkey Kidney-Derived Vero Cell Line
Osada, N., Kohara, A., Yamaji, T., Hirayama, N., Kasai, F., Sekizuka, T., Kuroda, M., & Hanada, K. (2014) · DNA Research · doi:10.1093/dnares/dsu029 · Cited by 250 · see citing works
Refining strategies to translate genome editing to the clinic
Cornu, T. I., Mussolino, C., & Cathomen, T. (2017) · Nature Medicine · doi:10.1038/nm.4313 · Cited by 247 · see citing works
Zhang, H., Qin, C., An, C., Zheng, X., Wen, S., Chen, W., Liu, X., Lv, Z., Yang, P., Xu, W., et al. (2021) · Molecular Cancer · doi:10.1186/s12943-021-01431-6 · Cited by 255 · see citing works
Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9
Zhang, H., Zhang, Y., & Yin, H. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.01.014 · Cited by 248 · see citing works
Hill, J. T., Demarest, B. L., Bisgrove, B. W., Su, Y., Smith, M., & Yost, H. J. (2014) · Developmental Dynamics · doi:10.1002/dvdy.24183 · Cited by 243 · see citing works
Bestrophin 1 and retinal disease
Johnson, A. A., Guziewicz, K. E., Lee, C. J., Kalathur, R., Pulido, J. S., Marmorstein, L. Y., & Marmorstein, A. D. (2017) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2017.01.006 · Cited by 241 · see citing works
Integrated approaches in microbial degradation of plastics
Jaiswal, S., Sharma, B., & Shukla, P. (2019) · Environmental Technology & Innovation · doi:10.1016/j.eti.2019.100567 · Cited by 241 · see citing works
Enhancer redundancy in development and disease
Kvon, E. Z., Waymack, R., Gad, M., & Wunderlich, Z. (2021) · Nature Reviews Genetics · doi:10.1038/s41576-020-00311-x · Cited by 226 · see citing works
Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
Gander, M. W., Vrana, J. D., Voje, W. E., Carothers, J. M., & Klavins, E. (2017) · Nature Communications · doi:10.1038/ncomms15459 · Cited by 237 · see citing works
Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava
Odipio, J., Alicai, T., Ingelbrecht, I., Nusinow, D. A., Bart, R. S., & Taylor, N. J. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01780 · Cited by 235 · see citing works
Towards social acceptance of plant breeding by genome editing
Araki, M., & Ishii, T. (2015) · Trends in Plant Science · doi:10.1016/j.tplants.2015.01.010 · Cited by 234 · see citing works
Zhen, S. M., Hua, L., Liu, Y.-H., Gao, L.-C., Fu, J., Wan, D.-Y., Dong, L.-H., Song, H.-F., & Gao, X. (2015) · Gene Therapy · doi:10.1038/gt.2015.2 · Cited by 233 · see citing works
Oceguera-Yañez, F., Kim, S., Matsumoto, T., Tan, G. W., Xiang, L., Hatani, T., Kondo, T., Ikeya, M., Yoshida, Y., Inoue, H., et al. (2015) · Methods · doi:10.1016/j.ymeth.2015.12.012 · Cited by 218 · see citing works
A CRISPR view of developmentPreprint
Harrison, M. M., Jenkins, B., O’Connor-Giles, K. M., & Wildonger, J. (2014) · Genes & Development · doi:10.1101/gad.248252.114 · Cited by 228 · see citing works
Targeted mutagenesis using CRISPR/Cas system in medaka
Ansai, S., & Kinoshita, M. (2014) · Biology Open · doi:10.1242/bio.20148177 · Cited by 222 · see citing works
Schneider, N., Sundaresan, Y., Gopalakrishnan, P., Beryozkin, A., Hanany, M., Levanon, E. Y., Banin, E., Ben‐Aroya, S., & Sharon, D. (2021) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2021.101029 · Cited by 225 · see citing works
Genetic engineering of microalgae for enhanced biorefinery capabilities
Fayyaz, M., Chew, K. W., Show, P. L., Ling, T. C., Ng, I.-S., & Chang, J.-S. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107554 · Cited by 225 · see citing works
Common marmoset as a new model animal for neuroscience research and genome editing technology
Kishi, N., Sato, K., Sasaki, E., & Okano, H. (2014) · Development Growth & Differentiation · doi:10.1111/dgd.12109 · Cited by 223 · see citing works
CAR T-cell therapy for B-cell lymphomas: clinical trial results of available products
Chávez, J. C., Bachmeier, C. A., & Kharfan‐Dabaja, M. A. (2019) · Therapeutic Advances in Hematology · doi:10.1177/2040620719841581 · Cited by 223 · see citing works
Mao, Y., Zhang, Z., Feng, Z., Wei, P., Zhang, H., Botella, J. R., & Zhu, J. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12468 · Cited by 217 · see citing works
Bloom, K., Ely, A., Mussolino, C., Cathomen, T., & Arbuthnot, P. (2013) · Molecular Therapy · doi:10.1038/mt.2013.170 · Cited by 217 · see citing works
Plant Genome Engineering for Targeted Improvement of Crop Traits
Sedeek, K. E. M., Mahas, A., & Mahfouz, M. M. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00114 · Cited by 232 · see citing works
Application of CRISPR/Cas9 in Crop Quality Improvement
Liu, Q., Yang, F., Zhang, J., Liu, H., Rahman, S., Islam, S., Ma, W., & She, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22084206 · Cited by 231 · see citing works
Evolution of transcription factor binding in metazoans — mechanisms and functional implications
Villar, D., Flicek, P., & Odom, D. T. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3481 · Cited by 206 · see citing works
CRISPR-Cas: New Tools for Genetic Manipulations from Bacterial Immunity Systems
Jiang, W., & Marraffini, L. A. (2015) · Annual Review of Microbiology · doi:10.1146/annurev-micro-091014-104441 · Cited by 210 · see citing works
Arazoe, T., Miyoshi, K., Yamato, T., Ogawa, T., Ohsato, S., Arie, T., & Kuwata, S. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25662 · Cited by 205 · see citing works
Chronic Granulomatous Disease: a Comprehensive Review
Yu, H.-H., Yang, Y.-H., & Chiang, B.-L. (2021) · Clinical Reviews in Allergy & Immunology · doi:10.1007/s12016-020-08800-x · Cited by 197 · see citing works
Zebrafish models in translational research: tipping the scales toward advancements in human health
Phillips, J. B., & Westerfield, M. (2014) · Disease Models & Mechanisms · doi:10.1242/dmm.015545 · Cited by 197 · see citing works
Wahane, A. V., Waghmode, A., Kapphahn, A., Dhuri, K., Gupta, A., & Bahal, R. (2020) · Molecules · doi:10.3390/molecules25122866 · Cited by 196 · see citing works
Debbarma, J., Sarki, Y. N., Saikia, B., Boruah, H. P. D., Singha, D. L., & Chikkaputtaiah, C. (2019) · Molecular Biotechnology · doi:10.1007/s12033-018-0144-x · Cited by 195 · see citing works
Gene editing and CRISPR in the clinic: current and future perspectives
Hirakawa, M. P., Krishnakumar, R., Timlin, J. A., Carney, J. P., & Butler, K. S. (2020) · Bioscience Reports · doi:10.1042/bsr20200127 · Cited by 197 · see citing works
Curtin, S. J., Xiong, Y., Michno, J., Campbell, B. W., Stec, A. O., Čermák, T., Starker, C. G., Voytas, D. F., Eamens, A. L., & Stupar, R. M. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12857 · Cited by 192 · see citing works
DNA methylation in social insects: how epigenetics can control behavior and longevity
Yan, H., Bonasio, R., Simola, D. F., Liebig, J., Berger, S. L., & Reinberg, D. (2015) · Annual Review of Entomology · doi:10.1146/annurev-ento-010814-020803 · Cited by 183 · see citing works
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity
Mussolino, C., Alzubi, J., Fine, E. J., Morbitzer, R., Cradick, T. J., Lahaye, T., Bao, G., & Cathomen, T. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku305 · Cited by 190 · see citing works
Chromothriptic Cure of WHIM Syndrome
McDermott, D. H., Gao, J., Liu, Q., Siwicki, M. R., Martens, C., Jacobs, P., Velez, D. S., Yim, E., Bryke, C. R., Hsu, N. Y.-H., et al. (2015) · Cell · doi:10.1016/j.cell.2015.01.014 · Cited by 190 · see citing works
The importance of genomic variation for biodiversity, ecosystems and people
Stange, M., Barrett, R. D. H., & Hendry, A. P. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-020-00288-7 · Cited by 186 · see citing works
Thomazella, D. P. D. T., Brail, Q., Dahlbeck, D., & Staskawicz, B. J. (2016) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/064824 · Cited by 192 · see citing works
Gene-based therapies for neurodegenerative diseases
Sun, J., & Roy, S. (2021) · Nature Neuroscience · doi:10.1038/s41593-020-00778-1 · Cited by 189 · see citing works
Targeted genome modification technologies and their applications in crop improvements
Chen, K., & Gao, C. (2013) · Plant Cell Reports · doi:10.1007/s00299-013-1539-6 · Cited by 187 · see citing works
Recent advances in the heterologous expression of microbial natural product biosynthetic pathways
Ongley, S. E., Bian, X., Neilan, B. A., & Müller, R. (2013) · Natural Product Reports · doi:10.1039/c3np70034h · Cited by 187 · see citing works
Marker-free coselection for CRISPR-driven genome editing in human cells
Agudelo, D., Duringer, A., Bozoyan, L., Huard, C., Carter, S., Loehr, J., Synodinou, D., Drouin, M., Salsman, J., Dellaire, G., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4265 · Cited by 174 · see citing works
- Authors
- Joung, J. K., & Sander, J. D.
- Affiliations
- Harvard University (US)
- Corresponding author
- Joung, J. Keith
- Venue
- Nature Reviews Molecular Cell Biology
- Published
- 2012-11-21
- Volume
- 14
- Issue
- 1
- Pages
- 49-55
- ISSN
- 1471-0072 (ISSN-L)
- 1471-0080
- PMC
- PMC3547402
- OpenAlex
- W2061184966
- Cited by
- 1,650
- References
- 103
- Citations by year
- 2026: 65 · 2025: 106 · 2024: 128 · 2023: 119 · 2022: 123 · 2021: 102 · 2020: 112 · 2019: 122 · 2018: 114 · 2017: 103 · 2016: 114 · 2015: 159 · 2014: 175 · 2013: 107
- FWCI
- 12.87
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nature Reviews Molecular Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Human Genome Research Institute (P50HG005550)
- National Cancer Institute (T32CA009216)
- National Institute of General Medical Sciences (DP1GM105378, R01GM088040)
- National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- TALEN, targeted genome editing, engineered nucleases, genetic modification, genetic disease treatment
- MeSH terms
- Animals, Deoxyribonucleases, DNA, DNA-Binding Proteins, Humans, Protein Engineering, Transcriptional Activation, Genome, Protein Structure, Tertiary
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
