Plückthun, Andreas

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides 1 1Edited by I. A. Wilson

Knappik, A., Ge, L., Honegger, A., Pack, P., Fischer, M., Wellnhofer, G., Hoess, A., Wölle, J., Plückthun, A., & Virnekäs, B. (2000) · Journal of Molecular Biology

Monoclonal and Polyclonal Antibodies ResearchChemical Synthesis and Analysis

Cited by 728

High-affinity binders selected from designed ankyrin repeat protein libraries

Binz, H. K., Amstutz, P., Kohl, A., Stumpp, M. T., Briand, C., Forrer, P., Grütter, M. G., & Plückthun, A. (2004) · Nature Biotechnology

Monoclonal and Polyclonal Antibodies ResearchBiochemical and Structural Characterization

Cited by 656

Reproducibility: Standardize antibodies used in research

Bradbury, A., & Plückthun, A. (2015) · Nature

Monoclonal and Polyclonal Antibodies ResearchAnimal testing and alternatives

Cited by 647

Stability engineering of antibody single-chain Fv fragments

Wörn, A., & Plückthun, A. (2001) · Journal of Molecular Biology

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 616

Designing Repeat Proteins: Well-expressed, Soluble and Stable Proteins from Combinatorial Libraries of Consensus Ankyrin Repeat Proteins

Binz, H. K., Stumpp, M. T., Forrer, P., Amstutz, P., & Plückthun, A. (2003) · Journal of Molecular Biology

Protein Structure and DynamicsBiochemical and Structural Characterization

Cited by 589

Domain interactions in the Fab fragment: A comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability

Röthlisberger, D., Honegger, A., & Plückthun, A. (2005) · Journal of Molecular Biology

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 289

PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling

Yen, H.-Y., Hoi, K. K., Liko, I., Hedger, G., Horrell, M. R., Song, W., Wu, D., Heine, P., Warne, T., Lee, Y., et al. (2018) · Nature

Receptor Mechanisms and SignalingProtein Kinase Regulation and GTPase Signaling

Cited by 284

Covalently circularized nanodiscs for studying membrane proteins and viral entry

Nasr, M. L., Baptista, D., Strauss, M., Sun, Z.-Y. J., Grigoriu, S., Huser, S., Plückthun, A., Hagn, F., Walz, T., Hogle, J. M., et al. (2017) · Nature Methods

Lipid Membrane Structure and BehaviorProtein Structure and Dynamics

Cited by 262

The Periplasmic Escherichia coli Peptidylprolyl cis,trans-Isomerase FkpA

Bothmann, H., & Plückthun, A. (2000) · Journal of Biological Chemistry

Heat shock proteins researchProtein purification and stability

Cited by 192

Animal‐free alternatives and the antibody iceberg

Gray, A., Bradbury, A. R. M., Knappik, A., Plückthun, A., Borrebaeck, C. A. K., & Dübel, S. (2020) · Nature Biotechnology

Monoclonal and Polyclonal Antibodies ResearchAnimal testing and alternatives

Cited by 111

Crystal structure of the human oxytocin receptor

Waltenspühl, Y., Schöppe, J., Ehrenmann, J., Kummer, L., & Plückthun, A. (2020) · Science Advances

Receptor Mechanisms and SignalingNeuroendocrine regulation and behavior

Cited by 95

Removal of the conserved disulfide bridges from the scFv fragment of an antibody: Effects on folding kinetics and aggregation

Ramm, K., Gehrig, P., & Plückthun, A. (1999) · Journal of Molecular Biology

Protein Structure and DynamicsProtein purification and stability

Cited by 55

The rate-limiting steps for the folding of an antibody scFv fragment

Jäger, M., & Plückthun, A. (1997) · FEBS Letters

Protein Structure and DynamicsProtein purification and stability

Cited by 44

Animal- versus in vitro-derived antibodies: avoiding the extremes

Bradbury, A. R. M., Dübel, S., Knappik, A., & Plückthun, A. (2021) · mAbs

Monoclonal and Polyclonal Antibodies ResearchAnimal testing and alternatives

Cited by 13

Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the Helicobacter pylori type IV secretion system

Blanc, M., Lettl, C., Guérin, J., Vieille, A., Furler, S., Briand-Schumacher, S., Dreier, B., Bergé, C., Plückthun, A., Vadon-Le Goff, S., et al. (2023) · PLOS Pathogens

Helicobacter pylori-related gastroenterology studiesBiochemical and Structural Characterization

Cited by 9

Selective targeting of TBXT with DARPins identifies regulatory networks and therapeutic vulnerabilities in chordoma

Umbaugh, C. S., Groth, M., Erkut, C., Lee, K.-S., Marinho, J., Linder, S., Iser, F., Kapp, J. N., Schroeter, P., Dolaner, S., et al. (2025) · Science Advances

Bone Tumor Diagnosis and TreatmentsProtein Degradation and Inhibitors

Cited by 6

Development of LRRK2 designed ankyrin-repeat proteins

Dederer, V., Murillo, M. S., Karasmanis, E. P., Hatch, K. S., Chatterjee, D., Preuss, F., Abdul Azeez, K. R., Nguyen, L. V., Galicia, C., Dreier, B., et al. (2023) · Journal of Biological Chemistry

Parkinson's Disease Mechanisms and TreatmentsProtein Kinase Regulation and GTPase Signaling

Cited by 5