Manometric Techniques and Related Methods for the Study of Tissue Metabolism.

Abstract

This book is intended for the beginning graduate student. It contains detailed descriptions of apparatus and technics for methods which have been found to be satisfactory in the laboratories of the authors and of their associates at the University of Wisconsin. It is not intended to be comprehensive but includes only methods which require a minimum of equipment. The Warburg respirometer and the differential manometer are described in detail, with typical examples of their use and of the calculations involved. In addition, there are descriptions of Thunberg technics and electrometric methods for the determination of dissolved oxygen and for the determination of oxidation-reduction potentials. The chapters "Methods of Tissue Preparation," "Manometric Estimation of Metabolites and Enzyme Systems," "Methods for the Analysis of Phosphorylated Intermediates" and "Preparation of Physiologically Important Intermediates and Metabolites" should be particularly valuable for the beginner in this field. There are some inexact and ambiguous statements and