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Trypan Blue Exclusion Test of Cell Viability
Abstract
The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.
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Research works printed but not linked: 1
- H.M. Shapiro 1988 John Wiley & Sons New York 129
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Metadata
- Authors
- Strober, W.
- Venue
- Current Protocols in Immunology
- Publisher
- Wiley
- Published
- 1997-03
- Volume
- 21
- Issue
- 1
- Pages
- Appendix 3B-Appendix 3B
- ISSN
- 1934-3671 (ISSN-L)
- 1934-368X
- OpenAlex
- W4230982340
- Cited by
- 1,345
- References
- 0
- FWCI
- 0.23
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
