Chemically induced mouse models of acute and chronic intestinal inflammation
Abstract
Inflammatory bowel diseases (IBDs) result in diarrhea and abdominal pain with further potential complications such as tissue fibrosis and stenosis. Animal models help in understanding the immunopathogenesis of IBDs and in the design of novel therapeutic concepts. Here we present an updated version of a protocol we published in 2007 for key models of acute and chronic forms of colitis induced by 2,4,6-trinitro-benzene sulfonic acid (TNBS), oxazolone and dextran sulfate sodium (DSS). This protocol update describes an adaptation of the existing protocol that modifies the technique. This protocol has been used to generate improved mouse models that better reflect the nature of IBDs in humans. In TNBS and oxazolone colitis models, topical administration of hapten reagents results in T-cell-mediated immunity against haptenized proteins and luminal antigens. By contrast, to generate DSS colitis models, mice orally receive DSS, causing death of epithelial cells, compromising barrier function and causing subsequent inflammation. The analysis of the acute colitis models can be performed within 1-2 weeks, whereas that of the chronic models may take 2-4 months. The strengths of the acute models are that they are based on the analysis of short-lasting barrier alterations, innate immune effects and flares. The advantages of the chronic models are that they may offer better insight into adaptive immunity and complications such as neoplasia and tissue fibrosis. The protocol requires basic sk…
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Colitis severity was assessed using the established Murine Endoscopic Index of Colitis Severity (MEICS) scoring system based on five parameters.
“Murine Endoscopic Index of Colitis Severity [MEICS] scores for colitis severity were assigned at the end of the experiment on the basis of five parameters [translucency, granularity, fibrin, vascularity, and stool] according to an established scoring system 29–31.”
From PU.1-driven Th9 Cells Promote Colorectal Cancer in Experimental Colitis Models Through Il-6 Effects in Intestinal Epithelial Cells (2022) · cites this paper as [31]
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- Authors
- Wirtz, S., Popp, V., Kindermann, M., Gerlach, K., Weigmann, B., Fichtner-Feigl, S., & Neurath, M. F.
- Affiliations
- Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
- Corresponding author
- Neurath, Markus F
- Venue
- Nature Protocols
- Published
- 2017-06-01
- Volume
- 12
- Issue
- 7
- Pages
- 1295-1309
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2620756707
- Cited by
- 1,575
- References
- 1
- Citations by year
- 2026: 222 · 2025: 234 · 2024: 266 · 2023: 214 · 2022: 204 · 2021: 171 · 2020: 121 · 2019: 92 · 2018: 42 · 2017: 8
- FWCI
- 47.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Health Sciences › Medicine › Ophthalmology › Ocular Diseases and Behçet’s Syndrome
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- inflammatory bowel disease, chemically induced colitis, mouse model, TNBS-induced colitis, DSS-induced colitis, acute colitis, chronic colitis, intestinal barrier dysfunction, T cell immunity, epithelial cell death, fibrosis, cancer
- MeSH terms
- Animals, Chronic Disease, Colitis, Disease Models, Animal, Oxazolone, Trinitrobenzenesulfonic Acid, Dextran Sulfate, Mice
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