Flow Rate Calibration for Absolute Cell Counting Rationale and Design
Abstract
There is a need for absolute leukocyte enumeration in the clinical setting, and accurate, reliable (and affordable) technology to determine absolute leukocyte counts has been developed. Such technology includes single platform and dual platform approaches. Derivations of these counts commonly incorporate the addition of a known number of latex microsphere beads to a blood sample, although it has been suggested that the addition of beads to a sample may only be required to act as an internal quality control procedure for assessing the pipetting error. This unit provides the technical details for undertaking flow rate calibration that obviates the need to add reference beads to each sample. It is envisaged that this report will provide the basis for subsequent clinical evaluations of this novel approach.
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Differences in immune reactivity and susceptibility to infectious diseases between males and females are well described.
“Differences in immune reactivity and susceptibility to infectious diseases between males and females is well described (reviewed in [40], [41], [46], [47], [48]).”
From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [41]
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A Cellular Cytotoxicity Assay using Ready-to-Thaw Target Cells without Washing StepsPreprint
Storm, J., Kusch, N., Güttler, M., Fode, C., Breuer, L., Bartling, J., Knabbe, C., Kaltschmidt, B., & Kaltschmidt, C. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.21.700863
- Authors
- Walker, C., & Barnett, D.
- Affiliations
- Royal Hallamshire Hospital (GB)
- Venue
- Current Protocols in Cytometry
- Publisher
- Wiley
- Published
- 2006-04
- Volume
- 36
- Issue
- 1
- Pages
- Unit6.24-Unit6.24
- ISSN
- 1934-9297 (ISSN-L)
- 1934-9300
- OpenAlex
- W1557724356
- Cited by
- 5
- References
- 13
- Citations by year
- 2026: 1 · 2015: 2 · 2014: 2
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Protocols in Cytometry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Health Sciences › Health Professions › Medical Laboratory Technology › Quality and Safety in Healthcare
- Keywords
- flow calibration, pipetting errors, internal quality control, blood sample preparation
- MeSH terms
- Blood Cells, Calibration, Flow Cytometry, Humans, Leukocyte Count, Leukocytes, Research Design, Sensitivity and Specificity, Reproducibility of Results
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