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Structural basis of a GatC ortholog transporter in the bacterial phosphotransferase system
Open accessPublished version of a preprint
Abstract
The bacterial phosphotransferase system (PTS) mediates the uptake of specific carbohydrates via IIC transporters. Here, we report the crystal and cryo‐electron microscopy (cryo‐EM) structures of Leminorella grimontii galactitol‐specific PTS enzyme IIC component (LgGatC), which is implicated in D‐xylose uptake and belongs to the ascorbate–galactitol (AG) superfamily of IIC proteins. These structures, determined in the presence and absence of D‐xylose, capture the transporter in an outward‐facing conformation. A homology model of an inward‐facing state, constructed based on these structures, supports an elevator‐like transport mechanism. These findings provide structural insights into substrate recognition by GatC and offer a framework for understanding sugar transport in PTS IIC proteins.
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Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
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Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., et al. (2024) · Nature · doi:10.1038/s41586-024-07487-w · Cited by 15,861
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Metadata
- Authors
- Takahashi, Y. S., Kohga, H., Chek, M. F., Yamamoto, K., Takahashi, J. F., Shigematsu, H., Tanaka, Y., Ichikawa, M., Miyazaki, R., Hakoshima, T., & Tsukazaki, T.
- Affiliations
- Nara Institute of Science and Technology (JP)
- Japan Synchrotron Radiation Research Institute (JP)
- Fudan University (CN)
- Corresponding author
- Tsukazaki, Tomoya
- ORCID
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2025-08
- Volume
- 599
- Issue
- 16
- Pages
- 2377-2387
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W4413002686
- Cited by
- 1
- References
- 2
- Citations by year
- 2026: 1
- FWCI
- 0.21
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- FEBS Letters (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (acceptedVersion)
- Funders
- Natural Science Foundation of Shanghai Municipality (24ZR1403800)
- Takeda Science Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- galactitol, transporter conformation, substrate recognition, sugar transport, homology model
- MeSH terms
- Bacterial Proteins, Models, Molecular, Phosphoenolpyruvate Sugar Phosphotransferase System, Protein Conformation, Xylose, Crystallography, X-Ray, Cryoelectron Microscopy, Planctomycetales
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