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Cited by 469

The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.

Circular Dichroism and Linear Dichroism

Rodger, Alison·2014·Encyclopedia of Analytical Chemistry

Abstract

Circular dichroism (CD) is the difference in absorption, A, of left and right circularly polarized light, Equation (1): For randomly oriented systems such as solutions of molecules, only chiral molecules will show any CD intensity corresponding to their absorption bands. Chiral molecules are those molecules that cannot be superposed on their mirror images. Chiral is derived from the Greek word χϵιρ meaning hand, hence the alternate term for chirality, ‘handedness’. Two molecules that are mirror images of each other are often referred to as enantiomers, and equimolar mixtures of two enatiomers form a racemic mixture, which has no net CD intensity in solution. CD can be used to analyze chiral structures such as protein secondary structure in molecules and to probe interactions between chiral molecules and other molecules. Linear dichroism (LD) is the difference in absorption of light linearly polarized parallel and perpendicular to an orientation axis, Equation (2): LD can be used to provide orientation information about subunits of a molecular system such as small molecules absorbed onto stretched films, flow‐oriented DNAs and fibrous proteins, and lipid bilayer systems.

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Metadata

Authors
Rodger, A.
Venue
Encyclopedia of Analytical Chemistry
Publisher
Wiley
Published
2014-01-13
Pages
1-34
OpenAlex
W2172047227
Cited by
469
References
22
Type
reference entry
Language
en
Open access
closed

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Rodger, A. (2014). Circular Dichroism and Linear Dichroism. Encyclopedia of Analytical Chemistry. https://doi.org/10.1002/9780470027318.a5402.pub2

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