The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Copper‐Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
Open accessAbstract
The copper‐catalyzed azide‐alkyne cycloaddition reaction is widely used for the connection of molecular entities of all sizes. A protocol is provided here for the process with biomolecules. Ascorbate is used as reducing agent to maintain the required cuprous oxidation state. Since these convenient conditions produce reactive oxygen species, five equivalents of a copper‐binding ligand are used with respect to metal. The ligand both accelerates the reaction and serves as a sacrificial reductant, protecting the biomolecules from oxidation. A procedure is also described for testing the efficiency of the reaction under desired conditions for purposes of optimization, before expensive biological reagents are used. Curr. Protoc. Chem. Biol. 3:153‐162 © 2011 by John Wiley & Sons, Inc.
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1,3‐Dipolar Cycloadditions. Past and Future
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Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
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Self-citationPolytriazoles as Copper(I)-Stabilizing Ligands in Catalysis
Chan, T. R., Hilgraf, R., Sharpless, K. B., & Fokin, V. V. (2004) · Organic Letters · doi:10.1021/ol0493094 · Cited by 1,531
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Analysis and Optimization of Copper‐Catalyzed Azide–Alkyne Cycloaddition for Bioconjugation
Hong, V., Presolski, S. I., Ma, C., & Finn, M. G. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200905087 · Cited by 1,029
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Chemical remodelling of cell surfaces in living animals
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Metadata
- Authors
- Presolski, S. I., Hong, V. P., & Finn, M. G.
- Venue
- Current Protocols in Chemical Biology
- Publisher
- Wiley
- Published
- 2011-12
- Volume
- 3
- Issue
- 4
- Pages
- 153-162
- ISSN
- 2160-4762 (ISSN-L)
- OpenAlex
- W1951491916
- Cited by
- 416
- References
- 41
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- green
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