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Monodisperse Alginate Hydrogel Microbeads for Cell Encapsulation
Abstract
A method that combines internal gelation with T-junction droplet formation in microfluidic devices for the production of monodisperse alginate hydrogel microbeads is described. Our approach gives high uniformity, and allows better control over both size and shape of the microbeads compared to conventional external gelation methods performed in microfluidic devices (see figure). Also, we demonstrate that our approach is mild enough to encapsulate mammalian cells (Jurkat) without loss of their viability.
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Cited by
Biofabrication strategies for 3D in vitro models and regenerative medicine
Moroni, L., Burdick, J. A., Highley, C., Lee, S. J., Morimoto, Y., Takeuchi, S., & Yoo, J. J. (2018) · Nature Reviews Materials · doi:10.1038/s41578-018-0006-y · Cited by 753
Self-citationDesigning degradable hydrogels for orthogonal control of cell microenvironments
Kharkar, P. M., Kiick, K. L., & Kloxin, A. M. (2013) · Chem. Soc. Rev. · doi:10.1039/c3cs60040h · Cited by 717
Sabhachandani, P., Motwani, V., Cohen, N., Sarkar, S., Torchilin, V., & Konry, T. (2016) · Lab on a Chip · doi:10.1039/c5lc01139f · Cited by 193
Karamikamkar, S., Yalcintas, E. P., Haghniaz, R., de Barros, N. R., Mecwan, M., Nasiri, R., Davoodi, E., Nasrollahi, F., Erdem, A., Kang, H., et al. (2023) · Advanced Science · doi:10.1002/advs.202204681 · Cited by 109
Scott, E. A., Nichols, M. D., Kuntz-Willits, R., & Elbert, D. L. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.07.009 · Cited by 99
Zhang, R., Lei, L., Song, Q., & Li, X. (2019) · Colloids and Surfaces B: Biointerfaces · doi:10.1016/j.colsurfb.2018.11.083 · Cited by 89
Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment
Mirdamadi, E. S., Kalhori, D., Zakeri, N., Azarpira, N., & Solati-Hashjin, M. (2020) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2019.0233 · Cited by 57
Zhu, Y., Li, J., Lin, X., Huang, X., & Hoffmann, M. R. (2021) · ACS Applied Bio Materials · doi:10.1021/acsabm.0c01615 · Cited by 18
Metadata
- Authors
- Tan, W. ‐., & Takeuchi, S.
- Venue
- Advanced Materials
- Publisher
- Wiley
- Published
- 2007-08-22
- Volume
- 19
- Issue
- 18
- Pages
- 2696-2701
- ISSN
- 0935-9648 (ISSN-L)
- 1521-4095
- OpenAlex
- W2028253963
- Cited by
- 599
- References
- 0
- FWCI
- 14.86
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
