The Alzheimer's Association international guidelines for handling of cerebrospinal fluid for routine clinical measurements of amyloid β and tau
Open accessAbstract
The core cerebrospinal fluid (CSF) Alzheimer's disease (AD) biomarkers amyloid beta (Aβ42 and Aβ40), total tau, and phosphorylated tau, have been extensively clinically validated, with very high diagnostic performance for AD, including the early phases of the disease. However, between‐center differences in pre‐analytical procedures may contribute to variability in measurements across laboratories. To resolve this issue, a workgroup was led by the Alzheimer's Association with experts from both academia and industry. The aim of the group was to develop a simplified and standardized pre‐analytical protocol for CSF collection and handling before analysis for routine clinical use, and ultimately to ensure high diagnostic performance and minimize patient misclassification rates. Widespread application of the protocol would help minimize variability in measurements, which would facilitate the implementation of unified cut‐off levels across laboratories, and foster the use of CSF biomarkers in AD diagnostics for the benefit of the patients.
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CSF samples were collected and handled according to current international recommendations.
“CSF samples were collected and handled according to current international recommendations44,62.”
From Highly accurate blood test for Alzheimer's disease is similar or superior to clinical cerebrospinal fluid tests (2024) · cites this paper as [62]
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Biomarkers for neurodegenerative diseases
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Self-citation
- Authors
- Hansson, O., Batrla, R., Brix, B., Carrillo, M. C., Corradini, V., Edelmayer, R. M., Esquivel, R. N., Hall, C., Lawson, J., Bastard, N. L., Molinuevo, J. L., Nisenbaum, L. K., Rutz, S., Salamone, S. J., Teunissen, C. E., Traynham, C., Umek, R. M., Vanderstichele, H., Vandijck, M., Wahl, S., Weber, C. J., Zetterberg, H., & Blennow, K.
- Affiliations
- Lund University (SE)
- Novartis (Switzerland) (CH)
- Euroimmun Medizinische Labordiagnostika (Germany) (DE)
- Alzheimer's Association Chicago Illinois USA
- F. Hoffmann-La Roche AG (CH)
- Alzheimer's Association (US)
- Fujirebio US (United States) (US)
- Fujirebio Diagnostics AB Göteborg Sweden
- Fujirebio (Belgium) (BE)
- BarcelonaBeta Brain Research Center Pasqual Maragall Foundation Barcelona Barcelona Spain
- Biogen (United States) (US)
- Roche Diagnostics GmbH Penzberg Germany
- Saladax Biomedical (United States) (US)
- Amsterdam Neuroscience (NL)
- Meso Scale Diagnostics Rockville Maryland USA
- Biomarkable bv Consulting Ghent Belgium
- Sahlgrenska University Hospital (SE)
- Corresponding author
- Weber, Christopher J.
- ORCID
- Venue
- Alzheimer's & Dementia
- Publisher
- Wiley
- Published
- 2021-03-31
- Volume
- 17
- Issue
- 9
- Pages
- 1575-1582
- ISSN
- 1552-5260 (ISSN-L)
- 1552-5279
- OpenAlex
- W3145486919
- Cited by
- 136
- References
- 14
- Citations by year
- 2026: 29 · 2025: 28 · 2024: 35 · 2023: 22 · 2022: 16 · 2021: 6
- FWCI
- 14.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Alzheimer s & Dementia (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion, other-oa)
- Pure Amsterdam UMC (acceptedVersion, cc-by)
- UCL Discovery (University College London) (submittedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Funders
- Alzheimer's Association
- Australian Government
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet (2017-00915)
- H2020 European Research Council (681712)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- cerebrospinal fluid, Alzheimer's disease
- MeSH terms
- Alzheimer Disease, Humans, Phosphorylation, Specimen Handling, Biomarkers, Amyloid beta-Peptides, tau Proteins, Guidelines as Topic, Clinical Laboratory Techniques, Internationality
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