Signal Amplification by Allosteric Catalysis
Abstract
In this article we unify a series of recent studies on bio‐ and chemosensors under a single signaling strategy: signal amplification by allosteric catalysis (SAAC). The SAAC strategy mimics biological signal transduction processes, where molecular recognition between an external signal and a protein receptor is allosterically transduced into catalytically amplified chemical information (usually second messengers). Several recent biosensing and chemosensing studies apply this nature‐inspired strategy by using engineered allosteric enzymes, ribozymes, or regulatable organic catalysts. The factors pertinent to achieving high sensitivity and specificity in SAAC strategies are analyzed. The authors believe that these early studies from a variety of research groups have opened up a new venue for the development of sensing technologies where molecular recognition and catalysis can be coupled for practical purposes.
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The combination of allosteric regulation with a catalytic output can amplify the response to the signal, ensuring it successfully reaches the nucleus.
“The combination of allosteric regulation with a catalytic output (e.g. kinase activity) can also amplify the response to the signal, ensuring it successfully reaches the nucleus [31].”
From Intrinsically disordered proteins play diverse roles in cell signaling (2022) · cites this paper as [31]
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- Authors
- Zhu, L., & Anslyn, E. V.
- Affiliations
- The University of Texas at Austin (US)
- Corresponding author
- Anslyn, Eric V.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2006-02-06
- Volume
- 45
- Issue
- 8
- Pages
- 1190-1196
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2042008342
- Cited by
- 151
- References
- 80
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 4 · 2021: 7 · 2020: 7 · 2019: 2 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 12 · 2014: 14 · 2013: 10 · 2012: 11
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Angewandte Chemie International Edition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (EB00549-5, R01EB000549)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Keywords
- biosensors, chemical sensors, signal transduction, ribozymes, molecular recognition, second messengers, sensitivity and specificity
- MeSH terms
- Allosteric Regulation, Catalysis, Nucleic Acid Conformation, Sensitivity and Specificity, Biosensing Techniques, Molecular Structure, Signal Transduction
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