Cited by 151Advanced biosensing and bioanalysis techniquesMolecular Sensors and Ion DetectionElectrochemical sensors and biosensors

Signal Amplification by Allosteric Catalysis

Zhu, Lei, Anslyn, Eric V.·2006·Angewandte Chemie International Edition

Abstract

In this article we unify a series of recent studies on bio‐ and chemosensors under a single signaling strategy: signal amplification by allosteric catalysis (SAAC). The SAAC strategy mimics biological signal transduction processes, where molecular recognition between an external signal and a protein receptor is allosterically transduced into catalytically amplified chemical information (usually second messengers). Several recent biosensing and chemosensing studies apply this nature‐inspired strategy by using engineered allosteric enzymes, ribozymes, or regulatable organic catalysts. The factors pertinent to achieving high sensitivity and specificity in SAAC strategies are analyzed. The authors believe that these early studies from a variety of research groups have opened up a new venue for the development of sensing technologies where molecular recognition and catalysis can be coupled for practical purposes.

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Crossref · 10.1002/anie.200501476

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  • The combination of allosteric regulation with a catalytic output can amplify the response to the signal, ensuring it successfully reaches the nucleus.

    “The combination of allosteric regulation with a catalytic output (e.g. kinase activity) can also amplify the response to the signal, ensuring it successfully reaches the nucleus [31].”

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Authors
Zhu, L., & Anslyn, E. V.
Affiliations
The University of Texas at Austin (US)
Corresponding author
Anslyn, Eric V.
Venue
Angewandte Chemie International Edition
Publisher
Wiley
Published
2006-02-06
Volume
45
Issue
8
Pages
1190-1196
ISSN
  • 1433-7851 (ISSN-L)
  • 1521-3773
OpenAlex
W2042008342
Cited by
151
References
80
Citations by year
2026: 2 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 4 · 2021: 7 · 2020: 7 · 2019: 2 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 12 · 2014: 14 · 2013: 10 · 2012: 11
FWCI
0.90
Type
journal article
Language
en
Open access
closed
Locations
  • Angewandte Chemie International Edition (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Biomedical Imaging and Bioengineering (EB00549-5, R01EB000549)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
Keywords
biosensors, chemical sensors, signal transduction, ribozymes, molecular recognition, second messengers, sensitivity and specificity
MeSH terms
Allosteric Regulation, Catalysis, Nucleic Acid Conformation, Sensitivity and Specificity, Biosensing Techniques, Molecular Structure, Signal Transduction

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