The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Droplet Microfluidics—A Tool for Single‐Cell Analysis
Abstract
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse picoliter liquid capsules and manipulations at a throughput of thousands of droplets per second. These qualities allow many of the challenges in single‐cell analysis to be overcome. Monodispersity enables quantitative control of solute concentrations, while encapsulation in droplets provides an isolated compartment for the single cell and its immediate environment. The high throughput allows the processing and analysis of the tens of thousands to millions of cells that must be analyzed to accurately describe a heterogeneous cell population so as to find rare cell types or access sufficient biological space to find hits in a directed evolution experiment. The low volumes of the droplets make very large screens economically viable. This Review gives an overview of the current state of single‐cell analysis involving droplet microfluidics and offers examples where droplet microfluidics can further biological understanding.
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The sequence of the human genome
Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) · Science · doi:10.1126/science.1058040 · Cited by 13,909
High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
Hindson, B. J., Ness, K. D., Masquelier, D. A., Belgrader, P., Heredia, N. J., Makarewicz, A. J., Bright, I. J., Lucero, M. Y., Hiddessen, A. L., Legler, T. C., et al. (2011) · Analytical Chemistry · doi:10.1021/ac202028g · Cited by 2,792
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
Newman, J. R. S., Ghaemmaghami, S., Ihmels, J., Breslow, D. K., Noble, M., DeRisi, J. L., & Weissman, J. S. (2006) · Nature · doi:10.1038/nature04785 · Cited by 1,483
Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents
Song, H., & Ismagilov, R. F. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0354566 · Cited by 693
Microdroplet-based PCR enrichment for large-scale targeted sequencing
Tewhey, R., Warner, J. B., Nakano, M., Libby, B., Medkova, M., David, P. H., Kotsopoulos, S. K., Samuels, M. L., Hutchison, J. B., Larson, J. W., et al. (2009) · Nature Biotechnology · doi:10.1038/nbt.1583 · Cited by 454
Amplification of complex gene libraries by emulsion PCR
Williams, R., Peisajovich, S. G., Miller, O. J., Magdassi, S., Tawfik, D. S., & Griffiths, A. D. (2006) · Nature Methods · doi:10.1038/nmeth896 · Cited by 358
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A global view of protein expression in human cells, tissues, and organs
Pontén, F., Gry, M., Fagerberg, L., Lundberg, E., Asplund, A., Berglund, L., Oksvold, P., Björling, E., Hober, S., Kampf, C., et al. (2009) · Molecular Systems Biology · doi:10.1038/msb.2009.93 · Cited by 222
Cited by
Magnetic digital microfluidics – a review
Zhang, Y., & Nguyen, N.-T. (2017) · Lab on a Chip · doi:10.1039/c7lc00025a · Cited by 364
Recent Advances in Droplet Microfluidics
Ding, Y., Howes, P. D., & deMello, A. J. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b05047 · Cited by 357
Digital Assays Part I: Partitioning Statistics and Digital PCR
Basu, A. S. (2017) · SLAS Technology · doi:10.1177/2472630317705680 · Cited by 300
Kang, D.-K., Ali, M. M., Zhang, K., Huang, S. S., Peterson, E., Digman, M. A., Gratton, E., & Zhao, W. (2014) · Nature Communications · doi:10.1038/ncomms6427 · Cited by 297
Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development
Dolega, M. E., Abeille, F., Picollet-D'hahan, N., & Gidrol, X. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.02.042 · Cited by 91
Metadata
- Authors
- Joensson, H. N., & Andersson Svahn, H.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2012-11-23
- Volume
- 51
- Issue
- 49
- Pages
- 12176-12192
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2106342149
- Cited by
- 557
- References
- 8
- FWCI
- 17.61
- Type
- journal article
- Language
- en
- Open access
- closed
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- Joensson, H. N., & Andersson Svahn, H. (2012). Droplet Microfluidics—A Tool for Single‐Cell Analysis. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201200460
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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