Amplification of complex gene libraries by emulsion PCR
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Emulsion PCR was used to reduce PCR bias and prevent chimeric PCR products.
“Emulsion polymerase chain reaction (PCR) was used to recover gRNA and UCB sequences from plasmid libraries, genomic DNA extracted from lentivirus-infected hES cells and cells sorted from CHOOSE mosaic organoids as well as 10× single-cell complementary DNA libraries to reduce PCR bias and to prevent the generation of chimeric PCR products56,57.”
From Single-cell brain organoid screening identifies developmental defects in autism (2023) · cites this paper as [57]
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Analysis of aptamer discovery and technology
Dunn, M. R., Jimenez, R. M., & Chaput, J. C. (2017) · Nature Reviews Chemistry · doi:10.1038/s41570-017-0076 · Cited by 938 · see citing works
Single-cell brain organoid screening identifies developmental defects in autism
Li, C., Fleck, J. S., Martins-Costa, C., Burkard, T. R., Themann, J., Stuempflen, M., Peer, A. M., Vertesy, Á., Littleboy, J. B., Esk, C., et al. (2023) · Nature · doi:10.1038/s41586-023-06473-y · Cited by 283 · see citing works
“Emulsion PCR and target amplification Emulsion PCR was used to recover gRNA and unique clone barcode (UCB) sequences from plasmid libraries, genomic DNA extracted from lentivirus infected hESCs, and 10X single cell cDNA libraries to reduce PCR bias and to prevent the generation of chimeric PCR products (54, 55).”
MethodDavidsson, M., Wang, G., Aldrin-Kirk, P., Cardoso, T., Nolbrant, S., Hartnor, M., Mudannayake, J., Parmar, M., & Björklund, T. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1910061116 · Cited by 142 · see citing works
Choi, Y., Ryu, T., Lee, A. C., Choi, H., Lee, H., Park, J., Song, S.-H., Kim, S., Kim, H., Park, W., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-43105-w · Cited by 128 · see citing works
In situ genotyping of a pooled strain library after characterizing complex phenotypes
Lawson, M. J., Camsund, D., Larsson, J., Baltekin, Ö., Fange, D., & Elf, J. (2017) · Molecular Systems Biology · doi:10.15252/msb.20177951 · Cited by 82 · see citing works
- Authors
- Williams, R., Peisajovich, S. G., Miller, O. J., Magdassi, S., Tawfik, D. S., & Griffiths, A. D.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- Weizmann Institute of Science (IL)
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Tawfik, Dan S
- Griffiths, Andrew D
- Venue
- Nature Methods
- Published
- 2006-07
- Volume
- 3
- Issue
- 7
- Pages
- 545-550
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W1968957319
- Cited by
- 358
- References
- 0
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 8 · 2023: 10 · 2022: 10 · 2021: 13 · 2020: 20 · 2019: 21 · 2018: 19 · 2017: 24 · 2016: 28 · 2015: 32 · 2014: 13 · 2013: 25 · 2012: 30
- FWCI
- 5.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Physical Sciences › Engineering › Biomedical Engineering › Innovative Microfluidic and Catalytic Techniques Innovation
- Keywords
- emulsion PCR, heteroduplex, PCR bias, single-molecule PCR, water-in-oil emulsions, DNA polymerase, haplotyping
- MeSH terms
- Emulsions, Surface-Active Agents, Water, Gene Library, Polymerase Chain Reaction, Nucleic Acid Amplification Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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