Template‐Directed Synthesis in 3′‐ and 5′‐Direction with Reversible Termination
Abstract
Extending both ways: A method for DNA-templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3′-extension; see scheme) and in the direction typical for conventional DNA synthesizers (5′-extension).
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Richert and co-workers recognized the potential of enzyme-free primer extension for genotyping and sequencing with reversible termination.
“In addition, Richert and co-workers also recognized the potential of enzyme-free primer extension for genotyping15 and sequencing with reversible termination.16,17”
From Sequencing of <scp>d</scp>/<scp>l</scp>-DNA and XNA by Templated-Synthesis (2025) · cites this paper as [16]
Cites
Selforganization of matter and the evolution of biological macromolecules
Eigen, M. (1971) · Die Naturwissenschaften · doi:10.1007/bf00623322 · Cited by 3,788 · see citing works
Efficient enzyme-free copying of all four nucleobases templated by immobilized RNA
Deck, C., Jauker, M., & Richert, C. (2011) · Nature Chemistry · doi:10.1038/nchem.1086 · Cited by 155 · see citing works
Self-citationRajamani, S., Ichida, J. K., Antal, T., Treco, D. A., Leu, K., Nowak, M. A., Szostak, J. W., & Chen, I. A. (2010) · Journal of the American Chemical Society · doi:10.1021/ja100780p · Cited by 118 · see citing works
Schrum, J. P., Ricardo, A., Krishnamurthy, M., Blain, J. C., & Szostak, J. W. (2009) · Journal of the American Chemical Society · doi:10.1021/ja906557v · Cited by 103 · see citing works
The prebiotic evolutionary advantage of transferring genetic information from RNA to DNA
Leu, K., Obermayer, B., Rajamani, S., Gerland, U., & Chen, I. A. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr525 · Cited by 85 · see citing works
Prebiotically plausible mechanisms increase compositional diversity of nucleic acid sequences
Derr, J., Manapat, M. L., Rajamani, S., Leu, K., Xulvi-Brunet, R., Joseph, I., Nowak, M. A., & Chen, I. A. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks065 · Cited by 74 · see citing works
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Fast and accurate nonenzymatic copying of an RNA-like synthetic genetic polymer
Zhang, S., Blain, J. C., Zielinska, D., Gryaznov, S. M., & Szostak, J. W. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1312329110 · Cited by 80 · see citing works
Copying of RNA Sequences without Pre‐Activation
Jauker, M., Griesser, H., & Richert, C. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201506592 · Cited by 72 · see citing works
Self-citationEnzyme-free genetic copying of DNA and RNA sequences
Sosson, M., & Richert, C. (2018) · Beilstein Journal of Organic Chemistry · doi:10.3762/bjoc.14.47 · Cited by 33 · see citing works
Self-citationSequencing of <scp>d</scp>/<scp>l</scp>-DNA and XNA by Templated-Synthesis
Joshi, S., Romanens, P., & Winssinger, N. (2025) · Journal of the American Chemical Society · doi:10.1021/jacs.5c00708 · Cited by 4 · see citing works
- Authors
- Kaiser, A., Spies, S., Lommel, T., & Richert, C.
- Affiliations
- University of Stuttgart (DE)
- ThyssenKrupp (Germany) (DE)
- Corresponding author
- Richert, Clemens
- ORCID
- Richert, Clemens
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2012-07-09
- Volume
- 51
- Issue
- 33
- Pages
- 8299-8303
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2171421260
- Cited by
- 31
- References
- 6
- Citations by year
- 2025: 2 · 2024: 3 · 2022: 1 · 2021: 1 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 6 · 2013: 4
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Angewandte Chemie International Edition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- DNA-templated synthesis, template synthesis, solid-phase synthesis, reversible termination, oligonucleotide synthesis
- MeSH terms
- Base Sequence, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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