The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Minimal Tags for Rapid Dual‐Color Live‐Cell Labeling and Super‐Resolution Microscopy
Abstract
The growing demands of advanced fluorescence and super‐resolution microscopy benefit from the development of small and highly photostable fluorescent probes. Techniques developed to expand the genetic code permit the residue‐specific encoding of unnatural amino acids (UAAs) armed with novel clickable chemical handles into proteins in living cells. Here we present the design of new UAAs bearing strained alkene side chains that have improved biocompatibility and stability for the attachment of tetrazine‐functionalized organic dyes by the inverse‐electron‐demand Diels–Alder cycloaddition (SPIEDAC). Furthermore, we fine‐tuned the SPIEDAC click reaction to obtain an orthogonal variant for rapid protein labeling which we termed selectivity enhanced (se) SPIEDAC. seSPIEDAC and SPIEDAC were combined for the rapid labeling of live mammalian cells with two different fluorescent probes. We demonstrate the strength of our method by visualizing insulin receptors (IRs) and virus‐like particles (VLPs) with dual‐color super‐resolution microscopy.
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Cites
Subdiffraction‐Resolution Fluorescence Imaging with Conventional Fluorescent Probes
Heilemann, M., van de Linde, S., Schüttpelz, M., Kasper, R., Seefeldt, B., Mukherjee, A., Tinnefeld, P., & Sauer, M. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200802376 · Cited by 1,866
Adding New Chemistries to the Genetic Code
Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
Bates, M., Huang, B., Dempsey, G. T., & Zhuang, X. (2007) · Science · doi:10.1126/science.1146598 · Cited by 1,504
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
Lukinavičius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., Mueller, V., Reymond, L., Corrêa Jr, I. R., Luo, Z.-G., et al. (2013) · Nature Chemistry · doi:10.1038/nchem.1546 · Cited by 893
Self-citationMeasuring image resolution in optical nanoscopy
Nieuwenhuizen, R. P. J., Lidke, K. A., Bates, M., Puig, D. L., Grünwald, D., Stallinga, S., & Rieger, B. (2013) · Nature Methods · doi:10.1038/nmeth.2448 · Cited by 800
Fluorescence nanoscopy by ground-state depletion and single-molecule return
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Influenza virus assembly and budding
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Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome
Neumann, H., Wang, K., Davis, L., Garcia-Alai, M., & Chin, J. W. (2010) · Nature · doi:10.1038/nature08817 · Cited by 622
Bioconjugation with Strained Alkenes and Alkynes
Debets, M. F., van Berkel, S. S., Dommerholt, J., Dirks, A. (. J., Rutjes, F. P. J. T., & van Delft, F. L. (2011) · Accounts of Chemical Research · doi:10.1021/ar200059z · Cited by 564
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Cited by
Chen, Z., Chen, M., Cheng, Y., Kowada, T., Xie, J., Zheng, X., & Rao, J. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.201913314 · Cited by 71
Metadata
- Authors
- Nikić, I., Plass, T., Schraidt, O., Szymański, J., Briggs, J. A. G., Schultz, C., & Lemke, E. A.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2014-01-28
- Volume
- 53
- Issue
- 8
- Pages
- 2245-2249
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2103346924
- Cited by
- 300
- References
- 29
- FWCI
- 15.75
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nikić, I., Plass, T., Schraidt, O., Szymański, J., Briggs, J. A. G., Schultz, C., & Lemke, E. A. (2014). Minimal Tags for Rapid Dual‐Color Live‐Cell Labeling and Super‐Resolution Microscopy. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201309847
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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