Claudin localization in cilia of the retinal pigment epithelium
Open accessAbstract
Using immunocytochemistry and confocal microscopy we demonstrate that claudin-immunoreactivity is a novel marker for retinal pigment epithelial cilia. Claudin-immunoreactivity obtained by polyclonal anti-claudin 1 antibody, which could crossreact with claudin 3, was colocalized with acetylated tubulin-immunoreactivity in cultured human retinal pigment epithelial cells. Claudin-immunoreactivity associated with the retinal pigment epithelium (RPE) cilia was more intense than was claudin-immunoreactivity in the junctional complex. Approximately two-thirds of the RPE cells in the rat contain cilia that are immunoreactive with acetylated tubulin on postnatal day 1, and a significant portion of these cilia label with the anti-claudin 1 antibody. Cilia decrease in frequency over subsequent postnatal days, and are absent by postnatal day 30. As RPE cilia decrease in number during postnatal rat development, claudin-immunoreactivity is lost earlier than acetylated tubulin, suggesting that the loss of claudin may initiate RPE cilium degeneration. Claudin-immunoreactivity was not evident in cilia of photoreceptor cells, epithelia of nasal mucosa, small intestine, or colon, suggesting that claudin may be a unique molecule in RPE cilia. These data suggest that cilia of the RPE, unlike cilia on other cell types, contain claudin, and that this molecule may play an important and specific role in the function and/or maintenance of RPE cilia.
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Rat RPE cells lose their cilia during the first postnatal weeks.
“It has been reported that rat RPE cells lose their cilia during the first postnatal weeks (Nishiyama et al., 2002).”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Nishiyama et al., 2002
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- Authors
- Nishiyama, K., Sakaguchi, H., Hu, J. G., Bok, D., & Hollyfield, J. G.
- Affiliations
- Cleveland Clinic (US)
- University of California, Los Angeles (US)
- Corresponding author
- Nishiyama, Kazutoshi
- Venue
- The Anatomical Record
- Publisher
- Wiley
- Published
- 2002-05-13
- Volume
- 267
- Issue
- 3
- Pages
- 196-203
- ISSN
- 0003-276X (ISSN-L)
- 1097-0185
- OpenAlex
- W2047902407
- Cited by
- 33
- References
- 27
- Citations by year
- 2025: 2 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2
- FWCI
- 0.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Anatomical Record (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Retina Research Foundation
- National Institutes of Health (5r01ey002362-23)
- National Eye Institute (EY02363, EY-00444, EY-00331, R01EY002363, EY 02362)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Keywords
- claudins, retinal pigment epithelium, cilia, claudin-1, claudin-3, acetylated tubulin, postnatal development, junctional complexes, photoreceptors
- MeSH terms
- Animals, Animals, Newborn, Cells, Cultured, Cilia, Eye, Humans, Immunohistochemistry, Membrane Proteins, Pigment Epithelium of Eye, Biomarkers, Rats, Sprague-Dawley, Microscopy, Confocal, Fluorescent Antibody Technique, Indirect, Tight Junctions, Rats, Claudin-1, Claudin-3
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