Metabolic flux analysis of the two astaxanthin‐producing microorganisms Haematococcus pluvialis and Phaffia rhodozyma in the pure and mixed cultures
Abstract
The two major astaxanthin‐producing microorganisms Phaffia rhodozyma and Haematococcus pluvialis exhibited elevated astaxanthin yields under the mixed culture regime, and the changes in flux distribution were investigated by means of metabolic flux analysis (MFA). In the mixed culture of the two strains, the carbon flux towards astaxanthin formation in P. rhodozyma increased by 20%, which may be due to the enriched oxygen evolved through the photosynthesis of H. pluvialis. On the other hand, the uptake of pyruvate and CO 2 excreted by P. rhodozyma also facilitated astaxanthin synthesis in H. pluvialis, which reduced 33% of the carbon flux exported from Calvin cycle to the catabolic pathway, and in turn raised the carbon flux to glyceraldehyde‐3‐phosphate by 25%. As a result, the carbon flux diverted to astaxanthin synthesis increased 2.8‐fold in comparison with that in the pure culture.
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Dong et al. (2006) mixed culture of P. rhodozyma and H. pluvialis revealed a 20% increase in carbon flux toward astaxanthin synthesis in P. rhodozyma, attributed to oxygen enrichment from H. pluvialis photosynthesis.
“The study revealed a 20% increase in carbon flux toward astaxanthin synthesis in P. rhodozyma, attributed to oxygen enrichment from H. pluvialis photosynthesis.”
From Harnessing Artificial Intelligence to Revolutionize Microalgae Biotechnology: Unlocking Sustainable Solutions for High-Value Compounds and Carbon Neutrality (2025) · cites this paper as [63]
In the mixed culture, H. pluvialis benefited from pyruvate and CO2 excreted by P. rhodozyma, reducing carbon flux from the Calvin cycle to catabolic pathways by 33% while increasing flux to glyceraldehyde-3-phosphate by 25%.
“Conversely, H. pluvialis benefited from pyruvate and CO2 excreted by P. rhodozyma, reducing carbon flux from the Calvin cycle to catabolic pathways by 33% while increasing flux to glyceraldehyde-3-phosphate by 25%.”
From Harnessing Artificial Intelligence to Revolutionize Microalgae Biotechnology: Unlocking Sustainable Solutions for High-Value Compounds and Carbon Neutrality (2025) · cites this paper as [63]
The interaction between P. rhodozyma and H. pluvialis resulted in a 2.8-fold increase in carbon flux directed toward astaxanthin synthesis compared to pure cultures.
“This interaction resulted in a 2.8-fold increase in carbon flux directed toward astaxanthin synthesis compared to pure cultures.”
From Harnessing Artificial Intelligence to Revolutionize Microalgae Biotechnology: Unlocking Sustainable Solutions for High-Value Compounds and Carbon Neutrality (2025) · cites this paper as [63]
Cites
SCHWENDER, J., SEEMANN, M., LICHTENTHALER, H. K., & ROHMER, M. (1996) · Biochemical Journal · doi:10.1042/bj3160073 · Cited by 370 · see citing works
Metabolic Flux Analysis in Synechocystis Using Isotope Distribution from 13C-Labeled Glucose
Yang, C., Hua, Q., & Shimizu, K. (2002) · Metabolic Engineering · doi:10.1006/mben.2002.0226 · Cited by 241 · see citing works
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Jagadevan, S., Banerjee, A., Banerjee, C., Guria, C., Tiwari, R., Baweja, M., & Shukla, P. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1181-1 · Cited by 275 · see citing works
Wu, Y., Shan, L., Zhao, W., & Lu, X. (2025) · Marine Drugs · doi:10.3390/md23050184 · Cited by 59 · see citing works
- Authors
- Dong, Q., Zhao, X., Ma, H., Xing, X., & Sun, N.
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2006-11
- Volume
- 1
- Issue
- 11
- Pages
- 1283-1292
- ISSN
- 1860-6768
- 1860-7314
- Cited by
- 18
- References
- 2
- Language
- en
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