The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
The BRET technology and its application to screening assays
Abstract
The bioluminescence resonance energy transfer (BRET) method is based on resonance energy transfer between a light‐emitting enzyme and a fluorescent acceptor. Since its first description in 1999, several versions of BRET have been described using different substrates and energy donor/acceptor couples. Today, BRET is considered as one of the most versatile techniques for studying the dynamics of protein‐protein interactions in living cells. Various studies have applied BRET‐based assays to screen new receptor ligands and inhibitors of disease‐related‐proteases. Inhibitors of protein‐protein interactions are likely to become a new major class of therapeutic drugs, and BRET technology is expected to play an important role in the identification of such compounds. This review describes the original BRET‐based methodology, more recent variants, and potential applications to drug screening.
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Small-molecule MDM2 antagonists reveal aberrant p53 signaling in cancer: implications for therapy
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“Several other variants of the YFP with identical excitatory properties are YFP topaz [21, 34, 36–38], YFP citrine [29, 32, 39], YFP venus [40, 41] and YPet [42, 43].”
BackgroundMethodInfluentialNew assay technologies for high-throughput screening
Silverman, L., Campbell, R., & Broach, J. R. (1998) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(98)80015-x · Cited by 151 · cited 1×
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MethodNutlin-3 inhibits the NFκB Pathway in a p53 Dependent Manner: Implications in Lung Cancer Therapy
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Cited by
Modulators of Protein–Protein Interactions
Milroy, L.-G., Grossmann, T. N., Hennig, S., Brunsveld, L., & Ottmann, C. (2014) · Chemical Reviews · doi:10.1021/cr400698c · Cited by 497
Chen, B., Yu, P., Chan, W. N., Xie, F., Zhang, Y., Liang, L., Leung, K. T., Lo, K. W., Yu, J., Tse, G. M. K., et al. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01679-y · Cited by 420
Keinan, N., Tyomkin, D., & Shoshan-Barmatz, V. (2010) · Molecular and Cellular Biology · doi:10.1128/mcb.00165-10 · Cited by 264
Self-luminescing BRET-FRET near-infrared dots for in vivo lymph-node mapping and tumour imaging
Xiong, L., Shuhendler, A. J., & Rao, J. (2012) · Nature Communications · doi:10.1038/ncomms2197 · Cited by 253
Dragulescu-Andrasi, A., Chan, C. T., De, A., Massoud, T. F., & Gambhir, S. S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100923108 · Cited by 186
Metadata
- Authors
- Bacart, J., Corbel, C., Jockers, R., Bach, S., & Couturier, C.
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2008-03
- Volume
- 3
- Issue
- 3
- Pages
- 311-324
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2147476348
- Cited by
- 178
- References
- 6
- FWCI
- 4.47
- Type
- journal article
- Language
- en
- Open access
- closed
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