The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
New assay technologies for high-throughput screening
Abstract
The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to interference from test compounds needed to function in a high-throughput screening mode. Similarly, novel cell-based assays are now being adapted for high-throughput screening, providing for in situ analysis of a variety of biological targets. Finally, recent advances in assay miniaturization mark a transition to ultra high-throughput screening, ensuring that identification of lead compounds will not be the rate-limiting step in finding new drugs.
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Metadata
- Authors
- Silverman, L., Campbell, R., & Broach, J. R.
- Affiliations
- Princeton University (US)
- Corresponding author
- Broach, James R
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 1998-06
- Volume
- 2
- Issue
- 3
- Pages
- 397-403
- ISSN
- 1367-5931 (ISSN-L)
- OpenAlex
- W2092399057
- Cited by
- 151
- References
- 55
- Citations by year
- 2025: 2 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 2 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 3
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Chemical Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Drug Discovery › Computational Drug Discovery Methods
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Keywords
- high-throughput screening, fluorescence correlation spectroscopy, cell-based assays, assay miniaturization, ultra-high-throughput screening, lead compound identification, early drug discovery
- MeSH terms
- Animals, Cell Line, Cell-Free System, Chemistry, Organic, Drug Evaluation, Preclinical, Energy Transfer, Mammals, Miniaturization, Spectrometry, Fluorescence, Transfection, Drug Design, Genes, Reporter
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