In Situ Bone Tissue Engineering With an Endogenous Stem Cell Mobilizer and Osteoinductive Nanofibrous Polymeric Scaffolds
Abstract
Classical bone tissue engineering involves the use of culture-expanded cells and scaffolds to produce tissue constructs for transplantation. Despite promising results, clinical adoption of these constructs has been limited due to various drawbacks, including extensive cell expansion steps, low cell survival rate upon transplantation, and the possibility of immuno-rejection. To bypass the ex vivo cell culture and transplantation process, the regenerative capacity of the host is exploited by mobilizing endogenous stem cells to the site of injury. Systemic injection of substance P (SP) induce mobilization of CD29 + CD105 + CD45 - cells from bone marrow and enhance bone tissue regeneration in a critical-sized calvarial bone defect model. To provide an appropriate environment for endogenous stem cells to survive and differentiate into osteogenic lineage cells, electrospun nanofibrous polycaprolactone (PCL) scaffolds are functionalized with hydroxyapatite (HA) particles via a polydopamine (PDA) coating to create highly osteoinductive PCL-PDA-HA scaffolds that are implanted in defects. The combination of the PCL-PDA-HA scaffold and SP treatment enhance in situ bone tissue formation in defects. Thus, this in situ bone regeneration strategy, which combines recruitment of endogenous stem cells from the bone marrow to defective sites and implantation of a highly biocompatible and osteoinductive cell-free scaffold system, has potential as an effective therapeutic in regenerative medicine…
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Compared to PCL, the PCL-PDA-HA scaffold demonstrated superior mechanical properties such as tensile strength and Young's modulus.
“Compared to PCL, the resulting scaffold demonstrated superior mechanical properties such as tensile strength and Young’s modulus.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [69]
An in situ bone regeneration strategy combining recruitment of endogenous stem cells from bone marrow to defective sites and implantation of a highly biocompatible and osteoinductive cell-free scaffold system has potential as an effective therapeutic in regenerative medicine.
“Thus, this in situ bone regeneration strategy, which combines the recruitment of endogenous stem cells from the bone marrow to defective sites, and implantation of a highly biocompatible and osteoinductive cell-free scaffold system, has potential as an effective therapeutic in regenerative medicine.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [69]
An effective strategy employs mobilization of stem cells to the injury site, supported by a bioactive implant material to promote bone generation.
“This work demonstrates that an effective strategy employs the mobilization of stem cells to the injury site, and is supported by a bioactive implant material to promote bone generation [69].”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [69]
The 3D microstructure of the PCL-PDA-HA scaffold was suitable for the survival and differentiation of stem cells.
“The 3D microstructure of the PCL-PDA-HA scaffold was suitable for the survival and differentiation of stem cells.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [69]
Cites
Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization
Ryu, J., Ku, S. H., Lee, H., & Park, C. B. (2010) · Advanced Functional Materials · doi:10.1002/adfm.200902347 · Cited by 778 · see citing works
Hong, H. S., Lee, J., Lee, E., Kwon, Y. S., Lee, E., Ahn, W., Jiang, M. H., Kim, J. C., & Son, Y. (2009) · Nature Medicine · doi:10.1038/nm.1909 · Cited by 349 · see citing works
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Kaushik, N., Nhat Nguyen, L., Kim, J. H., Choi, E. H., & Kumar Kaushik, N. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21186544 · Cited by 76 · see citing works
Shafiq, M., Wang, L., Zhi, D., Zhang, Q., Wang, K., Wang, L., Kim, D., Kong, D., & Kim, S. H. (2019) · Journal of Biomedical Materials Research Part B: Applied Biomaterials · doi:10.1002/jbm.b.34260 · Cited by 30 · see citing works
- Authors
- Lee, J. S., Jin, Y., Park, H., Yang, K., Lee, M. S., Yang, H. S., & Cho, S.
- Affiliations
- Yonsei University (KR)
- Dankook University (KR)
- Corresponding author
- Cho, Seung‐Woo
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2017-09-28
- Volume
- 12
- Issue
- 12
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2755906871
- Cited by
- 40
- References
- 2
- Citations by year
- 2026: 4 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 11 · 2020: 6 · 2019: 3 · 2018: 3 · 2017: 1
- FWCI
- 1.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Health and Welfare (HI13C1479)
- Ministry of Science, ICT and Future Planning (2016R1A5A1004694)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- substance P, bone marrow-derived stem cells, hydroxyapatite particles, polydopamine coating, osteoinduction, calvarial defect, cell-free scaffolds, osteogenic differentiation
- MeSH terms
- Animals, Bone and Bones, Bone Regeneration, Cells, Cultured, Osteogenesis, Polyesters, Skull, Stem Cells, Cell Culture Techniques, Tissue Engineering, Cell Proliferation, Mice, Tissue Scaffolds, Nanofibers
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