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Cited by 462

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

A microfabricated array bioreactor for perfused 3D liver culture

Powers, Mark J., Domansky, Karel, Kaazempur‐Mofrad, Mohammad R., Kalezi, Artemis, Capitano, Adam, Upadhyaya, Arpita, Kurzawski, Petra, Wack, Kathryn E., Stolz, Donna Beer, Kamm, Roger, Griffith, Linda G.·2002·Biotechnology and Bioengineering
DOI 10.1002/bit.10143
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Abstract

We describe the design, fabrication, and performance of a bioreactor that enables both morphogenesis of 3D tissue structures under continuous perfusion and repeated in situ observation by light microscopy. Three‐dimensional scaffolds were created by deep reactive ion etching of silicon wafers to create an array of channels (through‐holes) with cell‐adhesive walls. Scaffolds were combined with a cell‐retaining filter and support in a reactor housing designed to deliver a continuous perfusate across the top of the array and through the 3D tissue mass in each channel. Reactor dimensions were constructed so that perfusate flow rates meet estimated values of cellular oxygen demands while providing fluid shear stress at or below a physiological range (<2 dyne cm 2), as determined by comparison of numerical models of reactor fluid flow patterns to literature values of physiological shear stresses. We studied the behavior of primary rat hepatocytes seeded into the reactors and cultured for up to 2 weeks, and found that cells seeded into the channels rearranged extensively to form tissue like structures and remained viable throughout the culture period. We further observed that preaggregation of the cells into spheroidal structures prior to seeding improved the morphogenesis of tissue structure and maintenance of viability. We also demonstrate repeated in situ imaging of tissue structure and function using two‐photon microscopy. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 257–269, 2002.

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Metadata

Authors
Powers, M. J., Domansky, K., Kaazempur‐Mofrad, M. R., Kalezi, A., Capitano, A., Upadhyaya, A., Kurzawski, P., Wack, K. E., Stolz, D. B., Kamm, R., & Griffith, L. G.
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
2002-03-15
Volume
78
Issue
3
Pages
257-269
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
DOI
10.1002/bit.10143
OpenAlex
W2069703095
Cited by
462
References
45
FWCI
18.69
Type
journal article
Language
en
Open access
closed

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