Cited by 99Algal biology and biofuel productionFluid Dynamics and MixingAquatic Ecosystems and Phytoplankton Dynamics

Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique

Open access
Barbosa, Maria J., Zijffers, Jan Willem, Nisworo, Adrian, Vaes, Woulter, van Schoonhoven, Jan, Wijffels, René H.·2005·Biotechnology and Bioengineering

Abstract

Acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed at a constant acceleration rate, leading to different average light intensities inside the photobioreactor, can supply more information and reduce experimental time compared with chemostat cultivations. The A-stat was used to optimize the biomass and product yield of continuous cultures of the microalgae D. tertiolecta in a flat-panel reactor. In this study, four different accelerations were studied, a pseudo steady state was maintained at acceleration rates of 0.00016 and 0.00029 h(-2) and results were similar to those of the chemostat. An increase in the acceleration rate led to an increase in the deviation between results obtained in the A-stat and in the chemostats. We concluded that it is advantageous to use the A-stat instead of chemostats to determine culture characteristics and optimize a specific photobioreactor. The effect of average light intensity inside the photobioreactor on the production of vitamins C and E, lutein, and beta-carotene was studied using the A-stat. The highest concentrations of these products were 3.48 +/- 0.46, 0.33 +/- 0.06, 5.65 +/- 0.24, and 2.36 +/- 0.38 mg g(-1), respectively. These results were obtained at different average light intensities, showing the importance of optimizing each product on light intensity.

Integrity signals

Registry

Crossref · 10.1002/bit.20346

Cites

26 of 26 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Barbosa, M. J., Zijffers, J. W., Nisworo, A., Vaes, W., van Schoonhoven, J., & Wijffels, R. H.
Affiliations
Wageningen University & Research (NL)
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
2004-12-08
Volume
89
Issue
2
Pages
233-242
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
OpenAlex
W2113041579
Cited by
99
References
26
Citations by year
2026: 3 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 6 · 2021: 12 · 2020: 7 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 8 · 2014: 6 · 2013: 12 · 2012: 3
FWCI
1.04
Type
journal article
Language
en
Open access
Locations
  • Biotechnology and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • TNO Repository (submittedVersion)
  • Repository hosted by TU Delft Library (TU Delft) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
microalgae, Dunaliella tertiolecta, flat panel photobioreactor, light intensity optimization, biomass yield, vitamin C, vitamin E, lutein, beta-carotene, continuous culture, acceleration rate
MeSH terms
Chlorophyta, Ascorbic Acid, Carotenoids, Computer Simulation, Energy Transfer, Light, Models, Biological, Vitamin E, Gene Expression Regulation, Bacterial, Flow Injection Analysis, Cell Culture Techniques, Bioreactors, Cell Proliferation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review