Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique
Open accessAbstract
Acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed at a constant acceleration rate, leading to different average light intensities inside the photobioreactor, can supply more information and reduce experimental time compared with chemostat cultivations. The A-stat was used to optimize the biomass and product yield of continuous cultures of the microalgae D. tertiolecta in a flat-panel reactor. In this study, four different accelerations were studied, a pseudo steady state was maintained at acceleration rates of 0.00016 and 0.00029 h(-2) and results were similar to those of the chemostat. An increase in the acceleration rate led to an increase in the deviation between results obtained in the A-stat and in the chemostats. We concluded that it is advantageous to use the A-stat instead of chemostats to determine culture characteristics and optimize a specific photobioreactor. The effect of average light intensity inside the photobioreactor on the production of vitamins C and E, lutein, and beta-carotene was studied using the A-stat. The highest concentrations of these products were 3.48 +/- 0.46, 0.33 +/- 0.06, 5.65 +/- 0.24, and 2.36 +/- 0.38 mg g(-1), respectively. These results were obtained at different average light intensities, showing the importance of optimizing each product on light intensity.
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- Authors
- Barbosa, M. J., Zijffers, J. W., Nisworo, A., Vaes, W., van Schoonhoven, J., & Wijffels, R. H.
- Affiliations
- Wageningen University & Research (NL)
- ORCID
- Wijffels, René H.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2004-12-08
- Volume
- 89
- Issue
- 2
- Pages
- 233-242
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2113041579
- Cited by
- 99
- References
- 26
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 6 · 2021: 12 · 2020: 7 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 8 · 2014: 6 · 2013: 12 · 2012: 3
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- TNO Repository (submittedVersion)
- Repository hosted by TU Delft Library (TU Delft) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- microalgae, Dunaliella tertiolecta, flat panel photobioreactor, light intensity optimization, biomass yield, vitamin C, vitamin E, lutein, beta-carotene, continuous culture, acceleration rate
- MeSH terms
- Chlorophyta, Ascorbic Acid, Carotenoids, Computer Simulation, Energy Transfer, Light, Models, Biological, Vitamin E, Gene Expression Regulation, Bacterial, Flow Injection Analysis, Cell Culture Techniques, Bioreactors, Cell Proliferation
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