The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment
Abstract
Plant cell walls are composed primarily of cellulose, hemicelluloses, lignins, and pectins. Of these components, lignins exhibit unique chemistry and physiological functions. Although lignins can be used as a product feedstock or as a fuel, lignins are also generally seen as a barrier to efficient enzymatic breakdown of biomass to sugars. Indeed, many pretreatment strategies focus on removing a significant fraction of lignin from biomass to better enable saccharification. In order to better understand the fate of biomass lignins that remain with the solids following dilute acid pretreatment, we undertook a structural investigation to track lignins on and in biomass cell walls. SEM and TEM imaging revealed a range of droplet morphologies that appear on and within cell walls of pretreated biomass; as well as the specific ultrastructural regions that accumulate the droplets. These droplets were shown to contain lignin by FTIR, NMR, antibody labeling, and cytochemical staining. We provide evidence supporting the idea that thermochemical pretreatments reaching temperatures above the range for lignin phase transition cause lignins to coalesce into larger molten bodies that migrate within and out of the cell wall, and can redeposit on the surface of plant cell walls. This decompartmentalization and relocalization of lignins is likely to be at least as important as lignin removal in the quest to improve the digestibility of biomass for sugars and fuels production. © 2008 Wiley Periodicals, Inc.
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Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
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Pretreatment and Lignocellulosic Chemistry
Hu, F., & Ragauskas, A. (2012) · BioEnergy Research · doi:10.1007/s12155-012-9208-0 · Cited by 462
Tunable and functional deep eutectic solvents for lignocellulose valorization
Liu, Y., Deak, N., Wang, Z., Yu, H., Hameleers, L., Jurak, E., Deuss, P. J., & Barta, K. (2021) · Nature Communications · doi:10.1038/s41467-021-25117-1 · Cited by 452
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Metadata
- Authors
- Donohoe, B. S., Decker, S. R., Tucker, M. P., Himmel, M. E., & Vinzant, T. B.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2008-09-09
- Volume
- 101
- Issue
- 5
- Pages
- 913-925
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2146771344
- Cited by
- 678
- References
- 25
- FWCI
- 11.94
- Type
- journal article
- Language
- en
- Open access
- closed
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