The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Tailor‐made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus
Abstract
CRISPR/Cas‐derived RNA‐guided nucleases (RGNs) that can generate DNA double‐strand breaks (DSBs) at a specific sequence are widely used for targeted genome editing by induction of DSB repair in many organisms. The CRISPR/Cas system consists of two components: a single Cas9 nuclease and a single‐guide RNA (sgRNA). Therefore, the system for constructing RGNs is simple and efficient, but the utilization of RGNs in filamentous fungi has not been validated. In this study, we established the CRISPR/Cas system in the model filamentous fungus, Pyricularia oryzae, using Cas9 that was codon‐optimized for filamentous fungi, and the endogenous RNA polymerase (RNAP) III U6 promoter and a RNAP II fungal promoter for the expression of the sgRNA. We further demonstrated that RGNs could recognize the desired sequences and edit endogenous genes through homologous recombination‐mediated targeted gene replacement with high efficiency. Our system will open the way for the development of various CRISPR/Cas‐based applications in filamentous fungi. Biotechnol. Bioeng. 2015;112: 2543–2549. © 2015 Wiley Periodicals, Inc.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Genome engineering using the CRISPR-Cas9 system
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · cited 1×
“DOI 10.1002/bit.25662 2015 Wiley Periodicals, Inc. Biotechnology and Bioengineering, Vol. 112, No. 12, December, 2015 2543 et al., 2013; Qi et al., 2013), and the synthetic sgRNA guides the Cas9 nuclease to the specific genomic locus by forming an RNA/DNA hybrid at the target sequence.”
BackgroundInfluentialTALENs: A widely applicable technology for targeted genome editing
Joung, J. K., & Sander, J. D. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3486 · Cited by 1,650
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Sen, T., Barrow, C. J., & Deshmukh, S. K. (2019) · Frontiers in Nutrition · doi:10.3389/fnut.2019.00007 · Cited by 367
Nuclear autonomy in multinucleate fungi
Roberts, S. E., & Gladfelter, A. S. (2015) · Current Opinion in Microbiology · doi:10.1016/j.mib.2015.08.009 · Cited by 45
Shen, Q., Ruan, H., Zhang, H., Wu, T., Zhu, K., Han, W., Dong, R., Ming, T., Qi, H., & Zhang, Y. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1375120 · Cited by 38
Metadata
- Authors
- Arazoe, T., Miyoshi, K., Yamato, T., Ogawa, T., Ohsato, S., Arie, T., & Kuwata, S.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2015-07-14
- Volume
- 112
- Issue
- 12
- Pages
- 2543-2549
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2162762486
- Cited by
- 207
- References
- 5
- FWCI
- 8.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- Arazoe, T., Miyoshi, K., Yamato, T., Ogawa, T., Ohsato, S., Arie, T., & Kuwata, S. (2015). Tailor‐made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.25662
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