Evolution in Reverse: Engineering a D‐Xylose‐Specific Xylose Reductase
Abstract
Staying faithful: Xylose reductases (XRs) are highly promiscuous enzymes and are of great interest for the biosynthesis of xylitol—a very desirable sugar substitute. However, the expensive purification of the substrate D-xylose from the impurity L-arabinose plagues the industrial implementation of XRs. We have engineered a XR that preferentially reduces D-xylose over L-arabinose (see figure). This is one of the very few cases in which a promiscuous enzyme has been engineered to prefer one natural substrate over another.
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Sugar fermentations to produce polyols such as sorbitol, mannitol, xylitol are feasible although they cannot yet compete with chemical reduction economically.
“Sugar fermentations to produce these polyols are also feasible although it cannot yet compete with the chemical reduction economically.[149][150][151]”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [150]
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- Authors
- Nair, N. U., & Zhao, H.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Zhao, Huimin
- Venue
- ChemBioChem
- Publisher
- Wiley
- Published
- 2008-05-15
- Volume
- 9
- Issue
- 8
- Pages
- 1213-1215
- ISSN
- 1439-4227 (ISSN-L)
- 1439-7633
- OpenAlex
- W2156654543
- Cited by
- 52
- References
- 18
- Citations by year
- 2026: 3 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ChemBioChem (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- xylose reductase, D-xylose, L-arabinose, substrate specificity, enzyme engineering, sugar alcohols, substrate promiscuity, reverse engineering
- MeSH terms
- Aldehyde Reductase, Kinetics, Mutation, Neurospora crassa, Substrate Specificity, Xylose, Protein Engineering, Molecular Structure, Evolution, Molecular
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