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Cited by 81RNA and protein synthesis mechanismsFungal and yeast genetics researchMicrotubule and mitosis dynamics

The DOI matches its Crossref record.

Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation

Valouev, Igor A., Kushnirov, Vitaly V., Ter‐Avanesyan, Michael D.·2002·Cell Motility
DOI 10.1002/cm.10040
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Abstract

Termination of translation in eukaryotes is controlled by two interacting polypeptide chain release factors, eRF1 and eRF3. eRF1 recognizes nonsense codons UAA, UAG, and UGA, while eRF3 stimulates polypeptide release from the ribosome in a GTP- and eRF1-dependent manner. In the yeast Saccharomyces cerevisiae, eRF1 and eRF3 are encoded by the SUP45 and SUP35 genes, respectively. Here we show that in yeast shortage of any one of the release factors was accompanied by a reduction in the levels of the other release factor and resulted in a substantial increase of nonsense codon readthrough. Besides, repression of the genes encoding these factors caused different effects on cell morphology. Repression of the SUP35 gene caused accumulation of cells of increased size with large buds. This was accompanied by the disappearance of actin cytoskeletal structures, impairment of the mitotic spindle structure, and defects in nuclei division and segregation in mitosis. The evolutionary conserved C-terminal domain of eRF3 similar to the elongation factor EF-1alpha was responsible for these effects. Repression of the SUP45 gene caused accumulation of unbudded cells with 2C and higher DNA content, indicating that DNA replication is uncoupled from budding. The data obtained suggest that eRF1 and eRF3 play additional, nontranslational roles in the yeast cell.

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Research works printed but not linked: 69

  1. 10.1016/0076-6879(91)94054-G · DOI
  2. 10.1093/genetics/153.1.81 · DOI
  3. 10.1002/(SICI)1097-0169(1998)39:4<286::AID-CM4>3.0.CO;2-1 · DOI
  4. 10.1021/bi971292s · DOI
  5. 10.1021/bi9712910 · DOI
  6. 10.1002/yea.320070609 · DOI
  7. 10.1007/s002940050529 · DOI
  8. Botstein D, 1997, The molecular and cellular biology of the yeast Saccharomyces, 1
  9. 10.1016/0003-2697(76)90527-3 · DOI · Paper page
  10. 10.1146/annurev.mi.29.100175.001203 · DOI
  11. 10.1007/978-1-4684-5377-5_9 · DOI
  12. 10.1016/S0968-0004(00)88998-7 · DOI
  13. 10.1017/S1355838200992392 · DOI
  14. 10.1101/gad.12.11.1665 · DOI
  15. 10.1093/genetics/128.3.513 · DOI
  16. 10.1002/(SICI)1097-0061(199710)13:13<1265::AID-YEA175>3.0.CO;2-# · DOI
  17. 10.1105/tpc.6.6.893 · DOI
  18. 10.1038/372701a0 · DOI
  19. Frolova L, 1996, Eukaryotic polypeptide chain release factor eRF3 is an eRF1‐ and ribosome‐dependent guanosine triphosphatase, RNA, 2, 334
  20. 10.1046/j.1432-1327.1998.2560036.x · DOI
  21. 10.1038/35085604 · DOI
  22. Gagny B, 1998, Identification of the genes encoding the cytosolic translation release factors from Podospora anserina and analysis of their role during the life cycle, Genetics, 149, 1763, 10.1093/genetics/149.4.1763 · DOI
  23. 10.1093/emboj/20.8.1993 · DOI
  24. 10.1016/0378-1119(88)90185-0 · DOI
  25. 10.1038/415180a · DOI
  26. Hanahan D, 1985, DNA cloning: a practical approach, 109
  27. 10.1016/S0092-8674(00)81080-6 · DOI
  28. Inge‐Vechtomov SG, 1970, Recessive super‐suppressors in yeast, Genetika, 6, 103
  29. 10.1002/cm.970190403 · DOI
  30. 10.1017/S1355838298971874 · DOI
  31. 10.1016/S0167-7012(00)00136-6 · DOI
  32. 10.1093/oxfordjournals.jbchem.a021309 · DOI
  33. 10.1016/S0092-8674(00)81216-7 · DOI
  34. 10.1016/0378-1119(88)90223-5 · DOI
  35. 10.1038/227680a0 · DOI · Paper page
  36. 10.1128/MCB.17.6.3164 · DOI
  37. 10.1083/jcb.135.4.953 · DOI
  38. 10.1038/47048 · DOI · Paper page
  39. 10.1002/yea.320070608 · DOI
  40. Munshi R, 2001, Overexpression of translation elongation factor 1α affects the organization and function of the actin cytoskeleton in yeast, Genetics, 157, 1425, 10.1093/genetics/157.4.1425 · DOI
  41. 10.1126/science.273.5275.622 · DOI
  42. 10.1002/j.1460-2075.1996.tb00675.x · DOI
  43. 10.1128/MCB.17.5.2798 · DOI
  44. 10.1016/0076-6879(91)94055-H · DOI
  45. 10.1016/0076-6879(91)94043-C · DOI
  46. Pruyene D, 2000, Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states, J Cell Sci, 113, 365, 10.1242/jcs.113.3.365 · DOI
  47. Pruyene D, 2000, Polarization of cell growth in yeast. II. The role of cortical actin cytoskeleton, J Cell Sci, 113, 571, 10.1242/jcs.113.4.571 · DOI
  48. 10.1016/0378-1119(87)90232-0 · DOI
  49. Rose MD, 1990, Methods in yeast genetics
  50. Sambrook J, 1989, Molecular cloning: a laboratory manual
  51. Sherman F, 1986, Methods in yeast genetics
  52. 10.1126/science.7939665 · DOI
  53. 10.1016/S0092-8674(00)80667-4 · DOI
  54. Speenivasan A, 1999, The Elm1 kinase functions in a mitotic signaling network in budding yeast, Mol Cell Biol, 19, 7983, 10.1128/MCB.19.12.7983 · DOI
  55. 10.1002/j.1460-2075.1995.tb00111.x · DOI
  56. 10.1111/j.1365-2958.1996.tb02634.x · DOI
  57. 10.1073/pnas.051013498 · DOI
  58. 10.1046/j.1365-2443.1999.00279.x · DOI
  59. Surguchov AP, 1984, Ribosomal suppression in eukaryotes, Physicochem Biol Rev, 4, 147
  60. Ter‐Avanesyan MD, 1994, The SUP35 omnipotent suppressor is involved in the maintenance of the non‐Mendelian determinant [PSI +] in the yeast Saccharomyces cerevisiae, Genetics, 137, 671, 10.1093/genetics/137.3.671 · DOI
  61. 10.1111/j.1365-2958.1993.tb01159.x · DOI
  62. 10.1007/s002940050098 · DOI
  63. 10.1073/pnas.76.9.4350 · DOI
  64. 10.1038/sj.onc.1201724 · DOI
  65. 10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W · DOI
  66. Vanoni M, 1983, Structural heterogeneity in populations of the budding yeast S. cerevisiae, J Bacteriol, 156, 1282, 10.1128/jb.156.3.1282-1291.1983 · DOI
  67. 10.1128/MCB.16.10.5491 · DOI
  68. 10.1038/347494a0 · DOI
  69. 10.1002/j.1460-2075.1995.tb00078.x · DOI

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Metadata

Authors
Valouev, I. A., Kushnirov, V. V., & Ter‐Avanesyan, M. D.
Affiliations
Institute of Experimental Cardiology (RU)
Corresponding author
Ter‐Avanesyan, Michael D.
ORCID
Venue
Cell Motility
Publisher
Wiley
Published
2002-06-19
Volume
52
Issue
3
Pages
161-173
ISSN
0886-1544 (ISSN-L)
DOI
10.1002/cm.10040
OpenAlex
W2015153105
Cited by
81
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 5 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 5 · 2013: 2 · 2012: 3
FWCI
2.09
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Cell Motility and the Cytoskeleton (publishedVersion)
  • PubMed (publishedVersion)
Funders
Howard Hughes Medical Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
eRF1, eRF3, translation termination, cytoskeletal organization, actin cytoskeleton, mitotic spindle, cell cycle regulation, nuclear division, DNA replication, DNA content, cell morphology, Saccharomyces cerevisiae, Sup35, SUP45
MeSH terms
Actins, Cell Cycle, Cytoskeleton, Spindle Apparatus, Peptide Termination Factors, Saccharomyces cerevisiae, Protein Biosynthesis, Codon, Nonsense

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CoChat. (2026). Citation check: Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation. https://app.cochat.ai/papers/10.1002/cm.10040
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Valouev, I. A., Kushnirov, V. V., & Ter‐Avanesyan, M. D. (2002). Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation. Cell Motility. https://doi.org/10.1002/cm.10040