The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Guiding cell migration in 3D: A collagen matrix with graded directional stiffness
Abstract
While matrix stiffness has been implicated in cell adhesion and migration, most studies have focused on the effects of substrate stiffness in 2D. The present work describes a novel continuous stiffness gradient model for studying such processes in 3D. Wedge‐shaped collagen scaffolds were compressed to produce sheets of a desired (0.1 mm) uniform thickness, but with increasing collagen density along the length of the sheet. Dynamic mechanical analysis, carried out on 1 mm wide strips obtained from the two ends and the middle of each sheet, showed that the elastic modulus increased from 1057 ± 487 kPa to 2305 ± 693 kPa at the soft and stiff end respectively and was 1835 ± 31 kPa in the middle. In constructs seeded with agarose marker beads prior to compression, mean agarose bead density rose from 10 ± 1 to 71 ± 12 at the soft and stiff end respectively and was 19 ± 5 in the middle, indicating successful engineering of a density gradient corresponding to the measured stiffness gradient. Growth‐arrested human dermal fibroblasts, initially seeded evenly within such constructs, accumulated preferentially towards the stiff part of the gradient after 3 and 6 days in culture. Durotactic migration was significant after 6 days. This model provides a new means for studying cellular mechanotaxis and patterning cells which is controllable, biomimetic and in 3D. Cell Motil. Cytoskeleton 66: 121–128, 2009. © 2009 Wiley‐Liss, Inc.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Formation and function of the myofibroblast during tissue repair
Hinz, B. (2007) · Journal of Investigative Dermatology · doi:10.1038/sj.jid.5700613 · Cited by 1,658
Cell adaptation to a physiologically relevant ECM mimic with different viscoelastic properties
Ghosh, K., Pan, Z., Guan, E., Ge, S., Liu, Y., Nakamura, T., Ren, X.-D., Rafailovich, M., & Clark, R. A. F. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2006.09.038 · Cited by 386
Cited by
Cox, T. R., & Erler, J. T. (2011) · Disease Models & Mechanisms · doi:10.1242/dmm.004077 · Cited by 1,569
Stiffening hydrogels to probe short- and long-term cellular responses to dynamic mechanics
Guvendiren, M., & Burdick, J. A. (2012) · Nature Communications · doi:10.1038/ncomms1792 · Cited by 707
Provenzano, P. P., & Keely, P. J. (2011) · Journal of Cell Science · doi:10.1242/jcs.067009 · Cited by 494
The mechanical memory of lung myofibroblasts
Balestrini, J. L., Chaudhry, S., Sarrazy, V., Koehler, A., & Hinz, B. (2012) · Integrative Biology · doi:10.1039/c2ib00149g · Cited by 386
Physical Aspects of Cell Culture Substrates: Topography, Roughness, and Elasticity
Ross, A. M., Jiang, Z., Bastmeyer, M., & Lahann, J. (2012) · Small · doi:10.1002/smll.201100934 · Cited by 357
Collagen—emerging collagen based therapies hit the patient
Abou Neel, E. A., Bozec, L., Knowles, J. C., Syed, O., Mudera, V., Day, R., & Hyun, J. K. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.08.010 · Cited by 296
Self-citationRaab, M., Swift, J., P. Dingal, P. C. D., Shah, P., Shin, J.-W., & Discher, D. E. (2012) · Journal of Cell Biology · doi:10.1083/jcb.201205056 · Cited by 285
Extracellular Matrix Cues Regulate Mechanosensing and Mechanotransduction of Cancer Cells
Mierke, C. T. (2024) · Cells · doi:10.3390/cells13010096 · Cited by 146
The Fluid Mechanics of Cancer and Its Therapy
Koumoutsakos, P., Pivkin, I., & Milde, F. (2013) · Annual Review of Fluid Mechanics · doi:10.1146/annurev-fluid-120710-101102 · Cited by 129
Metadata
- Authors
- Hadjipanayi, E., Mudera, V., & Brown, R. A.
- Venue
- Cell Motility
- Publisher
- Wiley
- Published
- 2009-01-23
- Volume
- 66
- Issue
- 3
- Pages
- 121-128
- ISSN
- 0886-1544 (ISSN-L)
- OpenAlex
- W2106324055
- Cited by
- 246
- References
- 5
- FWCI
- 6.88
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
