Phase 1/2 study of immunotherapy with dendritic cells pulsed with autologous tumor lysate in patients with refractory bone and soft tissue sarcoma
Open accessAbstract
BACKGROUND: There are limited options for the curative treatment of refractory bone and soft tissue sarcomas. The purpose of this phase 1/2 study was to assess the immunological and clinical effects of dendritic cells (DCs) pulsed with autologous tumor lysate (TL) in patients with advanced bone and soft tissue sarcomas. METHODS: Thirty‐seven patients with metastatic or recurrent sarcomas were enrolled in this study. Peripheral blood mononuclear cells obtained from the patients were suspended in media containing interleukin 4 (IL‐4) and granulocyte‐macrophage colony‐stimulating factor. Subsequently, these cells were treated with TL, tumor necrosis factor α, and OK‐432. The DCs were injected into the inguinal or axillary region. One treatment course comprised 6 weekly DC injections. The toxicity, clinical response (tumor volume, serum interferon‐γ [IFN‐γ], and serum IL‐12), and oncological outcomes were observed. RESULTS: In total, 47 courses of DC therapy were performed in 37 patients. No severe adverse events or deaths associated with the DC injections were observed in the study patients. Increased serum IFN‐γ and IL‐12 levels were observed 1 month after the DC injection. Among the 37 patients, 35 patients were assessed for clinical responses: 28 patients showed tumor progression, 6 patients had stable disease, and 1 patient showed a partial response 8 weeks after the DC injection. The 3‐year overall and progression‐free survival rates of the patients were 42.3% and 2.9%, respectively. CONCLUSIONS: Although DC therapy appears safe and resulted in an immunological response in patients with refractory sarcoma, it resulted in an improvement of the clinical outcome in only a small number of patients. Cancer 2017;123:1576–1584. © 2017 American Cancer Society.
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In clinical trials, DC vaccines showed limited effect for the treatment of osteosarcoma.
“However, in clinical trials, DC vaccines showed limited effect for the treatment of osteosarcoma [65, 66].”
From Advances on immunotherapy for osteosarcoma (2024) · cites this paper as [66]
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- Authors
- Miwa, S., Nishida, H., Tanzawa, Y., Takeuchi, A., Hayashi, K., Yamamoto, N., Mizukoshi, E., Nakamoto, Y., Kaneko, S., & Tsuchiya, H.
- Affiliations
- Kanazawa University (JP)
- University of Fukui (JP)
- Corresponding author
- Miwa, Shinji
- ORCID
- Venue
- Cancer
- Publisher
- Wiley
- Published
- 2017-02-27
- Volume
- 123
- Issue
- 9
- Pages
- 1576-1584
- ISSN
- 0008-543X (ISSN-L)
- 1097-0142
- OpenAlex
- W2592814855
- Cited by
- 95
- References
- 39
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 9 · 2023: 13 · 2022: 11 · 2021: 11 · 2020: 13 · 2019: 8 · 2018: 10 · 2017: 4
- FWCI
- 4.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cancer (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Grant-in-Aid for Scientific Research (B) (25293318)
- Grant-in-Aid for Young Japanese Scientists (B) (16K20042)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Keywords
- dendritic cell immunotherapy, autologous tumor lysate, bone sarcoma, soft tissue sarcoma, refractory sarcoma, OK-432, IL-4, GM-CSF, TNF-α, interferon-gamma, IL-12, treatment safety, clinical response, overall survival, progression-free survival
- MeSH terms
- Adolescent, Adult, Aged, Antineoplastic Agents, Bone Neoplasms, Child, Chondrosarcoma, Dendritic Cells, Female, Humans, Immunotherapy, Interferon-gamma, Leiomyosarcoma, Leukocytes, Mononuclear, Male, Middle Aged, Picibanil, Sarcoma, Osteosarcoma, Soft Tissue Neoplasms, Sarcoma, Synovial, Tumor Necrosis Factor-alpha, Interleukin-4, Granulocyte-Macrophage Colony-Stimulating Factor, Treatment Outcome, Sarcoma, Clear Cell, Disease-Free Survival, Interleukin-12, Histiocytoma, Malignant Fibrous, Young Adult
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