Synaptic distribution of the endocytic accessory proteins AP180 and CALM
Open accessAbstract
Clathrin-coated vesicles mediate a variety of endocytosis pathways in cells, including endocytic events at synapses. AP180 and clathrin assembly lymphoid myeloid leukemia protein (CALM) are clathrin accessory proteins that promote the formation of clathrin-coated vesicles. Both proteins bind to membrane lipids through their epsin N-terminal homology domains and interact with clathrin and related protein components through their carboxyl-terminal peptide motifs. We examine their neuronal expression and synaptic distribution. We show that both proteins are detected in synapses but demonstrate different distribution patterns. AP180 is located predominantly in presynaptic profiles, whereas CALM is found nonselectively in pre- and postsynaptic profiles and also in perisynaptic processes. These observations reveal an unexpected relationship between AP180 and the presumed non-neuronal homologue CALM. We propose that both AP180 and CALM function as endocytic accessory proteins at synapses, but each may regulate distinct clathrin pathways.
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PICALM appears to be involved in directing the trafficking of VAMP2, a SNARE protein involved in synaptic vesicle fusion.
“Of relevance to AD, PICALM appears to be involved in directing the trafficking of VAMP2.VAMP2 is a SNARE protein that plays a prominent role in the fusion of synaptic vesicles to the presynaptic membrane in neurotransmitter release, a process that is crucial to neuronal function39.”
From Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer's disease (2009) · cites this paper as [39]
The Discussion reports CALM at synapses and suggests it may help regulate synaptic clathrin pathways. The introductory section links mislocalization of synaptobrevin to a mutation in an AP180 homolog, not to CALM. Our copy does not establish that CALM/PICALM directs VAMP2 trafficking or describe VAMP2’s role in vesicle fusion. (LLM, conf=0.77)
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Kirchhausen, T. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.699 · Cited by 560 · see citing works
Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane.
Ahle, S., Mann, A., Eichelsbacher, U., & Ungewickell, E. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02897.x · Cited by 354 · see citing works
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Research works printed but not linked: 3
- De Camili P, Slepnev V, Shupliakov O, Brodin L. 2000. Synaptic vesicle endocytosis. In: Sudhof TC, Stevens CF, editors. Synapses. Baltimore, MD: The Johns Hopkins University Press. p 217-274.
- Palay SL, Chan-Palay V. 1974. Cerebellar cortex: cytology and organiza- tion. Berlin: Springer.
- Petralia RS, Wenthold RJ. 1999. Immunocytochemistry of NMDA recep- tors. In: Li M, editor. Methods in molecular biology: NMDA receptor protocols. Totowa, NJ: Humana Press.
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Harold, D., Abraham, R., Hollingworth, P., Sims, R., Gerrish, A., Hamshere, M. L., Pahwa, J. S., Moskvina, V., Dowzell, K., Williams, A., et al. (2009) · Nature Genetics · doi:10.1038/ng.440 · Cited by 3,326 · see citing works
The Genetics of Alzheimer Disease: Back to the Future
Bertram, L., Lill, C. M., & Tanzi, R. E. (2010) · Neuron · doi:10.1016/j.neuron.2010.10.013 · Cited by 834 · see citing works
Endo-lysosomal dysregulations and late-onset Alzheimer’s disease: impact of genetic risk factors
Van Acker, Z. P., Bretou, M., & Annaert, W. (2019) · Molecular Neurodegeneration · doi:10.1186/s13024-019-0323-7 · Cited by 222 · see citing works
- Authors
- Yao, P. J., Petralia, R. S., Bushlin, I., Wang, Y., & Furukawa, K.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Yao, Pamela J.
- Venue
- The Journal of Comparative Neurology
- Publisher
- Wiley
- Published
- 2004
- Volume
- 481
- Issue
- 1
- Pages
- 58-69
- ISSN
- 0021-9967 (ISSN-L)
- 1096-9861
- OpenAlex
- W2120434320
- Cited by
- 49
- References
- 2
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 2 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- public-domain
- doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 4261 d)
- Locations
- The Journal of Comparative Neurology (publishedVersion, public-domain)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- AP180, CALM, clathrin-coated vesicles, synaptic endocytosis, presynaptic terminals, postsynaptic terminals, neuronal expression, synaptic distribution
- MeSH terms
- Animals, Cells, Cultured, Endocytosis, Hippocampus, Neurons, Synapses, Tissue Distribution, PC12 Cells, Presynaptic Terminals, Reverse Transcriptase Polymerase Chain Reaction, Clathrin-Coated Vesicles, Monomeric Clathrin Assembly Proteins, Rats
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