Potential and limitations of X‐Ray micro‐computed tomography in arthropod neuroanatomy: A methodological and comparative survey
Open accessAbstract
Classical histology or immunohistochemistry combined with fluorescence or confocal laser scanning microscopy are common techniques in arthropod neuroanatomy, and these methods often require time‐consuming and difficult dissections and sample preparations. Moreover, these methods are prone to artifacts due to compression and distortion of tissues, which often result in information loss and especially affect the spatial relationships of the examined parts of the nervous system in their natural anatomical context. Noninvasive approaches such as X‐ray micro‐computed tomography (micro‐CT) can overcome such limitations and have been shown to be a valuable tool for understanding and visualizing internal anatomy and structural complexity. Nevertheless, knowledge about the potential of this method for analyzing the anatomy and organization of nervous systems, especially of taxa with smaller body size (e.g., many arthropods), is limited. This study set out to analyze the brains of selected arthropods with micro‐CT, and to compare these results with available histological and immunohistochemical data. Specifically, we explored the influence of different sample preparation procedures. Our study shows that micro‐CT is highly suitable for analyzing arthropod neuroarchitecture in situ and allows specific neuropils to be distinguished within the brain to extract quantitative data such as neuropil volumes. Moreover, data acquisition is considerably faster compared with many classical histological techniques. Thus, we conclude that micro‐CT is highly suitable for targeting neuroanatomy, as it reduces the risk of artifacts and is faster than classical techniques. J. Comp. Neurol. 523:1281–1295, 2015. © 2015 Wiley Periodicals, Inc.
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To image insects with conventional micro-CT, staining and/or drying is often required to discriminate between tissues.
“to image insects with conventional micro-CT, staining and/or drying is often required to discriminate between tissues 16 – 19”
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- Authors
- Sombke, A., Lipke, E., Michalik, P., Uhl, G., & Harzsch, S.
- Affiliations
- Universität Greifswald (DE)
- Corresponding author
- Sombke, Andy
- Venue
- Journal of Comparative Neurology
- Publisher
- Wiley
- Published
- 2015-03-02
- Volume
- 523
- Issue
- 8
- Pages
- 1281-1295
- ISSN
- 0021-9967 (ISSN-L)
- 1096-9861
- PMC
- PMC4409823
- OpenAlex
- W2097179532
- Cited by
- 144
- References
- 63
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 15 · 2023: 10 · 2022: 10 · 2021: 15 · 2020: 14 · 2019: 19 · 2018: 20 · 2017: 10 · 2016: 14 · 2015: 4
- FWCI
- 7.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The Journal of Comparative Neurology (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (HA 2540/8, DFG INST 292/119-1 FUGG, DFG INST 292/120-1 FUGG)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
- Health Sciences › Medicine › Urology › Comparative Animal Anatomy Studies
- Keywords
- micro-computed tomography, sample preparation artifacts, non-invasive brain imaging, comparative neuroanatomy, insect nervous system
- MeSH terms
- Animals, Arthropods, Brain, Image Processing, Computer-Assisted, Organ Size, Species Specificity, Imaging, Three-Dimensional, X-Ray Microtomography
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