In situ hybridization analysis of vasopressin gene transcription in the paraventricular and supraoptic nuclei of the rat: Regulation by stress and glucocorticoids
Abstract
Hypothalamic arginine vasopressin-containing neurons are prime elements in central circuits regulating the hypothalamo-pituitary-adrenocortical stress response. It is known that release and synthesis of vasopressin are cued by stressful stimuli. The present study was designed to assess effects of stress on vasopressin transcription and mRNA expression in defined populations of vasopressin neurons in the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Intron- and exon-directed in situ hybridization analyses were used to examine stress regulation of vasopressin heteronuclear (hn) gene transcription and mRNA levels. Actions of glucocorticoids on vasopressin induction were tested using adrenalectomized rats implanted with subcutaneous pellets delivering a constant, physiological dose of corticosterone. Pellet implantation into adrenalectomized rats allows for normal pituitary-adrenal tone in the absence of the ability to mount glucocorticoid stress responses. Elevation of vasopressin heteronuclear (hn) RNA in the medial parvicellular PVN was observed in both normal and adrenalectomized-corticosterone replaced rats as early as 30 minutes after stress initiation. In control rats, vasopressin hnRNA levels returned to baseline by 120 minutes. In contrast, vasopressin hnRNA remained elevated 120 minutes post-restraint in adrenalectomized-corticosterone replaced rats, indicating that the glucocorticoid stress response acts to rapidly inhibit vasopressin transcription. S…
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In adrenalectomized rats implanted with pellets delivering physiological CORT levels, Avp transcription and mRNA levels are significantly elevated.
“In a study using adrenalectomized rats implanted with pellets delivering physiological CORT levels, Avp transcription and mRNA levels were significantly elevated (Herman, 1995).”
From Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017) · cites this paper as Herman, 1995
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Pattern and time course of immediate early gene expression in rat brain following acute stress
Cullinan, W. E., Herman, J. P., Battaglia, D. F., Akil, H., & Watson, S. J. (1995) · Neuroscience · doi:10.1016/0306-4522(94)00355-9 · Cited by 1,056 · see citing works
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Stressor specificity of central neuroendocrine responses: implications for stress-related disorders
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“In a study using adrenalectomized rats implanted with pellets delivering physiological CORT levels, Avp transcription and mRNA levels were significantly elevated (Herman, 1995).”
BackgroundDumais, K. M., & Veenema, A. H. (2016) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2015.04.003 · Cited by 520 · see citing works
“As such, VP is part of the hypothalamic–pituitary–adrenal (HPA) axis, a system involved in the neuroendocrine stress response (Herman, 1995; Volpi et al., 2004).”
BackgroundMeasurement of Immediate‐Early Gene Activation‐ <i>c‐fos</i> and Beyond
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“…CRFexpressing neurons commonly triggers neuropeptide gene expression to replenish depleted stores and can result in stimulusspecific alterations in cellular phenotype, including induced AVP expression (Lightman and Young, 1988; Herman et al., 1992; Herman, 1995; Makino et al., 1995).”
BackgroundResultInfluentialHypothalamic-pituitary – adrenal axis-feedback control
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Chronic variable stress alters hypothalamic–pituitary–adrenal axis function in the female mouse
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- Authors
- Herman, J. P.
- Affiliations
- University of Kentucky (US)
- Corresponding author
- Herman, James P.
- ORCID
- Herman, James P.
- Venue
- Journal of Comparative Neurology
- Publisher
- Wiley
- Published
- 1995-12-04
- Volume
- 363
- Issue
- 1
- Pages
- 15-27
- ISSN
- 0021-9967 (ISSN-L)
- 1096-9861
- OpenAlex
- W1995397241
- Cited by
- 129
- References
- 2
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 5 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Comparative Neurology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Kentucky
- National Institute of Mental Health (MH49698)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- vasopressin, paraventricular nucleus, supraoptic nucleus, glucocorticoids, corticosterone, adrenalectomy, restraint stress, parvicellular neurons, magnocellular neurons, hypothalamic-pituitary-adrenal axis, negative feedback
- MeSH terms
- Animals, Glucocorticoids, Male, Paraventricular Hypothalamic Nucleus, RNA, Messenger, Supraoptic Nucleus, Transcription, Genetic, Vasopressins, Gene Expression, Rats, Sprague-Dawley, In Situ Hybridization, Rats
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