The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysis
Open accessAbstract
A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solved in the new modification by trypsinization of the unfixed nuclei. The nuclei were stabilized by spermine. A simple procedure for long‐term storage of samples at —80°C was integrated into the method. The fluorescence of the nuclei was stable for at least 3 hours after staining. Light exposure protection of the samples was essential. No cell loss was caused by storage or staining. The method was successfully applied on samples including: (a) Normal tissues— human lymphocytes, granulocytes and spleen. Mouse lymphocytes, bone marrow, spleen, liver, kidney and thymus. (b) Human neoplasms— lung cancer, breast cancer, lymphoma, leukemia, bladder cancer and cancer of the oral cavity. (c) Human tumors in nude mice— breast cancer, lung cancer, melanoma and colon cancer. (d) Mouse ascites tumors— JB‐1, L 1210, Ehrlich and P 383. It therefore seems well suited as a routine clinical procedure.
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Cites
A fluorescent complex between ethidium bromide and nucleic acids
Pecq, J. B. L., & Paoletti, C. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90353-1 · Cited by 2,393
Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.
Krishan, A. (1975) · The Journal of cell biology · doi:10.1083/jcb.66.1.188 · Cited by 1,728
Separation of Blood Leucocytes, Granulocytes and Lymphocytes
Bøyum, A. (1974) · Tissue Antigens · doi:10.1111/j.1399-0039.1974.tb00252.x · Cited by 663
Vindeløv, L. L. (1977) · Virchows Archiv B Cell Pathology · doi:10.1007/bf02889282 · Cited by 392
Self-citationVindeløv, L. L., Christensen, I. J., & Nissen, N. I. (1983) · Cytometry · doi:10.1002/cyto.990030504 · Cited by 385
Self-citationVindeløv, L. L., Hansen, H. H., Christensen, I. J., Spang‐Thomsen, M., Hirsch, F. R., Hansen, M., & Nissen, N. I. (1980) · PubMed · Cited by 174
Self-citationBICHEL, P., Frederiksen, P., Kjær, T., Thommesen, P., & Vindeløv, L. L. (1977) · Cancer · doi:10.1002/1097-0142(197709)40:3<1206::aid-cncr2820400334>3.0.co;2-z · Cited by 77
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Lee, H.-W., Blasco, M. A., Gottlieb, G. J., Horner, J. W., Greider, C. W., & DePinho, R. A. (1998) · Nature · doi:10.1038/33345 · Cited by 1,270
P53 expression in breast cancer
Cattoretti, G., Rilke, F., Andreola, S., D'Amato, L., & Delia, D. (1988) · International Journal of Cancer · doi:10.1002/ijc.2910410204 · Cited by 601
Cytoplasmic Sequestration of Wild-Type p53 Protein Impairs the G 1 Checkpoint after DNA Damage
Moll, U. M., Ostermeyer, A. G., Haladay, R., Winkfield, B., Frazier, M., & Zambetti, G. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.3.1126 · Cited by 277
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Metadata
- Authors
- Vindeløv, L. L., Christensen, I. J., & Nissen, N. I.
- Venue
- Cytometry
- Publisher
- Wiley
- Published
- 1983-03
- Volume
- 3
- Issue
- 5
- Pages
- 323-327
- ISSN
- 0196-4763 (ISSN-L)
- 1097-0320
- OpenAlex
- W1977379434
- Cited by
- 1,639
- References
- 7
- FWCI
- 26.05
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Vindeløv, L. L., Christensen, I. J., & Nissen, N. I. (1983). A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry. https://doi.org/10.1002/cyto.990030503
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