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Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.
Open accessAbstract
A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.
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Cited by
A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysis
Vindeløv, L. L., Christensen, I. J., & Nissen, N. I. (1983) · Cytometry · doi:10.1002/cyto.990030503 · Cited by 1,639
Yeo, G. W., Coufal, N. G., Liang, T. Y., Peng, G. E., Fu, X.-D., & Gage, F. H. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1545 · Cited by 572
The mitochondrial inner membrane protein mitofilin controls cristae morphology
John, G. B., Shang, Y., Li, L., Renken, C., Mannella, C. A., Selker, J. M. L., Rangell, L., Bennett, M. J., & Zha, J. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-08-0697 · Cited by 440
Goossens, T., Klein, U., & Küppers, R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.5.2463 · Cited by 342
Groshong, S. D., Owen, G. I., Grimison, B., Schauer, I. E., Todd, M. C., Langan, T. A., Sclafani, R. A., Lange, C. A., & Horwitz, K. B. (1997) · Molecular Endocrinology · doi:10.1210/me.11.11.1593 · Cited by 299
Metadata
- Authors
- Krishan, A.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1975-07-01
- Volume
- 66
- Issue
- 1
- Pages
- 188-193
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2169790726
- Cited by
- 1,728
- References
- 0
- FWCI
- 29.61
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Krishan, A. (1975). Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. The Journal of cell biology. https://doi.org/10.1083/jcb.66.1.188
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
