Sensitivity measurement and compensation in spectral imaging
Open accessAbstract
Background: The ImageStream system combines advances in CCD technologies with a novel optical architecture for high sensitivity and multispectral imaging of cells in flow. The sensitivity and dynamic range as well as a methodology for spectral compensation of imagery is presented. Methods: Multicolored fluorescent beads were run on the ImageStream and a flow cytometer. Four single color fluorescent control samples of cells were run to quantify spectral overlap. An additional sample, labeled with all colors was run and compensated in six spectral channels. Results: Analysis of empirical data for sensitivity and dynamic range matched theoretical predictions. The ImageStream system demonstrated fluorescence sensitivity comparable to a PMT‐based flow cytometer. A methodology for addressing spectral overlap, individual pixel anomalies, and multiple imaging modalities was demonstrated for spectral compensation of K562 cells. Imagery is shown pre‐ and post‐compensation. Conclusions: Unlike intensity measurements made with conventional flow cytometers, object size impacts both dynamic range and fluorescence sensitivity in systems that utilize pixilated detection. Simultaneous imaging of alternate modalities can be employed to increase fluorescent sensitivity. Effective compensation of complex multimode imagery spanning six spectral bands is accomplished in a semi‐automated manner. © 2006 International Society for Analytical Cytology
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Single color controls for each fluorochrome were acquired to generate the compensation matrix that was applied to each sample prior to analysis.
“Single color controls for each fluorochrome were acquired to generate the compensation matrix that was applied to each sample prior to analysis (63).”
From Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i> (2024) · cites this paper as [63]
Cites
Golub, T. R., Slonim, D. K., Tamayo, P., Huard, C., Gaasenbeek, M., Mesirov, J. P., Coller, H., Loh, M. L., Downing, J. R., Caligiuri, M. A., et al. (1999) · Science · doi:10.1126/science.286.5439.531 · Cited by 11,516 · see citing works
Shapiro, H. M. (2003) · Wiley eBooks · doi:10.1002/0471722731 · Cited by 2,698 · see citing works
Russ, J. C. (2006) · CRC Press eBooks · doi:10.1201/9780203881095 · Cited by 2,113 · see citing works
Russ, J. C., & Russ, J. C. (2002) · CRC Press eBooks · doi:10.1201/9781420040760 · Cited by 1,385 · see citing works
Shape Analysis and Classification
Costa, L. D. F., & Junior, R. M. C. (2010) · CRC Press eBooks · doi:10.1201/9781420037555 · Cited by 703 · see citing works
Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats
Roederer, M. (2001) · Cytometry · doi:10.1002/1097-0320(20011101)45:3 · Cited by 561 · see citing works
CCD arrays, cameras and displays
Holst, G. C. (1996) · Cited by 498 · see citing works
Borek, F. (1986) · Journal of Immunological Methods · doi:10.1016/0022-1759(86)90397-2 · Cited by 381 · see citing works
The Digital signal processing handbook
(1998) · Choice Reviews Online · doi:10.5860/choice.36-0368 · Cited by 366 · see citing works
The Digital Signal Processing Handbook
Madisetti, V. K. (2009) · doi:10.1201/9781482275193 · Cited by 349 · see citing works
Inoué, S. (1986) · Springer eBooks · doi:10.1007/978-1-4757-6925-8 · Cited by 343 · see citing works
Inoué, S., & Spring, K. R. (1997) · doi:10.1007/978-1-4615-5859-0 · Cited by 326 · see citing works
Video Microscopy: The Fundamentals
Inoué, S., & Spring, K. R. (1986) · Medical Entomology and Zoology · Cited by 304 · see citing works
George, T. C., Fanning, S. L., Fitzgerald-Bocarsly, P. A., Medeiros, R. B., Highfill, S., Shimizu, Y., Hall, B. E., Frost, K., Basiji, D. A., Ortyn, W. E., et al. (2006) · Journal of Immunological Methods · doi:10.1016/j.jim.2006.01.018 · Cited by 251 · see citing works
Self-citationDistinguishing modes of cell death using the ImageStream® multispectral imaging flow cytometer
George, T. C., Basiji, D. A., Hall, B. E., Lynch, D. H., Ortyn, W. E., Perry, D. J., Seo, M. J., Zimmerman, C. A., & Morrissey, P. (2004) · Cytometry Part A · doi:10.1002/cyto.a.20048 · Cited by 250 · see citing works
Self-citationImage and Video Compression for Multimedia Engineering
Shi, Y. Q., & Sun, H. (1999) · Image processing series · doi:10.1201/9781420049794 · Cited by 246 · see citing works
Brennan, K. F. (1999) · Cambridge University Press eBooks · doi:10.1017/cbo9781139164214 · Cited by 201 · see citing works
(2005) · Choice Reviews Online · doi:10.5860/choice.42-5924 · Cited by 158 · see citing works
Practical algorithms for image analysis : description, examples, and code
Seul, M., O’Gorman, L., & Sammon, M. J. (2000) · Cited by 144 · see citing works
Cell Structure and Function by Microspectrofluorometry
(1989) · Elsevier eBooks · doi:10.1016/c2013-0-10983-x · Cited by 106 · see citing works
The Physics of Semiconductors: With Applications to Optoelectronic Devices
Brennan, K. F. (1999) · Cited by 105 · see citing works
Resolution of dimly fluorescent particles: A practical measure of fluorescence sensitivity
Chase, E. S., & Hoffman, R. A. (1998) · Cytometry · doi:10.1002/(sici)1097-0320(19981001)33:2 · Cited by 81 · see citing works
Scientific Photography and Applied Imaging
Ray, S. F. (1999) · Routledge eBooks · doi:10.4324/9780080516707 · Cited by 67 · see citing works
A Practical Guide to CCD Astronomy
Martínez, P., Klotz, A., & Lena, P. J. (1997) · Cambridge University Press eBooks · doi:10.1017/cbo9780511600197 · Cited by 59 · see citing works
Cutting Edge: FADD Is Not Required for Antigen Receptor-Mediated NF-κB Activation
Arechiga, A. F., Bell, B. D., Solomon, J., Chu, I. H., Dubois, C. L., Hall, B. E., George, T. C., Coder, D. M., & Walsh, C. M. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.12.7800 · Cited by 39 · see citing works
Self-citationReeder, J. E., O’Connell, M. J., Yang, Z., Morreale, J. F., Collins, L. L., Frank, I. N., Messing, E. M., Cockett, A. T. �., Cox, C., Robinson, R. D., et al. (1998) · Urology · doi:10.1016/s0090-4295(98)00067-3 · Cited by 30 · see citing works
Sopper, S. (1997) · doi:10.1002/(sici)1097-0320(19971201)29:4
Flagged first, then by citations
3 of the 30 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam‐Klages, S., Agace, W. W., Aghaeepour, N., Akdiş, M., Allez, M., et al. (2019) · European Journal of Immunology · doi:10.1002/eji.201970107 · Cited by 982 · see citing works
The Methods of Choice for Extracellular Vesicles (EVs) Characterization
Szatanek, R., Baj‐Krzyworzeka, M., Zimoch, J., Lekka, M., Siedlar, M., & Baran, J. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18061153 · Cited by 571 · see citing works
Cellular Image Analysis and Imaging by Flow Cytometry
Basiji, D. A., Ortyn, W. E., Liang, L., Vidya Venkatachalam, A., & Morrissey, P. (2007) · Clinics in Laboratory Medicine · doi:10.1016/j.cll.2007.05.008 · Cited by 427 · see citing works
Self-citationAnalytical challenges of extracellular vesicle detection: A comparison of different techniques
Erdbrügger, U., & Lannigan, J. A. (2015) · Cytometry Part A · doi:10.1002/cyto.a.22795 · Cited by 230 · see citing works
Barteneva, N. S., Fasler‐Kan, E., & Vorobjev, I. A. (2012) · Journal of Histochemistry & Cytochemistry · doi:10.1369/0022155412453052 · Cited by 182 · see citing works
Erdbrügger, U., Rudy, C. K., Etter, M. E., Dryden, K. A., Yeager, M. J., Klibanov, A. L., & Lannigan, J. A. (2014) · Cytometry Part A · doi:10.1002/cyto.a.22494 · Cited by 180 · see citing works
Rees, P. C. T., Summers, H. D., Filby, A. J., Carpenter, A. E., & Doan, M. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00167-x · Cited by 180 · see citing works
Mastoridis, S., Bertolino, G. M., Whitehouse, G. P., Dazzi, F., Fueyo, A. S., & Martínez‐Llordella, M. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01583 · Cited by 138 · see citing works
Microscopic imaging techniques for drug discovery
Bullen, A. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2446 · Cited by 138 · see citing works
Multispectral imaging of hematopoietic cells: Where flow meets morphology
McGrath, K. E., Bushnell, T. P., & Palis, J. (2008) · Journal of Immunological Methods · doi:10.1016/j.jim.2008.04.012 · Cited by 130 · see citing works
Zuba‐Surma, E. K., Kucia, M., Wu, W., Klich, I., Lillard, J. W., Ratajczak, J., & Ratajczak, M. Z. (2008) · Cytometry Part A · doi:10.1002/cyto.a.20667 · Cited by 128 · see citing works
The ImageStream System: a key step to a new era in imaging.
Zuba‐Surma, E. K., Kucia, M., Abdel‐Latif, A., Lillard, J. W., & Ratajczak, M. Z. (2007) · PubMed · doi:10.5603/4500 · Cited by 124 · see citing works
McGrath, K. E., Kingsley, P. D., Koniski, A. D., Porter, R. L., Bushnell, T. P., & Palis, J. (2007) · Blood · doi:10.1182/blood-2007-08-107581 · Cited by 122 · see citing works
SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation
Hu, S., Li, J., Xu, F., Mei, S., Le Duff, Y., Yin, L., Pang, X., Cen, S., Jin, Q., Liang, C., et al. (2015) · PLOS Genetics · doi:10.1371/journal.pgen.1005367 · Cited by 121 · see citing works
Flow Cytometry of Extracellular Vesicles: Potential, Pitfalls, and Prospects
Nolan, J. P. (2015) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy1314s73 · Cited by 113 · see citing works
García-Vallejo, J. J., Ilarregui, J. M., Kalay, H., Chamorro, S., Koning, N., Unger, W. W. J., Ambrosini, M., Montserrat, V., Fernandes, R. J., Bruijns, S. C. M., et al. (2014) · The Journal of Experimental Medicine · doi:10.1084/jem.20122192 · Cited by 109 · see citing works
Multivalent glycopeptide dendrimers for the targeted delivery of antigens to dendritic cells
García-Vallejo, J. J., Ambrosini, M., Overbeek, A., van Riel, W. E., Bloem, K., Unger, W. W. J., Chiodo, F., Bolscher, J. G. M., Nazmi, K., Kalay, H., et al. (2012) · Molecular Immunology · doi:10.1016/j.molimm.2012.09.012 · Cited by 109 · see citing works
Zuba‐Surma, E. K., Kucia, M., Abdel‐Latif, A., Dawn, B., Hall, B. E., Singh, R., Lillard, J. W., & Ratajczak, M. Z. (2007) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2007.00154.x · Cited by 109 · see citing works
Self-citation“Small stem cells” in adult tissues: Very small embryonic‐like stem cells stand up!
Zuba‐Surma, E. K., Kucia, M., Ratajczak, J., & Ratajczak, M. Z. (2008) · Cytometry Part A · doi:10.1002/cyto.a.20665 · Cited by 107 · see citing works
Ratajczak, M. Z., Zuba‐Surma, E. K., Shin, D., Ratajczak, J., & Kucia, M. (2008) · Experimental Gerontology · doi:10.1016/j.exger.2008.06.002 · Cited by 105 · see citing works
Imaging flow cytometry for the characterization of extracellular vesicles
Lannigan, J. A., & Erdbrüegger, U. (2016) · Methods · doi:10.1016/j.ymeth.2016.09.018 · Cited by 105 · see citing works
Zucker, R. M., Daniel, K. M., Massaro, E. J., Karafas, S. J., Degn, L. L., & Boyes, W. K. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22342 · Cited by 101 · see citing works
Ratajczak, M. Z., Zuba‐Surma, E. K., Machaliński, B., Ratajczak, J., & Kucia, M. (2008) · Stem Cell Reviews and Reports · doi:10.1007/s12015-008-9018-0 · Cited by 101 · see citing works
Overview of High‐Throughput Screening
Entzeroth, M., Flotow, H., & Condron, P. (2009) · Current Protocols in Pharmacology · doi:10.1002/0471141755.ph0904s44 · Cited by 100 · see citing works
HIV-1 Nef Inhibits Ruffles, Induces Filopodia, and Modulates Migration of Infected Lymphocytes
Nobile, C., Rudnicka, D., Hasan, M., Aulner, N., Porrot, F., Machu, C., Renaud, O., Prévost, M., Hivroz, C., Schwartz, O., et al. (2009) · Journal of Virology · doi:10.1128/jvi.02230-09 · Cited by 100 · see citing works
Flow cytometry for drug discovery, receptor pharmacology and high-throughput screening
Sklar, L. A., Carter, M. B., & EDWARDS, B. (2007) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2007.06.006 · Cited by 86 · see citing works
Extended depth of field imaging for high speed cell analysis
Ortyn, W. E., Perry, D. J., Vidya Venkatachalam, A., Liang, L., Hall, B. E., Frost, K., & Basiji, D. A. (2007) · Cytometry Part A · doi:10.1002/cyto.a.20370 · Cited by 85 · see citing works
Self-citationFilby, A. J., Perucha, E., Summers, H. D., Rees, P. C. T., Chana, P. S., Heck, S., Lord, G. M., & Davies, D. C. (2011) · Cytometry Part A · doi:10.1002/cyto.a.21091 · Cited by 77 · see citing works
Neff‐LaFord, H. D., Teske, S., Bushnell, T. P., & Lawrence, B. P. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.1.247 · Cited by 71 · see citing works
Temporal Dissection of T-bet Functions
Matsuda, J. L., George, T. C., Hagman, J. R., & Gapin, L. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.6.3457 · Cited by 71 · see citing works
Self-citationBeum, P. V., Lindorfer, M. A., Hall, B. E., George, T. C., Frost, K., Morrissey, P., & Taylor, R. P. (2006) · Journal of Immunological Methods · doi:10.1016/j.jim.2006.09.012 · Cited by 68 · see citing works
Self-citationPrinciples of Amnis Imaging Flow Cytometry
Basiji, D. A. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3302-0_2 · Cited by 67 · see citing works
Self-citationMonitoring Extracellular Vesicle Cargo Active Uptake by Imaging Flow Cytometry
Ofir‐Birin, Y., Karam, P. A., Rudik, A., Giladi, T., Porat, Z., & Regev‐Rudzki, N. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01011 · Cited by 65 · see citing works
Calvert, M., Lannigan, J. A., & Pemberton, L. F. (2008) · Cytometry Part A · doi:10.1002/cyto.a.20609 · Cited by 64 · see citing works
Glioma Stem Cells: Novel Data Obtained by Single-Cell Sequencing
Gisina, A. M., Kholodenko, I. V., Kim, Y. S., Abakumov, M. A., Lupatov, A. Y., & Yarygin, K. N. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232214224 · Cited by 63 · see citing works
Flow Cytometry: Impact on Early Drug Discovery
Edwards, B. S., & Sklar, L. A. (2015) · SLAS DISCOVERY · doi:10.1177/1087057115578273 · Cited by 61 · see citing works
da Silva, D. D., Carmo, H., Lynch, A. E., & Silva, E. A. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1082-9 · Cited by 59 · see citing works
Correlating light scattering with internal cellular structures
Marina, O. C., Sanders, C. K., & Mourant, J. R. (2012) · Biomedical Optics Express · doi:10.1364/boe.3.000296 · Cited by 59 · see citing works
Basiji, D. A., & O’Gorman, M. R. G. (2015) · Journal of Immunological Methods · doi:10.1016/j.jim.2015.07.002 · Cited by 59 · see citing works
Self-citationGlycodendrimers prevent HIV transmission via DC-SIGN on dendritic cells
García-Vallejo, J. J., Koning, N., Ambrosini, M., Kalay, H., Vuist, I. M., Sarrami‐Forooshani, R., Geijtenbeek, T. B. H., & van Kooyk, Y. (2013) · International Immunology · doi:10.1093/intimm/dxs115 · Cited by 58 · see citing works
Heterogeneous sensitivity of human acute myeloid leukemia to β-catenin down-modulation
Gandillet, A., Park, S., Lassailly, F., Griessinger, E., Vargaftig, J., Filby, A. J., Lister, T. A., & Bonnet, D. A. (2011) · Leukemia · doi:10.1038/leu.2011.17 · Cited by 55 · see citing works
Wang, J., Gigliotti, F., Bhagwat, S. P., George, T. C., & Wright, T. W. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1001058 · Cited by 54 · see citing works
Self-citationChaar, V., Picot, J., Renaud, O., Bartolucci, P., Nzouakou, R., Bachir, D., Galactéros, F., Colin, Y., Kim, C. L. V., & Nemer, W. E. (2010) · Haematologica · doi:10.3324/haematol.2010.026294 · Cited by 53 · see citing works
Analytical Capabilities of the ImageStream Cytometer
Zuba‐Surma, E. K., & Ratajczak, M. Z. (2011) · Methods in cell biology · doi:10.1016/b978-0-12-374912-3.00008-0 · Cited by 51 · see citing works
Mammary epithelial cells have lineage-rooted metabolic identities
Mahendralingam, M. J., Kim, H., McCloskey, C. W., Aliar, K., Casey, A. E., Tharmapalan, P., Pellacani, D., Ignatchenko, V., García-Valero, M., Palomero, L., et al. (2021) · Nature Metabolism · doi:10.1038/s42255-021-00388-6 · Cited by 48 · see citing works
Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches
Voronin, D. V., Kozlova, A. A., Verkhovskii, R. A., Ermakov, A. V., Makarkin, M. A., Inozemtseva, O. A., & Bratashov, D. N. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21072323 · Cited by 48 · see citing works
Very Small Embryonic-Like Stem Cells: Biology and Therapeutic Potential for Heart Repair
Zuba‐Surma, E. K., Wojakowski, W., Ratajczak, M. Z., & Dawn, B. (2011) · Antioxidants and Redox Signaling · doi:10.1089/ars.2010.3817 · Cited by 46 · see citing works
Soh, K. T., Tario, J. D., Hahn, T., Hillengaß, J., McCarthy, P. L., & Wallace, P. K. (2020) · Cytometry Part B Clinical Cytometry · doi:10.1002/cyto.b.21961 · Cited by 45 · see citing works
Flow Cytometry in Phytoplankton Research
Sosik, H. M., Olson, R. J., & Armbrust, E. V. (2010) · Springer eBooks · doi:10.1007/978-90-481-9268-7_8 · Cited by 45 · see citing works
Ribeiro-Viana, R., García-Vallejo, J. J., Daniel, C., Pérez‐Inestrosa, E., Bloem, K., van Kooyk, Y., & Rojo, J. (2012) · Biomacromolecules · doi:10.1021/bm300998c · Cited by 42 · see citing works
Megjugorac, N. J., Jacobs, E. S., Izaguirre, A. G., George, T. C., Gupta, G., & Fitzgerald-Bocarsly, P. A. (2007) · Immunological Investigations · doi:10.1080/08820130701715845 · Cited by 42 · see citing works
Self-citationBuckman, C., George, T. C., Friend, S. L., Sutovsky, M., Miranda‐Vizuete, A., Ozanon, C., Morrissey, P., & Šutovský, P. (2009) · Systems Biology in Reproductive Medicine · doi:10.3109/19396360903056224 · Cited by 36 · see citing works
Self-citationAutomated identification of circulating tumor cells by image cytometry
Scholtens, T. M., Schreuder, F., Ligthart, S. T., Swennenhuis, J. F., Greve, J., & Terstappen, L. W. M. M. (2011) · Cytometry Part A · doi:10.1002/cyto.a.22002 · Cited by 36 · see citing works
Furia, L., Pelicci, P. G., & Faretta, M. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22266 · Cited by 36 · see citing works
Reporting imaging flow cytometry data for publication: Why mask the detail?
Filby, A. J., & Davies, D. C. (2012) · Cytometry Part A · doi:10.1002/cyto.a.22091 · Cited by 35 · see citing works
CellTracks TDI: An image cytometer for cell characterization
Scholtens, T. M., Schreuder, F., Ligthart, S. T., Swennenhuis, J. F., Tibbe, A. G. J., Greve, J., & Terstappen, L. W. M. M. (2011) · Cytometry Part A · doi:10.1002/cyto.a.21024 · Cited by 33 · see citing works
Human CD127 negative ILC2s show immunological memory
Mathä, L., Krabbendam, L., Martínez-Høyer, S., Heesters, B. A., Golebski, K., Kradolfer, C. M. A., Ghaedi, M., Ma, J., Stadhouders, R., Bachert, C., et al. (2024) · The Journal of Experimental Medicine · doi:10.1084/jem.20231827 · Cited by 29 · see citing works
Liu, Y., Lee, J. J., Lai, T., Lee, C.-H., Hsiao, Y., Chen, P., Liu, W., Hong, C., Lin, S., Kuo, M. Y., et al. (2015) · Head & Neck · doi:10.1002/hed.24034 · Cited by 29 · see citing works
Applications of flow cytometry for measurement of autophagy
Demishtein, A., Porat, Z., Elazar, Z., & Shvets, E. (2015) · Methods · doi:10.1016/j.ymeth.2014.12.020 · Cited by 29 · see citing works
Genotoxic capacity of Cd/Se semiconductor quantum dots with differing surface chemistries
Manshian, B. B., Soenen, S. J., Brown, A., Hondow, N., Wills, J. W., Jenkins, G. J. S., & Doak, S. H. (2015) · Mutagenesis · doi:10.1093/mutage/gev061 · Cited by 29 · see citing works
ImageStream cytometry extends the analysis of phagocytosis and oxidative burst
Ploppa, A., George, T. C., Unertl, K. E., Nohé, B., & Durieux, M. E. (2011) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365513.2011.572182 · Cited by 29 · see citing works
Self-citation“Immuno‐flowFISH” for the Assessment of Cytogenetic Abnormalities in Chronic Lymphocytic Leukemia
Hui, H. Y. L., Clarke, K. M., Fuller, K. A., Stanley, J., Chuah, H. S., Ng, T. F., Cheah, C. Y., McQuillan, A., & Erber, W. N. (2019) · Cytometry Part A · doi:10.1002/cyto.a.23769 · Cited by 29 · see citing works
Retinoic acid induces nuclear accumulation of Raf1 during differentiation of HL-60 cells
Smith, J., Bunaciu, R. P., Reiterer, G., Coder, D. M., George, T. C., Asaly, M., & Yen, A. (2009) · Experimental Cell Research · doi:10.1016/j.yexcr.2009.03.004 · Cited by 27 · see citing works
Self-citationWilliams, M. A., Bauer, S. M., Lu, W., Guo, J., Walter, S., Bushnell, T. P., Lillehoj, E. P., & Georas, S. N. (2009) · Journal of Innate Immunity · doi:10.1159/000254790 · Cited by 27 · see citing works
Ryan, E. P., Bushnell, T. P., Friedman, A. E., Rahman, I., & Phipps, R. P. (2007) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-007-0374-4 · Cited by 27 · see citing works
Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA ‐ DR expression
Connaughton, E. P., Naicker, S. D., Hanley, S. A., Slevin, S. M., Eykelenboom, J. K., Lowndes, N. F., O’Brien, T., Ceredig, R. H., Griffin, M. D., & Dennedy, M. C. (2018) · Immunology and Cell Biology · doi:10.1111/imcb.12032 · Cited by 26 · see citing works
An Imaging Flow Cytometry-based approach to analyse the fission yeast cell cycle in fixed cells
Patterson, J. O., Swaffer, M. P., & Filby, A. J. (2015) · Methods · doi:10.1016/j.ymeth.2015.04.026 · Cited by 24 · see citing works
Effects of acetic acid on light scattering from cells
Marina, O. C., Sanders, C. K., & Mourant, J. R. (2012) · Journal of Biomedical Optics · doi:10.1117/1.jbo.17.8.085002 · Cited by 22 · see citing works
Wabnitz, G. H., Nessmann, A., Kirchgessner, H., & Samstag, Y. (2015) · Journal of Immunological Methods · doi:10.1016/j.jim.2015.03.003 · Cited by 21 · see citing works
Multispectral flow cytometry: The consequences of increased light collection
Feher, K., von Volkmann, K., Kirsch, J., Radbruch, A., Popien, J., & Kaiser, T. (2016) · Cytometry Part A · doi:10.1002/cyto.a.22888 · Cited by 21 · see citing works
Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i>
Louvet, M., Li, J., Brandalise, D., Bachmann, D., Sala de Oyanguren, F., Labes, D., Jacquier, N., Genoud, C., Mucciolo, A., Coste, A. T., et al. (2024) · Microbiology Spectrum · doi:10.1128/spectrum.01531-24 · Cited by 20 · see citing works
Imaging Flow Cytometry to Study Biofilm-Associated Microbial Aggregates
Konieczny, M., Rhein, P., Czaczyk, K., Białas, W., & Juzwa, W. (2021) · Molecules · doi:10.3390/molecules26237096 · Cited by 20 · see citing works
Dynamic intercellular redistribution of HIT antigen modulates heparin-induced thrombocytopenia
Dai, J., Madeeva, D. V., Hayes, V., Ahn, H.-S., Tutwiler, V. J., Arepally, G. M., Cines, D. B., Poncz, M., & Rauova, L. (2018) · Blood · doi:10.1182/blood-2018-02-830737 · Cited by 19 · see citing works
Ruiz-Rodríguez, A. J., Molina-Vallejo, M., Aznar-Peralta, I., Puga, C. G., García, I. C., González‐Flores, E., Lorente, J. A., Serrano, M. J., & Garrido-Navas, M. C. (2021) · Cancers · doi:10.3390/cancers13246386 · Cited by 19 · see citing works
The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry
Filby, A. J., Day, W. P., Purewal, S., & Martínez, N. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3302-0_5 · Cited by 18 · see citing works
Zuba‐Surma, E. K., Kucia, M., & Ratajczak, M. Z. (2007) · Open Life Sciences · doi:10.2478/s11535-007-0044-8 · Cited by 15 · see citing works
Balta, E., Stopp, J., Castelletti, L., Kirchgessner, H., Samstag, Y., & Wabnitz, G. H. (2016) · Methods · doi:10.1016/j.ymeth.2016.09.013 · Cited by 14 · see citing works
Yeast Cell Cycle Analysis: Combining DNA Staining with Cell and Nuclear Morphology
Calvert, M., & Lannigan, J. A. (2010) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy0932s52 · Cited by 13 · see citing works
Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages
Alfandari, D., Pilo, H. B. A., Karam, P. A., Dagan, O., Joubran, C., Rotkopf, R., Regev‐Rudzki, N., & Porat, Z. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.739628 · Cited by 12 · see citing works
IFN-γ Limits Immunopathogenesis but Delays Fungal Clearance during Pneumocystis Pneumonia
Wang, J., Zhang, Z.-Q., Gigliotti, F., & Wright, T. W. (2023) · The Journal of Immunology · doi:10.4049/jimmunol.2300460 · Cited by 9 · see citing works
Allman, A., Gaudette, B. T., Kelly, S., Alouche, N., Carrington, L. J., Perkey, E., Brandstadter, J. D., Outen, R., Vanderbeck, A., Lederer, K., et al. (2024) · Immunity · doi:10.1016/j.immuni.2024.12.003 · Cited by 9 · see citing works
Differential DNA damage repair and PARP inhibitor vulnerability of the mammary epithelial lineages
Kim, H., Aliar, K., Tharmapalan, P., McCloskey, C. W., Kuttanamkuzhi, A., Grünwald, B. T., Palomero, L., Mahendralingam, M. J., Waas, M., Mer, A. S., et al. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.113256 · Cited by 9 · see citing works
Evaluation and Purchase of an Analytical Flow Cytometer: Some of the Numerous Factors to Consider
Zucker, R. M., & Fisher, N. C. (2013) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy0128s63 · Cited by 9 · see citing works
Measurement of Cytoplasmic to Nuclear Translocation
George, T. C., Morrissey, P., Cui, C., Singh, S., & Fitzgerald-Bocarsly, P. A. (2009) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy0928s47 · Cited by 9 · see citing works
Self-citationÖzgün, A., & Gari̇pcan, B. (2021) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2021.119062 · Cited by 8 · see citing works
Pelicci, S., Furia, L., Pelicci, P. G., & Faretta, M. (2023) · Cells · doi:10.3390/cells12030354 · Cited by 7 · see citing works
Filby, A., Perucha, E., Summers, H., Rees, P. C. T., Chana, P., Heck, S., Lord, G. M., & Davies, D. (2011) · Cytometry Part A · doi:10.1002/cyto.a.v79a.7 · Cited by 7 · see citing works
Łabędź‐Masłowska, A., Kamycka, E., Bobis‐Wozowicz, S., Madeja, Z., & Zuba‐Surma, E. K. (2015) · Stem Cells International · doi:10.1155/2016/5069857 · Cited by 6 · see citing works
Konstantinidis, D. G., Pushkaran, S., Giger, K., Manganaris, S., Zheng, Y., & Kalfa, T. A. (2014) · Journal of Visualized Experiments · doi:10.3791/50990 · Cited by 6 · see citing works
MULTISPECTRAL IMAGING FLOW CYTOMETRY
Basiji, D. A. (2007) · IEEE International Symposium on Biomedical Imaging (ISBI) · doi:10.1109/isbi.2007.357048 · Cited by 6 · see citing works
Self-citationFlow Cytometry Quality Assurance
Zucker, R. M. (2008) · Springer series on fluorescence · doi:10.1007/4243_2008_047 · Cited by 6 · see citing works
Hosseini, E., Pezeshki, S. M. S., Ghasemzadeh, A., & Ghasemzadeh, M. (2025) · Journal of Thrombosis and Thrombolysis · doi:10.1007/s11239-025-03121-2 · Cited by 5 · see citing works
Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
McFarlin, B. K., Venable, A. S., Prado, E., Henning, A. L., & Williams, R. R. (2014) · Journal of Visualized Experiments · doi:10.3791/52201 · Cited by 5 · see citing works
Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry
Vorobjev, I. A., & Barteneva, N. S. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3302-0_16 · Cited by 5 · see citing works
Mammary lineage dictates homologous recombination repair and PARP inhibitor vulnerabilityPreprint
Kim, H., Casey, A. E., Palomero, L., Aliar, K., Mahendralingam, M. J., Parsons, M., Narala, S., Mateo, F., Hofer, S. O. P., Waterhouse, P. D., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.05.14.444217 · Cited by 5 · see citing works
Tauzin, L., Campos, V., & Tichet, M. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23351 · Cited by 4 · see citing works
Laser image cytometer for analysis of circulating tumor cells
Schreuder, F. (2008) · doi:10.3990/1.9789036526104 · Cited by 4 · see citing works
Advances in Optical Technologies for Rare Cell Detection and Characterization
Dietz, L., & Bruce, R. (2012) · Recent results in cancer research/Recent Results in Cancer Research · doi:10.1007/978-3-642-28160-0_7 · Cited by 3 · see citing works
Imaging Flow Cytometry as a Molecular Biology Tool: From Cell Morphology to Molecular Mechanisms
Matsuoka, Y. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26199261 · Cited by 2 · see citing works
Zytomik und prädiktive Medizin in der Onkologie
Gerstner, A. O. H., & Laffers, W. (2008) · HNO · doi:10.1007/s00106-008-1703-4 · Cited by 2 · see citing works
- Authors
- Ortyn, W. E., Hall, B. E., George, T. C., Frost, K., Basiji, D. A., Perry, D. J., Zimmerman, C. A., Coder, D., & Morrissey, P. J.
- Corresponding author
- Ortyn, William E.
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2006-08
- Volume
- 69A
- Issue
- 8
- Pages
- 852-862
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- OpenAlex
- W2149072868
- Cited by
- 115
- References
- 30
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 6 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 1 · 2016: 3 · 2015: 15 · 2014: 5 · 2013: 7 · 2012: 9
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cytometry Part A (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (9 R44 CA01798-02, R44CA001798)
- National Institute of General Medical Sciences (R43GM058956, Q R43 GM58956-01)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Engineering › Electrical and Electronic Engineering › CCD and CMOS Imaging Sensors
- Keywords
- spectral imaging, flow cytometry, fluorescence sensitivity, dynamic range, spectral compensation, spectral overlap, multispectral imaging, K562 cells, fluorescent microspheres, CCD detection, multimodal imaging
- MeSH terms
- Diagnostic Imaging, Flow Cytometry, Fluorescence, Fluorescent Antibody Technique, Humans, Mathematics, Microspheres, Sensitivity and Specificity, Spectrometry, Fluorescence, Spectrum Analysis, Image Cytometry, Cell Line, Tumor
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