A novel flow cytometric protocol for assessment of yeast cell adhesion
Open accessAbstract
Microbial adhesion is a field of recognized relevance and, as such, an impressive array of tools has been developed to understand its molecular mechanisms and ultimately for its quantification. Some of the major limitations found within these methodologies concern the incubation time, the small number of cells analyzed, and the operator's subjectivity. To overcome these aspects, we have developed a quantitative method to measure yeast cells' adhesion through flow cytometry. In this methodology, a suspension of yeast cells is mixed with green fluorescent polystyrene microspheres (uncoated or coated with host proteins). Within 2 h, an adhesion profile is obtained based on two parameters: percentage and cells‐microsphere population's distribution pattern. This flow cytometry protocol represents a useful tool to quantify yeast adhesion to different substrata in a large scale, providing manifold data in a speedy and informative manner. © 2011 International Society for Advancement of Cytometry
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Adhesion to fluorescent polystyrene microspheres as a readout for adhesion to hydrophobic surfaces can be analyzed by flow cytometry.
“Adhesion to fluorescent polystyrene microspheres as a readout for adhesion to hydrophobic surfaces can be analyzed by flow cytometry as described previously (61).”
From Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i> (2024) · cites this paper as [61]
In the flow cytometry protocol, microspheres act as a surface to which yeast cells can adhere, enabling their quantification.
“In this protocol, microspheres act as a surface to which yeast cells can adhere enabling their quantification.”
From Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i> (2024) · cites this paper as [61]
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“(11) using the six-well plate assay method.”
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Santana, D. J., Anku, J. A. E., Zhao, G., Zarnowski, R., Johnson, C. J., Hautau, H., Visser, N. D., Ibrahim, A. S., Andes, D., Nett, J. E., et al. (2023) · Science · doi:10.1126/science.adf8972 · Cited by 142 · see citing works
Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i>
Louvet, M., Li, J., Brandalise, D., Bachmann, D., Sala de Oyanguren, F., Labes, D., Jacquier, N., Genoud, C., Mucciolo, A., Coste, A. T., et al. (2024) · Microbiology Spectrum · doi:10.1128/spectrum.01531-24 · Cited by 20 · see citing works
- Authors
- Silva‐Dias, A., Miranda, I. M., Rocha, R., Monteiro‐Soares, M., Salvador, A., Rodrigues, A. G., & Pina‐Vaz, C.
- Affiliations
- Universidade do Porto (PT)
- Universidade Politécnica de Lisboa (PT)
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2011-11-10
- Volume
- 81A
- Issue
- 3
- Pages
- 265-270
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- OpenAlex
- W1981832826
- Cited by
- 19
- References
- 2
- Citations by year
- 2026: 2 · 2024: 3 · 2023: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2017: 3 · 2016: 1 · 2015: 2 · 2013: 1
- FWCI
- 0.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cytometry Part A (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Funders
- Universidade do Porto
- Fundação para a Ciência e a Tecnologia (Ciência 2008)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- yeast cell adhesion, flow cytometry protocol, cell-substrate adhesion
- MeSH terms
- Candida, Cell Adhesion, Flow Cytometry, Fluorescent Dyes, Microspheres, Saccharomyces cerevisiae
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