The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Long‐term culture of human embryonic stem cells in feeder‐free conditions
Abstract
We have demonstrated previously that human embryonic stem (hES) cells possess a characteristic morphologic, antigenic, and molecular profile that can be used to assess the state of ES cells (Carpenter et al., [] Dev Dyn 229:243–258). In this manuscript, we have examined the long‐term stability of three hES cell lines in feeder‐free culture. We demonstrate that the expression of antigens and transcription factors, telomerase activity, telomere length, and karyotype appear stable for all three hES cell lines after continuous culture for over 1 yr. All three lines retained pluripotent differentiation in vitro and in vivo. Although hES cell lines were remarkably stable over the period of analysis, a detailed quantitative analysis of antigen expression by flow cytometry and gene expression by microarray suggested that cell lines show subtle differences in the expression of small subsets of genes upon long‐term culture. Developmental Dynamics 229:259–274, 2004. © 2003 Wiley‐Liss, Inc.
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Cites
Specific Association of Human Telomerase Activity with Immortal Cells and Cancer
Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., Coviello, G. M., Wright, W. E., Weinrich, S. L., & Shay, J. W. (1994) · Science · doi:10.1126/science.7605428 · Cited by 7,091 · cited 1×
“Telomerase activity was measured using the TRAP assay as described (Kim et al., 1994; Weinrich et al., 1997).”
MethodTelomeres shorten during ageing of human fibroblasts
Harley, C. B., Futcher, A. B., & Greider, C. W. (1990) · Nature · doi:10.1038/345458a0 · Cited by 5,750
Cited by
Kroon, E., Martinson, L. A., Kadoya, K., Bang, A. G., Kelly, O. G., Eliazer, S., Young, H., Richardson, M., Smart, N. G., Cunningham, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1393 · Cited by 1,843
Self-citationHuman embryonic stem cells express an immunogenic nonhuman sialic acid
Martin, M. J., Muotri, A., Gage, F., & Varki, A. (2005) · Nature Medicine · doi:10.1038/nm1181 · Cited by 932
Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
Wobus, A. M., & Boheler, K. R. (2005) · Physiological Reviews · doi:10.1152/physrev.00054.2003 · Cited by 782
Differences between human and mouse embryonic stem cells
Ginis, I., Luo, Y., Miura, T., Thies, S., Brandenberger, R., Gerecht-Nir, S., Amit, M., Hoke, A., Carpenter, M. K., Itskovitz-Eldor, J., et al. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2003.12.034 · Cited by 717
Self-citationInitiative, T. I. S. C. (2011) · Nature Biotechnology · doi:10.1038/nbt.2051 · Cited by 577
Metadata
- Authors
- Rosler, E. S., Fisk, G. J., Ares, X., Irving, J., Miura, T., Rao, M. S., & Carpenter, M. K.
- Venue
- Developmental Dynamics
- Publisher
- Wiley
- Published
- 2003-12-18
- Volume
- 229
- Issue
- 2
- Pages
- 259-274
- ISSN
- 1058-8388 (ISSN-L)
- 1097-0177
- OpenAlex
- W2056707698
- Cited by
- 357
- References
- 2
- FWCI
- 12.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rosler, E. S., Fisk, G. J., Ares, X., Irving, J., Miura, T., Rao, M. S., & Carpenter, M. K. (2004). Long‐term culture of human embryonic stem cells in feeder‐free conditions. Developmental Dynamics. https://doi.org/10.1002/dvdy.10430
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