The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos
Abstract
We have adapted a novel multicistronic gene expression system involving viral peptides to the zebrafish. The viral 2A peptide allows production of multiple protein products from a single transgene. Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA's allows the stoichiometric translation of multiple unfused protein products. To test this system in zebrafish, we generated two different tandem reporter constructs employing eGFP and mCherry, separated by 2A peptide sequence. Using this system, we produced transgenic zebrafish in which fluorophores were produced as independent proteins from a single transcript. The successful application of this technology in zebrafish will be valuable for visually marking transgenic embryos and transgene‐expressing cells, or in any situation where reliable expression of multiple transgenes is desired. genesis 45:625–629, 2007. © 2007 Wiley‐Liss, Inc.
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Mizuguchi, H., Xu, Z., Ishii-Watabe, A., Uchida, E., & Hayakawa, T. (2000) · Molecular Therapy · doi:10.1006/mthe.2000.0050 · Cited by 559
Cited by
Yoo, S. K., Deng, Q., Cavnar, P. J., Wu, Y. I., Hahn, K. M., & Huttenlocher, A. (2010) · Developmental Cell · doi:10.1016/j.devcel.2009.11.015 · Cited by 351
Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo
Czopka, T., ffrench-Constant, C., & Lyons, D. A. (2013) · Developmental Cell · doi:10.1016/j.devcel.2013.05.013 · Cited by 342
Optimized Gal4 genetics for permanent gene expression mapping in zebrafish
Distel, M., Wullimann, M. F., & Köster, R. W. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903060106 · Cited by 289
Eom, D. S., Inoue, S., Patterson, L. B., Gordon, T. N., Slingwine, R., Kondo, S., Watanabe, M., & Parichy, D. M. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002899 · Cited by 88
Eom, D. S., Patterson, L. B., Bostic, R. R., & Parichy, D. M. (2021) · Developmental Biology · doi:10.1016/j.ydbio.2021.04.007 · Cited by 23
Metadata
- Authors
- Provost, E., Rhee, J., & Leach, S. D.
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2007-10
- Volume
- 45
- Issue
- 10
- Pages
- 625-629
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- OpenAlex
- W2124937806
- Cited by
- 189
- References
- 1
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- closed
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