Cloning and expression of an inducible lymphoid‐specific, protein tyrosine phosphatase (HePTPase)
Abstract
Tyrosine phosphorylation and dephosphorylating events have been shown to be central to the process of growth regulation and signal transduction. We report here, the identification of a new gene with a tyrosine phosphatase domain (EC 3.1.3.48) which is expressed exclusively in thymus and spleen. A cDNA of 2760 bp encodes a 339‐amino acid, intracellular, single‐domain tyrosine phosphatase. When expressed as a glutathionine‐S‐transferase fusion protein, efficient lysis of p‐nitrophenyl phosphate is noted, indicating in vitro enzymatic activity of the cloned gene product. Normal mouse lymphocytes increase mRNA expression 10‐15‐fold upon stimulation with phytohemagglutinin, concanavalin A, lipo‐polysaccharide or anti‐CD3 monoclonal antibody. This new hematopoietic tyrosine phosphatase, (HePTP), may play a role in the regulation of T and B lymphocyte development and signal transduction.
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KIM-PTPs HePTP, STEP, and PTPSL possess an N-terminal unstructured extension that includes the KIM, followed by their C-terminal phosphatase domain.
“Furthermore, the KIM-PTPs [Fig.1(B), center; hematopoietic tyrosine phosphatase, HePTP, immune system specific; 25 striatum-enriched phosphatase, STEP, brain specific; 26 STEP-like PTP, PTPSL, brain specific 27 ) also possess an N-terminal unstructured extension, which includes the KIM, followed by their C-terminal phosphatase domain (PTP).”
From Molecular basis of MAP kinase regulation (2013) · cites this paper as [25]
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- Authors
- Zanke, B., Suzuki, H., Kishihara, K., Mizzen, L., Minden, M., Pawson, A., & Mak, T. W.
- Affiliations
- University of Toronto (CA)
- Mount Sinai Hospital (CA)
- Corresponding author
- Mak, Tak W.
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 1992-01
- Volume
- 22
- Issue
- 1
- Pages
- 235-239
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W2067920491
- Cited by
- 101
- References
- 25
- Citations by year
- 2026: 1 · 2021: 1 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 3
- FWCI
- 2.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- protein tyrosine phosphatase, thymus, spleen, lymphocyte activation, signal transduction, T cell development, B cell development, gene cloning, cDNA expression, GST fusion protein, anti-CD3 stimulation, concanavalin A, phytohemagglutinin, lipopolysaccharide
- MeSH terms
- Amino Acid Sequence, Animals, Antigens, Differentiation, T-Lymphocyte, Base Sequence, Cloning, Molecular, Concanavalin A, Lipopolysaccharides, Lymphocytes, Mice, Inbred BALB C, Molecular Sequence Data, Phytohemagglutinins, Receptors, Antigen, T-Cell, RNA, Messenger, Protein Tyrosine Phosphatases, CD3 Complex, Mice
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