Cited by 51MicroRNA in disease regulationAir Quality and Health ImpactsOccupational and environmental lung diseases

Carbon black nanoparticle intratracheal installation results in large and sustained changes in the expression of miR‐135b in mouse lung

Bourdon, Julie A., Saber, Anne T., Halappanavar, Sabina, Jackson, Petra A., Wu, Dongmei, Hougaard, Karin S., Jacobsen, Nicklas R., Williams, Andrew +3 more·2012·Environmental and Molecular Mutagenesis

Abstract

MicroRNAs (miRNA) are important noncoding regulatory molecules that bind target messenger RNA (mRNA), primarily affecting their translation into protein. Because miRNAs can simultaneously target hundreds of mRNAs, subtle changes in their expression can elicit important cellular effects. Little is known about the role of miRNAs in pulmonary responses to inhaled particulate matter. We studied pulmonary global miRNA responses to Printex 90 carbon black nanoparticles in (1) nonpregnant C57BL/6 female mice instilled with vehicle or a single dose of 0.162 mg and euthanized 1, 3, and 28 days post-exposure, and (2) C57BL/6Bom Tac dams instilled with vehicle or a cumulative dose of 0.268 mg (four separate instillations of vehicle or 0.067 mg Printex 90 during pregnancy) and euthanized at weaning (26-27 days postexposure). We measured similar expression profiles in both exposure scenarios, with marked increases in miR-135b and subtle changes in miR-21 and miR-146b. All three miRNAs were confirmed in nonpregnant females by RT-PCR, whereas only miR-135b was confirmed in the dams. Target analysis revealed no concomitant changes in established and predicted targets of miR-135b, miR-21, or miR-146b. Analysis of potentially perturbed pathways did not reveal changes that would suggest down-stream miRNA effects. The reasons for the lack of association between miRNA and transcript profiles may be related to the complexity of miRNA function and fate, or to the possibility that targets may differ…

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Crossref · 10.1002/em.21706

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Authors
Bourdon, J. A., Saber, A. T., Halappanavar, S., Jackson, P. A., Wu, D., Hougaard, K. S., Jacobsen, N. R., Williams, A., Vogel, U., Wallin, H., & Yauk, C. L.
Affiliations
  • Health Canada (CA)
  • National Research Centre for the Working Environment (DK)
Corresponding author
Yauk, Carole L.
Venue
Environmental and Molecular Mutagenesis
Publisher
Wiley
Published
2012-06-29
Volume
53
Issue
6
Pages
462-468
ISSN
  • 0893-6692 (ISSN-L)
  • 1098-2280
OpenAlex
W2122889851
Cited by
51
References
36
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 8 · 2014: 4 · 2013: 5
FWCI
1.81
Type
journal article
Language
en
Open access
closed
Locations
  • Environmental and Molecular Mutagenesis (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Occupational and environmental lung diseases
Keywords
carbon black nanoparticles, intratracheal instillation, miR-135b, miR-21, miR-146b, microRNA expression, lung inflammation, pregnancy, maternal exposure, inhaled particles, lung transcriptome
MeSH terms
Animals, Female, Intubation, Intratracheal, Lung, Mice, Inbred C57BL, Pregnancy, Gene Expression Profiling, MicroRNAs, Mice, Soot, Nanoparticles

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