Carbon black nanoparticle intratracheal installation results in large and sustained changes in the expression of miR‐135b in mouse lung
Abstract
MicroRNAs (miRNA) are important noncoding regulatory molecules that bind target messenger RNA (mRNA), primarily affecting their translation into protein. Because miRNAs can simultaneously target hundreds of mRNAs, subtle changes in their expression can elicit important cellular effects. Little is known about the role of miRNAs in pulmonary responses to inhaled particulate matter. We studied pulmonary global miRNA responses to Printex 90 carbon black nanoparticles in (1) nonpregnant C57BL/6 female mice instilled with vehicle or a single dose of 0.162 mg and euthanized 1, 3, and 28 days post-exposure, and (2) C57BL/6Bom Tac dams instilled with vehicle or a cumulative dose of 0.268 mg (four separate instillations of vehicle or 0.067 mg Printex 90 during pregnancy) and euthanized at weaning (26-27 days postexposure). We measured similar expression profiles in both exposure scenarios, with marked increases in miR-135b and subtle changes in miR-21 and miR-146b. All three miRNAs were confirmed in nonpregnant females by RT-PCR, whereas only miR-135b was confirmed in the dams. Target analysis revealed no concomitant changes in established and predicted targets of miR-135b, miR-21, or miR-146b. Analysis of potentially perturbed pathways did not reveal changes that would suggest down-stream miRNA effects. The reasons for the lack of association between miRNA and transcript profiles may be related to the complexity of miRNA function and fate, or to the possibility that targets may differ…
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Mir-21 was significantly increased in response to diesel exhaust particle and metal-rich PM exposure in adults.
“Mir-21, which is an important regulator in vascular cell proliferation and apoptosis, was also significantly increased in response to diesel exhaust particle and metal-rich PM exposure in adults [197, 198].”
From Air pollution and children’s health—a review of adverse effects associated with prenatal exposure from fine to ultrafine particulate matter (2021) · cites this paper as [198]
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- Authors
- Bourdon, J. A., Saber, A. T., Halappanavar, S., Jackson, P. A., Wu, D., Hougaard, K. S., Jacobsen, N. R., Williams, A., Vogel, U., Wallin, H., & Yauk, C. L.
- Affiliations
- Health Canada (CA)
- National Research Centre for the Working Environment (DK)
- Corresponding author
- Yauk, Carole L.
- ORCID
- Venue
- Environmental and Molecular Mutagenesis
- Publisher
- Wiley
- Published
- 2012-06-29
- Volume
- 53
- Issue
- 6
- Pages
- 462-468
- ISSN
- 0893-6692 (ISSN-L)
- 1098-2280
- OpenAlex
- W2122889851
- Cited by
- 51
- References
- 36
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 8 · 2014: 4 · 2013: 5
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental and Molecular Mutagenesis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Occupational and environmental lung diseases
- Keywords
- carbon black nanoparticles, intratracheal instillation, miR-135b, miR-21, miR-146b, microRNA expression, lung inflammation, pregnancy, maternal exposure, inhaled particles, lung transcriptome
- MeSH terms
- Animals, Female, Intubation, Intratracheal, Lung, Mice, Inbred C57BL, Pregnancy, Gene Expression Profiling, MicroRNAs, Mice, Soot, Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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