Methylation alterations at imprinted genes detected among long‐term shiftworkers
Open accessAbstract
Exposure to light at night through shiftwork has been linked to alterations in DNA methylation and increased risk of cancer development. Using an Illumina Infinium Methylation Assay, we analyzed methylation levels of 397 CpG sites in the promoter regions of 56 normally imprinted genes to investigate whether shiftwork is associated with alteration of methylation patterns. Methylation was significantly higher at 20 CpG sites and significantly lower at 30 CpG sites (P < 0.05) in 10 female long‐term shiftworkers as compared to 10 female age‐ and folate intake‐matched day workers. The strongest evidence for altered methylation patterns in shiftworkers was observed for DLX5, IGF2AS, and TP73 based on the magnitude of methylation change and consistency in the direction of change across multiple CpG sites, and consistent results were observed using quantitative DNA methylation analysis. We conclude that long‐term shiftwork may alter methylation patterns at imprinted genes, which may be an important mechanism by which shiftwork has carcinogenic potential and warrants further investigation. Environ. Mol. Mutagen., 2013. © 2012 Wiley Periodicals, Inc.
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Hypermethylation of MGMT has been reported to decrease apoptotic responses to DNA damage by TP73 and cell proliferation regulation by RBBP7, which in combination with aberrant expression of HOXA10, increase susceptibility for endometriosis.
“The hypermethylation of methyltransferase (MGMT) has been reported to decrease apoptotic responses to DNA damage by TP73 and cell proliferation regulation by RBBP7, which in combination with the aberrant expression of HOXA10, increase the susceptibility for endometriosis.12,[41][42][43][44][45][46][47][48]”
From Altered genome-wide methylation in endometriosis (2014) · cites this paper as [42]
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- Authors
- Jacobs, D. I., Hansen, J., Fu, A., Stevens, R. G., Tjonneland, A., Vogel, U. B., Zheng, T., & Zhu, Y.
- Affiliations
- Yale University (US)
- Danish Cancer Society (DK)
- UConn Health (US)
- National Research Centre for the Working Environment (DK)
- Corresponding author
- Zhu, Yong
- Venue
- Environmental and Molecular Mutagenesis
- Publisher
- Wiley
- Published
- 2012-11-28
- Volume
- 54
- Issue
- 2
- Pages
- 141-146
- ISSN
- 0893-6692 (ISSN-L)
- 1098-2280
- PMC
- PMC4993623
- OpenAlex
- W2094158947
- Cited by
- 34
- References
- 30
- Citations by year
- 2026: 2 · 2025: 2 · 2022: 1 · 2020: 2 · 2019: 1 · 2017: 8 · 2016: 2 · 2015: 7 · 2014: 5 · 2013: 4
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Environmental and Molecular Mutagenesis (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Environmental Health Sciences (ES018915, R21ES018915)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Keywords
- shift work, DNA methylation, genomic imprinting, imprinted genes, light at night, Dlx5, p73, promoter methylation, CpG sites, cancer risk
- MeSH terms
- Tumor Protein p73, DNA-Binding Proteins, Female, Humans, Light, Middle Aged, Neoplasms, Nuclear Proteins, Promoter Regions, Genetic, Proteins, Surveys and Questionnaires, Risk, Time Factors, Transcription Factors, Work Schedule Tolerance, Genomic Imprinting, Homeodomain Proteins, CpG Islands, DNA Methylation, Tumor Suppressor Proteins, Transcriptome
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