Analysis of engineered nanomaterials in complex matrices (environment and biota): General considerations and conceptual case studies
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Advances in the study of the environmental fate, transport, and ecotoxicological effects of engineered nanomaterials (ENMs) have been hampered by a lack of adequate techniques for the detection and quantification of ENMs at environmentally relevant concentrations in complex media. Analysis of ENMs differs from traditional chemical analysis because both chemical and physical forms must be considered. Because ENMs are present as colloidal systems, their physicochemical properties are dependent on their surroundings. Therefore, the simple act of trying to isolate, observe, and quantify ENMs may change their physicochemical properties, making analysis extremely susceptible to artifacts. Many analytical techniques applied in materials science and other chemical/biological/physical disciplines may be applied to ENM analysis as well; however, environmental and biological studies may require that methods be adapted to work at low concentrations in complex matrices. The most pressing research needs are the development of techniques for extraction, cleanup, separation, and sample storage that introduce minimal artifacts to increase the speed, sensitivity, and specificity of analytical techniques, as well as the development of techniques that can differentiate between abundant, naturally occurring particles, and manufactured nanoparticles.
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The ability of instrumental analysis techniques to generate large volumes of information creates a need to harmonize such information into a meaningful explanation.
“The ability of instrumental analysis techniques such as chromatography, spectroscopy, spectrophotometry and microscopy to generate large volumes of information, even from a single chemical or environmental sample creates a need to harmonize such information into a meaningful explanation [59,60,61].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [61]
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BackgroundEnvironmental concentrations of engineered nanomaterials: Review of modeling and analytical studies
Gottschalk, F., Sun, T., & Nowack, B. (2013) · Environmental Pollution · doi:10.1016/j.envpol.2013.06.003 · Cited by 1,135 · see citing works
Strong Sorption of PCBs to Nanoplastics, Microplastics, Carbon Nanotubes, and Fullerenes
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Comprehensive probabilistic modelling of environmental emissions of engineered nanomaterials
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Toxicity of Engineered Nanoparticles in the Environment
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Nanoplastics in the Aquatic Environment. Critical Review
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“…that apply to nanomaterials in general are also useful for nanoplastic fate and effect research, such as UV-VIS spectrometry, electron microscopy, field flow fractionation (FFF) or dynamic light scattering (DLS) techniques, each having their advantages and flaws (Von der Kammer et al. 2012).”
BackgroundNanopesticides: State of Knowledge, Environmental Fate, and Exposure Modeling
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Potential scenarios for nanomaterial release and subsequent alteration in the environment
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Effects of nanopolystyrene on the feeding behavior of the blue mussel (<i>Mytilus edulis</i> L.)
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Dynamic Probabilistic Modeling of Environmental Emissions of Engineered Nanomaterials
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Single Particle Inductively Coupled Plasma Mass Spectrometry: A Powerful Tool for Nanoanalysis
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Nanopesticides: Guiding Principles for Regulatory Evaluation of Environmental Risks
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Fate and Bioavailability of Engineered Nanoparticles in Soils: A Review
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“Analyzing extracts can benefit from the rapidly developing technology to analyze very low ENM concentrations in complex fluids (von der Kammer et al., 2012) and ENM occurs from the soil pore solution as altered by nearby organisms, e.g., in the rhizosphere or the invertebrate gut.”
BackgroundRisks, Release and Concentrations of Engineered Nanomaterial in the Environment
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Nanoparticles in food packaging: Biodegradability and potential migration to food—A review
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Paradigms to assess the environmental impact of manufactured nanomaterials
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“Indeed, many of the challenges for water samples are on the verge of being overcome, although the techniques are still far from routine applications [25].”
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Modeling Nanomaterial Environmental Fate in Aquatic Systems
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Characterization of Nanomaterials
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“A major hurdle is clearly that analytical methods are missing so far that could speci fi cally and quantitatively identify and characterize the released CNTs under real-world conditions (von der Kammer et al., 2012).”
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“An 274 additional challenge, which may be relevant for both ENPs and MPs is the fact that laboratory 275 experiments typically require the use of particle concentrations that are orders of magnitude greater 276 than those likely to be found in natural environments,(12) because the detection limits of currently 277 ava…”
BackgroundMethodInfluentialHandy, R. D., van den Brink, N. W., Chappell, M. A., Mühling, M., Behra, R., Dušinská, M., Simpson, P., Ahtiainen, J., Jha, A. N., Seiter, J. M., et al. (2012) · Ecotoxicology · doi:10.1007/s10646-012-0862-y · Cited by 219 · see citing works
“…methodology including the OECD, the International Organisation for Standardization (ISO) and the Society of Environmental Toxicology and Chemistry Nano Advisory Group which has very recently reported (see Handy et al. 2012 on ecotoxicity, and Von der Kammer et al. 2012 on analytical methods).”
BackgroundMethodGray, E. P., Coleman, J. G., Bednar, A. J., Kennedy, A. R., Ranville, J. F., & Higgins, C. P. (2013) · Environmental Science & Technology · doi:10.1021/es403558c · Cited by 217 · see citing works
“light scattering (DLS) [13], though other techniques, such as nanotracking analysis (NTA) or 58 electron microscopy (EM) are also used [14].”
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“From the analytical point of view, there are still many challenges to master before trace analysis of engineered ENM in natural samples is possible, especially with respect to the distinction between natural and engineered nanoparticles (von der Kammer et al., 2012).”
Particle toxicology and health - where are we?
Riediker, M., Zink, D., Kreyling, W. G., Oberdörster, G., Elder, A. C. P., Graham, U. M., Lynch, I., Duschl, A., Ichihara, G., Ichihara, S., et al. (2019) · Particle and Fibre Toxicology · doi:10.1186/s12989-019-0302-8 · Cited by 222 · see citing works
“One of the technical challenges ahead is the development of routine detection methods for NPs in environmental samples [256], and although some methods have been recently developed such as single particle induction couple mass spectrometry sp-ICP-MS, there remains reliance on computational models…”
Multimedia Environmental Distribution of Engineered Nanomaterials
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Legal and practical challenges in classifying nanomaterials according to regulatory definitions
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“Although there already is a great deal of information about the toxic effects of ENMs, very little is known about environmental exposure, mainly because to date there are no available quantitative and specific trace analytical methods for detecting them in the environment [20].”
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“magna and found no evidence that AuNPs were absorbed across epithelial membranes, a result similar to other studies with carbon nanomaterial [23,35,36].”
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Coronas of micro/nano plastics: a key determinant in their risk assessments
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Microplastics and Nanoplastics in Aquatic Environments: Challenges and Threats to Aquatic Organisms
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Low risk posed by engineered and incidental nanoparticles in drinking water
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Biological accumulation of engineered nanomaterials: a review of current knowledge
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Lung Macrophages “Digest” Carbon Nanotubes Using a Superoxide/Peroxynitrite Oxidative Pathway
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“Such an approach allows mapping and quantification of a wide range of nanomaterials inside cells and tissues.(50) Sections were inspectedwith 60 oil immersion objectives.”
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“…allow a general characterisation of NM effects, and verification of the results from such tests taking into consideration environmentally relevant groups of organisms (building block 1b) and realistic environmental exposure scenarios may be necessary (building block 2) (von der Kammer et al. 2012).”
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“, NIST reference materials 8011-8013 [7], ERM-FD100 and ERM-FD304 [8], or BAM-N001 [9]), the validation of analytical methods for ENPs is challenging and often multi-method approaches are required to provide reliable data as well as to assess and control the limitations of different techniques [6, 10, 11].”
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“Detailed information regarding these conventional methods has been recently presented (Farré et al., 2011; von der Kammer et al., 2012).”
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Hazard assessment of a silver nanoparticle in soil applied via sewage sludge
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“The lack of adequate techniques for detection and quantification of nanoparticles at environmentally relevant concentrations in complex media hampers the assessment of the results [28].”
BackgroundModeling nanomaterial fate and uptake in the environment: current knowledge and future trends
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Release of TiO2 from paints containing pigment-TiO2 or nano-TiO2 by weathering
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Detection of Engineered Copper Nanoparticles in Soil Using Single Particle ICP-MS
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“, isotope or elemental ratios, morphology, surface properties) and develop strategies to distinguish between ENPs and natural backgrounds to be able to assess the anthropogenic portion among natural NPs [7,8].”
BackgroundThe Road to Sustainable Nanotechnology: Challenges, Progress and Opportunities
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Hyperspectral microscopy as an analytical tool for nanomaterials
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Analytical characterisation of nanoscale zero-valent iron: A methodological review
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Outdoor urban nanomaterials: The emergence of a new, integrated, and critical field of study
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- Authors
- von der Kammer, F., Ferguson, P. L., Holden, P. A., Masion, A., Rogers, K. R., Klaine, S. J., Koelmans, A. A., Horne, N., & Unrine, J. M.
- Affiliations
- Department of Environmental Geosciences, University of Vienna, Vienna, Austria
- Duke University (US)
- University of California, Santa Barbara (US)
- Centre National de la Recherche Scientifique (FR)
- Environmental Protection Agency (US)
- Clemson University (US)
- Wageningen University & Research (NL)
- University of California, Berkeley (US)
- University of Kentucky (US)
- Corresponding author
- Unrine, Jason M.
- Venue
- Environmental Toxicology and Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-10-21
- Volume
- 31
- Issue
- 1
- Pages
- 32-49
- ISSN
- 0730-7268 (ISSN-L)
- 1552-8618
- OpenAlex
- W2137871949
- Cited by
- 448
- References
- 96
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 10 · 2023: 16 · 2022: 18 · 2021: 31 · 2020: 31 · 2019: 35 · 2018: 31 · 2017: 27 · 2016: 54 · 2015: 54 · 2014: 61 · 2013: 40 · 2012: 22
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Licence
- Locations
- Environmental Toxicology and Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- HAL (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Physical Sciences › Environmental Science › Water Science and Technology › Environmental and Analytical Chemistry Studies
- Physical Sciences › Engineering › Biomedical Engineering › Environmental remediation with nanomaterials
- Keywords
- engineered nanomaterials, biota, sample preparation artifacts, colloidal nanoparticles, physicochemical properties, environmental fate, ecotoxicology, low-concentration analysis
- MeSH terms
- Environment, Environmental Monitoring, Environmental Pollutants, Manufactured Materials, Soil, Geologic Sediments, Nanostructures, Nanoparticles, Ecotoxicology, Biota
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