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Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line
Abstract
Summary: The use of the Cre/loxP system has greatly empowered the field of gene targeting. Here we describe the successful establishment of a novel knock‐in EGFP reporter mouse line to monitor Cre‐induced recombination in the vast majority of cell types. The value of this reporter mouse line is demonstrated by the use of a novel Tie2Cre transgenic mouse line that facilitates gene targeting in endothelial and hematopoietic cells. High efficiency of recombination was found in all endothelial cells and in the majority of hematopoietic cells but was absent in other tissues. Furthermore, in the second generation, the Tie2Cre mouse can be used to get 100% recombination of one allele, whilst allowing tissue specific in the second, therefore offering excellent efficiency. genesis 30:36–44, 2001. © 2001 Wiley‐Liss, Inc.
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Schwenk, F., Baron, U., & Rajewsky, K. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.24.5080 · Cited by 1,289
Gu, H., Zou, Y.-R., & Rajewsky, K. (1993) · Cell · doi:10.1016/0092-8674(93)90644-6 · Cited by 989
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Metadata
- Authors
- Constien, R., Forde, A., Liliensiek, B., Gröne, H., Nawroth, P., Hämmerling, G., & Arnold, B.
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2001-05
- Volume
- 30
- Issue
- 1
- Pages
- 36-44
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- OpenAlex
- W2112384663
- Cited by
- 294
- References
- 2
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- closed
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- Constien, R., Forde, A., Liliensiek, B., Gröne, H., Nawroth, P., Hämmerling, G., & Arnold, B. (2001). Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line. genesis. https://doi.org/10.1002/gene.1030
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