Regulation and differential expression of tau mRNA isoforms as oligodendrocytes mature in vivo: Implications for myelination
Abstract
Oligodendrocytes and neurons derive from the same cell type but develop distinct morphologic and functional properties as they mature in vivo. Both cells express tau protein, a developmentally regulated protein in the central nervous system. The regulation of tau has been investigated extensively in neurons but not in oligodendrocytes, so we studied regulation of tau in oligodendrocytes in vivo. The amino‐derived tau isoforms consist of isoforms with zero (A0), one (A1), or two (A2) inserts. We examined the developmental regulation of tau mRNA isoforms at the amino domain by comparing tau expression in oligodendrocytes (OLGs) isolated from 1‐ and 20‐day‐old rat brain and in age‐matched cortex, which abounds in neurons. In the rat brain, myelination peaks at 20 days. By using semiquantitative RT‐PCR, we found that OLGs and cortex from 1‐day‐old rat brain largely had amino‐derived tau isoforms with no insert, whereas OLGs from 20‐day‐old rat brain had similar levels of amino‐derived tau isoforms with no insert or with one insert. We also found that 20‐day‐old OLGs had twofold more tau mRNA levels than younger OLGs. In contrast to OLGs from 20‐day‐old rat brain, age‐matched cortex had comparable levels of A0, A1, and A2 tau amino‐derived isoforms. Further, younger and older OLGs had a reciprocal pattern of expression of both carboxy‐derived tau mRNA isoforms with either three (3R) or four (4R) repeats. In contrast, younger and older cortex expressed either 3R or 4R tau. This study showed an upregulation of tau mRNA and cell‐specific tau mRNA isoform expression in OLGs forming myelin. GLIA 37:250–257, 2002. © 2002 Wiley‐Liss, Inc.
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In oligodendrocytes, tau plays a role in process outgrowth and myelination.
“In oligodendrocytes, tau plays a role in process outgrowth and myelination [133, 136–138], however, it remains unclear at this point whether tau regulates physiological functions of astrocytes.”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [136]
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Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease
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- Authors
- Lopresti, P.
- Affiliations
- Loyola University Medical Center (US)
- Corresponding author
- Lopresti, Patrizia
- Venue
- Glia
- Publisher
- Wiley
- Published
- 2002-01-22
- Volume
- 37
- Issue
- 3
- Pages
- 250-257
- ISSN
- 0894-1491 (ISSN-L)
- 1098-1136
- OpenAlex
- W2089795127
- Cited by
- 33
- References
- 56
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 1
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Glia (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- tau protein, oligodendrocytes, oligodendrocyte maturation, myelination, developmental regulation, rat brain, cerebral cortex, cell type-specific gene expression
- MeSH terms
- Aging, Animals, Animals, Newborn, Base Sequence, Brain, Cell Differentiation, Cells, Cultured, Cerebral Cortex, Microtubules, Myelin Sheath, Nerve Fibers, Myelinated, Oligodendroglia, RNA, Messenger, Up-Regulation, tau Proteins, Rats, Sprague-Dawley, Protein Structure, Tertiary, DNA, Complementary, Gene Expression Regulation, Developmental, Protein Isoforms, Rats
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