Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes.
Open accessAbstract
We present evidence that the microtubule-associated protein tau is present in oligodendrocytes (OLGs), the central nervous system cells that make myelin. By showing that tau is distributed in a pattern similar to that of myelin basic protein, our results suggest a possible involvement of tau in some aspect of myelination. Tau protein has been identified in OLGs in situ and in vitro. In interfascicular OLGs, tau localization, revealed by monoclonal antibody Tau-5, was confined to the cell somata. However, in cultured ovine OLGs with an exuberant network of processes, tau was detected in cell somata, cellular processes, and membrane expansions at the tips of these processes. Moreover, in such cultures, tau appeared localized adjacent to or coincident with myelin basic protein in membrane expansions along and at the ends of the cellular processes. The presence of tau mRNA was documented using fluorescence in situ hybridization. The distribution of the tau mRNA was similar to that of the tau protein. Western blot analysis of cultured OLGs showed the presence of many tau isoforms. Together, these results demonstrate that tau is a genuine oligodendrocyte protein and pave the way for determining its functional role in these cells.
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- Authors
- LoPresti, P., Szuchet, S., Papasozomenos, S. C., Zinkowski, R. P., & Binder, L. I.
- Affiliations
- Molecular Geriatrics Corporation, Lake Bluff, IL 60044, USA.
- University of Chicago (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1995-10-24
- Volume
- 92
- Issue
- 22
- Pages
- 10369-10373
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC40798
- OpenAlex
- W1991483073
- Cited by
- 388
- References
- 53
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 9 · 2023: 24 · 2022: 19 · 2021: 31 · 2020: 12 · 2019: 18 · 2018: 22 · 2017: 9 · 2016: 8 · 2015: 13 · 2014: 10 · 2013: 11 · 2012: 8
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Pennsylvania
- National Institutes of Health (5p01ns024575-08, 7r01ag006969-09, 5p50ag008664-09)
- National Institute on Aging (R01-AG06969, P50-AG08664)
- National Institute of Neurological Disorders and Stroke (P01-NS-24575)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- tau protein, microtubule-associated proteins, oligodendrocytes, myelination, myelin basic protein, tau isoforms, membrane expansion
- MeSH terms
- Animals, Brain, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Myelin Basic Protein, Immunohistochemistry, Oligodendroglia, Sheep, Blotting, Western, Microscopy, Immunoelectron, tau Proteins, In Situ Hybridization, Fluorescence, Fluorescent Antibody Technique, Indirect, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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