Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes
Abstract
Recent observations indicate that several stem cells can differentiate into hepatocytes; thus, cell-based therapy is a potential alternative to liver transplantation. The goal of the present study was to examine the in vitro hepatic differentiation potential of adipose tissue-derived mesenchymal stem cells (AT-MSCs). We used AT-MSCs from different age patients and found that, after incubation with specific growth factors (hepatocyte growth factor [HGF], fibroblast growth factor [FGF1], FGF4) the CD105+ fraction of AT-MSCs exhibited high hepatic differentiation ability in an adherent monoculture condition. CD105+ AT-MSC-derived hepatocyte-like cells revealed several liver-specific markers and functions, such as albumin production, low-density lipoprotein uptake, and ammonia detoxification. More importantly, CD105+ AT-MSC-derived hepatocyte-like cells, after transplantation into mice incorporated into the parenchyma of the liver. Conclusion: Adipose tissue is a source of multipotent stem cells that can be easily isolated, selected, and induced into mature, transplantable hepatocytes. The fact that they are easy to procure ex vivo in large numbers makes them an attractive tool for clinical studies in the context of establishing an alternative therapy for liver dysfunction. (HEPATOLOGY 2007;46:219–228.)
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MSCs derived from various sources can differentiate into functional HLCs along the endodermal lineage under both in vitro and in vivo conditions in experimental animal models and humans.
“It has been reported in experimental animal models and humans that MSCs derived from various sources can differentiate into functional HLCs along the endodermal lineage under both in vitro and in vivo conditions [90–92].”
From Mechanisms, efficacy, and future perspectives of cellular-based therapies for liver fibrosis/cirrhosis: focusing on mesenchymal stromal cells (2025) · cites this paper as [90]
In vitro fully differentiated HLCs derived from CD105+ adipose tissue-hMSC, after infusion into CCl4-injured mice through the tail vein, were incorporated into the liver parenchyma and resulted in enhancements in several liver functions.
“After being infused into the CCl4-injured mouse through the tail vein, in vitro fully differentiated HLCs derived from CD105+ adipose tissue (AT)-hMSC were incorporated into the liver parenchyma, verified by human ALB immunostaining, resulting in enhancements in several liver functions [90].”
From Mechanisms, efficacy, and future perspectives of cellular-based therapies for liver fibrosis/cirrhosis: focusing on mesenchymal stromal cells (2025) · cites this paper as [90]
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“After being infused into the CCl4-injured mouse through the tail vein, in vitro fully differentiated HLCs derived from CD105 + adipose tissue (AT)-hMSC were incorporated into the liver parenchyma, verified by human ALB immunostaining, resulting in enhancements in several liver functions [90].”
- Authors
- Banas, A., Teratani, T., Yamamoto, Y., Tokuhara, M., Takeshita, F., Quinn, G., Okochi, H., & Ochiya, T.
- Affiliations
- National Cancer Center Research Institute (JP)
- Waseda University (JP)
- Corresponding author
- Ochiya, Takahiro
- Venue
- Hepatology
- Published
- 2007-07
- Volume
- 46
- Issue
- 1
- Pages
- 219-228
- ISSN
- 0270-9139 (ISSN-L)
- OpenAlex
- W1967466647
- Cited by
- 555
- References
- 0
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 13 · 2023: 12 · 2022: 20 · 2021: 23 · 2020: 27 · 2019: 21 · 2018: 28 · 2017: 28 · 2016: 36 · 2015: 30 · 2014: 42 · 2013: 58 · 2012: 52
- FWCI
- 17.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Hepatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- adipose-derived stem cells, hepatocyte differentiation, hepatocyte-like cells, CD105, hepatocyte growth factor, fibroblast growth factor, albumin synthesis, ammonia detoxification, liver transplantation alternatives, cell therapy, liver regeneration, stem cell transplantation
- MeSH terms
- Adipose Tissue, Adult, Cell Differentiation, Female, Gastrectomy, Humans, Male, Middle Aged, Stem Cells, Stomach Neoplasms, Antigens, CD, Tissue and Organ Harvesting, Hepatocytes, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells
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