Quantification of postsynaptic density proteins: Glutamate receptor subunits and scaffolding proteins
Abstract
The postsynaptic density (PSD) protein complex has long been a major target of proteomics in neuroscience. As the number of glutamate receptors on a synapse is one of the main determinants of synaptic efficacy, determining the absolute numbers of receptors in the PSD is necessary for estimating the amplitude of the excitatory postsynaptic current (EPSC) in individual synapses. Moreover, as the receptor molecules are embedded in a macromolecular complex within the PSD, stoichiometry between the receptors and other PSD proteins could help explain the functional and regional specialization of the synapses and their possible roles in synaptic plasticity. Here, I review various studies concerned with the quantification of PSD proteins.
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The number of NMDARs at spine synapses and perhaps filopodia synapses is much less variable, averaging roughly 20, and this number is largely independent of PSD size.
“The number of NMDARs at spine synapses and perhaps filopodia synapses is much less variable, averaging roughly 20, and this number is largely independent of PSD size (143, 438, 439).”
From Synaptic memory and CaMKII (2023) · cites this paper as [439]
The number of AMPARs at dendritic spine synapses is highly variable but averages roughly 50, and this number strongly correlates with the size of the PSD.
“The number of AMPARs at dendritic spine synapses is highly variable but averages roughly 50 (143, 438, 439), and this number strongly correlates with the size of the PSD.”
From Synaptic memory and CaMKII (2023) · cites this paper as [439]
Cites
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Serial Block-Face Scanning Electron Microscopy to Reconstruct Three-Dimensional Tissue Nanostructure
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Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels
Hansen, K. B., Wollmuth, L. P., Bowie, D., Furukawa, H., Menniti, F. S., Sobolevsky, A. I., Swanson, G. T., Swanger, S. A., Greger, I. H., Nakagawa, T., et al. (2021) · Pharmacological Reviews · doi:10.1124/pharmrev.120.000131 · Cited by 686 · see citing works
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“The number of NMDARs at spine synapses and perhaps fi lopodia synapses is much less variable, averaging roughly 20, and this number is largely independent of PSD size (143, 438, 439).”
BackgroundDutta, D., Jana, M., Paidi, R. K., Majumder, M., Raha, S., Dasarathy, S., & Pahan, K. (2023) · Journal of Clinical Investigation · doi:10.1172/jci161987 · Cited by 87 · see citing works
- Authors
- Shinohara, Y.
- Affiliations
- RIKEN Center for Brain Science (JP)
- Corresponding author
- Shinohara, Yoshiaki
- ORCID
- Shinohara, Yoshiaki
- Venue
- Hippocampus
- Publisher
- Wiley
- Published
- 2011-05-18
- Volume
- 22
- Issue
- 5
- Pages
- 942-953
- ISSN
- 1050-9631 (ISSN-L)
- 1098-1063
- OpenAlex
- W1984972580
- Cited by
- 33
- References
- 8
- Citations by year
- 2026: 2 · 2025: 5 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 4
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Hippocampus (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23590273)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- postsynaptic density, glutamate receptor subunits, scaffold proteins, protein stoichiometry, excitatory postsynaptic currents, synaptic plasticity, absolute protein quantification
- MeSH terms
- Animals, Carrier Proteins, Hippocampus, Membrane Proteins, Microscopy, Fluorescence, Nerve Tissue Proteins, Neuronal Plasticity, Mass Spectrometry, Receptors, N-Methyl-D-Aspartate, Receptors, Glutamate, Receptors, AMPA, Excitatory Postsynaptic Potentials, Protein Subunits, Adaptor Proteins, Signal Transducing, Mice, Rats, Post-Synaptic Density
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