Identification of DOCK4 and its splicing variant as PIP3 binding proteins
Abstract
DOCK4, a member of DOCK180 family proteins, was originally identified as a product of a gene deleted during tumor progression. Although its tumor suppression properties have been reported, the regulation mechanism of this protein has not been fully elucidated. DOCK4 shares two conserved domains called as DHR‐1 and DHR‐2 domain as other members including DOCK180. Although DHR‐1 in DOCK180 is reported to bind to PIP 3, whether that of DOCK4 exhibits similar function has yet not been examined. In a search for novel PIP 3 binding proteins by the PIP 3 analog beads binding assay, we found that DOCK4 and its novel splicing variant, whose exon1 and exon52 are different from the known one, bind to PIP 3. Binding assay using deletion mutants of DOCK4 revealed that the binding region falls into the DHR‐1 domain. These results raise the possibility that DOCK4 may be regulated by PIP 3 to exert its function. © 2008 IUBMB IUBMB Life, 60(7): 467–472, 2008
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- Authors
- Kanai, A., Ihara, S., Ohdaira, T., Shinohara‐Kanda, A., Iwamatsu, A., & Fukui, Y.
- Affiliations
- The University of Tokyo (JP)
- Kanazawa University (JP)
- Corresponding author
- Fukui, Yasuhisa
- Venue
- IUBMB Life
- Publisher
- Wiley
- Published
- 2008-05-05
- Volume
- 60
- Issue
- 7
- Pages
- 467-472
- ISSN
- 1521-6543 (ISSN-L)
- 1521-6551
- OpenAlex
- W1977663169
- Semantic Scholar
- ba4bfe4e5359e58d93d7b8277eae9d0881204dd5
- Cited by
- 12
- References
- 21
- Citations by year
- 2024: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 1 · 2014: 1 · 2013: 2
- FWCI
- 0.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- IUBMB Life (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- DOCK4, alternative splicing, tumor suppressor, phosphoinositide 3-kinase signaling, protein-protein interaction
- MeSH terms
- Carrier Proteins, Cell Line, Cloning, Molecular, Exons, Gene Expression Regulation, Humans, Models, Biological, Mutation, Protein Binding, Transfection, Alternative Splicing, Microscopy, Confocal, Protein Isoforms, GTPase-Activating Proteins, rac GTP-Binding Proteins
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