Cited by 295HIV Research and TreatmentCellular transport and secretionT-cell and Retrovirus Studies

Human immunodeficiency virus infection of CD4-bearing cells occurs by a pH-independent mechanism

Open access
McClure, M. O., Marsh, M., Weiss, R. A.·1988·The EMBO Journal

Abstract

The effect of weak bases (NH4Cl and amantadine) and carboxylic ionophores (monensin) on the infection of CD4 (T4) positive human cell lines by HIV-1 is examined. These reagents, which raise the pH of acidic intracellular organelles, fail to inhibit HIV-1 entry and the events leading to viral protein synthesis at concentrations inhibitory for low pH-dependent fusogenic enveloped viruses. The infectivity of VSV (HIV-1) pseudotypes is unaffected by weak bases at concentrations causing 95% plaque reduction of VSV in its own envelope. HIV-1 dependent cell--cell fusion (syncytium formation) occurs in medium maintained at pH 7.4-7.6, and virions are not irreversibly inactivated by incubation in acid medium. Our results show that HIV-1 entry and membrane fusion do not require exposure to low pH. The production of infectious HIV-1 particles, however, is inhibited in cells treated with NH4Cl.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1002/j.1460-2075.1988.tb02839.x

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

3 of the 46 references couldn’t be matched to a known paper.

Research works printed but not linked: 7

  1. Galloway,C.J., Dean,G.E., Marsh,M., Rudnick,G. and Mellman,I. (1983) Proc. Natl. Acad. Sci. USA, 80, 3334-3338.
  2. Haywood,A.M. (1988) In Wilschut,J. and Hoekstra,D. (eds), Cellular Membrane Fusion. Marcel Dekker, New York (in press).
  3. Marsh,M., Wellsteed,J., Kern,H., Harms,E. and Helenius,A. (1982) Proc. Natl. Acad. Sci. USA, 79, 5297-5301.
  4. Montagnier,L. et al. (1984) In Gallo,R.C., Essex,M. and Gross,L. (eds), Human T-Cell Leukemia -Lymphoma Viruses. Cold Spring Harbor Lab- oratory Press, Cold Spring Harbor, NY, pp.
  5. Ohkuma,S. and Poole,B. (1978) Proc. Natl. Acad. Sci. USA, 75, 3327- 3331.
  6. Poste,G. and Pasternak,C.A. (1978) In Poste,G. and Nicholson,G.C. (eds), Membrane Fusion. Elsevier/North Holland, Amsterdam, pp. 305 -367.
  7. Skehel,J., Bayley,P., Brown,E., Martin,S., Waterfield,M., White,J., Wilson,I. and Wiley,D. (1982) Proc. Natl. Acad. Sci. USA, 79, 968-972.

Cited by

Sorted by citations

Authors
McClure, M. O., Marsh, M., & Weiss, R. A.
Affiliations
Institute of Cancer Research (GB)
Venue
The EMBO Journal
Published
1988-02-01
Volume
7
Issue
2
Pages
513-518
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W108460990
Cited by
295
References
46
Citations by year
2026: 1 · 2025: 5 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 7 · 2012: 11
FWCI
6.89
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Funders
  • Wellcome Trust
  • Medical Research Council
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
Keywords
HIV-1 infection, CD4+ T cells, membrane fusion, syncytium formation, ammonium chloride, amantadine, monensin, carboxylic ionophores, weak bases, viral protein synthesis
MeSH terms
Antigens, Differentiation, T-Lymphocyte, Cell Line, Cell Transformation, Viral, HIV, Humans, Hydrogen-Ion Concentration, Viral Envelope Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review