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Enhancer‐like interferon responsive sequences of the human and murine (2′‐5′) oligoadenylate synthetase gene promoters.
Open accessAbstract
The human (2'-5') oligo(A) synthetase gene contains two independent cis-acting DNA elements, A and B, which act as transcriptional enhancers. Element A alone is not activated by IFN treatment. Element B alone confers IFN-inducibility to the herpes tk promoter. Two murine (2'-5') oligo(A) synthetase genes were isolated and their promoter sequences show high conservation of element A and B. A synthetic oligonucleotide, containing 16 bp of the human element B, or 14 bp of the homologue murine element B, was linked to a TK-CAT construct. These oligonucleotides were shown to be sufficient to activate the TK promoter in the presence of IFN. When multiple repeats of the interferon-responsive sequence (E-IRS) were cloned in 5' of the TK promoter, the activation ratio was increased. In vitro, specific binding of nuclear protein(s) is observed to the radiolabelled synthetic human E-IRS. This binding is competed by the addition of cold synthetic mouse E-IRS or fragments of genomic DNA containing the E-IRS.
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Metadata
- Authors
- Cohen, B., Peretz, D., Vaiman, D., Benech, P., & Chebath, J.
- Affiliations
- Weizmann Institute of Science (IL)
- Venue
- The EMBO Journal
- Published
- 1988-05-01
- Volume
- 7
- Issue
- 5
- Pages
- 1411-1419
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC458391
- OpenAlex
- W2344893292
- Cited by
- 180
- References
- 35
- Citations by year
- 2024: 1 · 2020: 1 · 2018: 1 · 2015: 1 · 2014: 1 · 2013: 1
- FWCI
- 11.64
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- 2',5'-oligoadenylate synthetase, enhancers, cis-acting DNA elements, interferon induction, promoter elements, nuclear protein binding, sequence conservation
- MeSH terms
- Animals, Base Sequence, DNA, Enhancer Elements, Genetic, Multigene Family, Histocompatibility Antigens, Humans, Interferons, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Sequence Homology, Nucleic Acid, 2',5'-Oligoadenylate Synthetase, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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